Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 34 for “"PLK1"”.
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Inhibition of PLK1 by p53 and by PLK1-targeted drugs
Polo like kinase 1 (PLK1) has many functions in the orchestration of cell division. It is an oncogene and its overexpression is associated with poor prognosis. PLK1 is a cell cycle-dependent protein; its levels are low in the early phases of the cell cycle and increase as cells go through the …
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Development of Non-Atp Competitive, Plk1-Selective Inhibitors
<p>Polo like kinase 1 (PLK1) is a member of a conserved family of serine/threonine kinases that plays an important role in the regulation of mitotic progression. PLK1 is over-expressed in a number of tumor types, thus making it a good therapeutic target. Because other PLK family members are …
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The role of PLK1 in NLRP3 inflammasome activation
… form of cell death. The kinase PLK1 plays an important and well-characterised role in mitosis, but the function of this protein in interphase has been poorly studied so far. I showed that mitosis and NLRP3 inflammasome activation are mutually exclusive events in murine BMDMs …
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The PLK1 and NLRP3 interactome in NLRP3 inflammasome activation
… work has identified the Polo-like kinase (PLK1) as a potential regulator of the NLRP3 inflammasome pathway. Using two different methods, I showed that PLK1 interacted with NLRP3 and that that interaction was enhanced during NLRP3 inflammasome activation. Moreover, I identified the potential …
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The role of PLK1 in Hepatitis B Virus (HBV) infection of the liver
… I also identify Polo-like-kinase 1 (PLK1) as a proviral factor in HBV pathogenesis. I demonstrate by loss of function as well as gain of function approaches that PLK1 inhibition suppresses viral replication both in vitro and in vivo. I also show that HBc is a phosphorylation substrate …
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Investigation of the role of p53 in protecting cancer cells against PLK1 inhibitors
The protein kinase Polo-like kinase-1 (PLK1) is an essential driver of mitosis and is oncogenic when expressed at elevated levels. Therefore, a number of inhibitors of PLK1 have been developed as potential anti-cancer therapeutic agents. While these inhibitors undergo clinical evaluation there has …
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G2/M checkpoint associated repression of polo-like kinase-1 mediated by the tumour suppressor, p53
Polo-like Kinase-1 (PLK1) is an important mediator of the G2/M phase of the cell cycle that is down-regulated in a DNA damage dependent manner. In cancer cells PLK1 is overexpressed and allows for continued proliferation of the cell by overriding this checkpoint. Here I show that PLK1 is …
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Polo-Like Kinase 1 and Smooth Muscle Cells in Coronary Artery Bypass Conduits and Coronary Arteries Following Angioplasty
… the role of polo-like kinase (PLK)-1 in the PLK1/pPLK1-pCDK1-p-Histone H3 pathway in mediating mitotic progression of smooth muscle cells (SMCs) from human IMA and SV in the development of IH leading to veingraft disease. We also show in vivo data in support of increased immunopositivity …
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Epithelial to Mesenchymal Transition As A Predictor of Response to Polo-Like Kinase 1 Inhibition-Induced Apoptosis In Non-Small Cell Lung Carcinoma
… or metastatic NSCLC. Polo-like kinase 1 (PLK1), involved in the regulation of mitotic processes and the response to DNA damage, is overexpressed in NSCLC. Inhibiting PLK1 may be an effective treatment for NSCLC patients as it is involved in the mechanisms of resistance to several …
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Determining Mechanisms of Response to Polo-Like Kinase 1 Inhibition In Non-Small Cell Lung Cancer
… of drugs. We identified polo-like kinase 1 (Plk1) as the most promising target for further investigation based on a subset of sensitive cell lines and inhibitors that were in advanced clinical development. To identify potential biomarkers of response and mechanisms of Plk1 inhibitor-induced …
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EXPLORING THE CROSSTALK BETWEEN POLO-LIKE KINASE 1 AND Eph-FAMILY OF RECEPTORS IN CELL DIVISION IN PANCREATIC CANCER MODEL
… leads to upregulation of Polo-like kinase 1 (PLK1) a mitotic kinase and Receptor tyrosine kinases (RTKs) which promotes metastatic behaviour. Of the majority of Eph receptors EphA2 is overexpressed in many cancers and in PDAC as well. A wealth of literature has reported an increased expression …
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Maintenance of Genomic Integrity by RSF1 Chromatin Remodeler
… mitotic kinetochores and directly interacts with PLK1 9 B. RSF1 plays a crucial role in PLK1 deposition and activation at the mitotic kinetochores 16 C. Phosphorylation of RSF1 at Ser1375 by CDK1 is necessary and sufficient for PLK1 docking to kinetochores 22 D. PLK1-dependent secondary …
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Lokalisation, Regulation und Interaktionen muriner DNA-Replikationsproteine
… mitotischen Ereignissen, der POLO-like kinase1 (Plk1), phosphoryliert werden, wurden in vitro-Kinase-Assays mit Wildtyp-Plk1 bzw. der Kinase-defizienten Mutante Plk1 (K82M) als Negativ-Kontrolle und potentiellen Targetproteinen durchgeführt. Orc2, Cdc7 und Cdc45 konnten als in vitro-Substrate für …
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The role of cell division during tissue morphogenesis
… points towards the center of the embryo in a PLK1- and PLK4-dependent manner. Lastly, a local drug delivery system was developed in zebrafish embryos to target a PLK1 inhibitor to the centrosome. Through this system, it was revealed that centrosomal PLK1 is responsible for spindle organization …
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THERAPEUTIC POTENTIAL OF TARGETING THE FLNA-REGULATED WEE1 KINASE PATHWAY IN ADRENOCORTICAL CARCINOMAS.
… for those lacking FLNA. Polo-like kinase 1 (PLK1) and cyclin-dependent kinases (CDKs) 1/2/4 are among the most overexpressed cell cycle-related genes in ACC human samples. Previously, the efficacy of the polo-box domain (PBD)-targeting PLK1 inhibitor Poloxin has been demonstrated in ACC cell …
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Characterizing the RanGAP1-RanBP2 complex in mitosis
… RanGAP1 Komplexen (Topo II alpha, TACC2, CKAP-5, Plk1, USP7, PIAS1). Die meisten davon konnten entweder in vitro mit rekombinanten Faktoren (TACC2, Plk1) oder als endogene Proteine, die mit mitotischem RanGAP1-RanBP2 Komplex als Quelle der Sumo E3 Ligase-Aktivität assoziiert waren (TopoII alpha, …
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Functional characterization of the novel centrosomal protein Nlp (ninein-like protein)
… centrosomal substrate of Polo-like kinase 1 (Plk1), an important regulator of mitosis whose activity is required for centrosome maturation. Nlp interacts with two distinct gammaTuRC components, gamma-tubulin and hGCP4, and stimulates MT nucleation. Plk1 phosphorylates Nlp and disrupts its …
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The Multifunctional Kinase Bub1 Acts as a Signaling Hub for the Spindle Checkpoint
… Here I show that Bub1 binds another kinase Plk1, forming a kinase complex. Phosphorylation of Cdc20 by Bub1-Plk1 not only inhibits APC/CCdc20 in vitro, but also is required for proper spindle checkpoint function in HeLa cells.
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Combined Inhibition of Ddr1 and Cdk4/6 Induces Synergistic Effects In Er-Positive, Her2-Negative Breast Cancer With Pik3Ca/Akt1 Mutations
… further induced cell cycle arrest, decreasing PLK1, cyclin E2, and RB phosphorylation in <em>PIK3CA </em>and<em> AKT1</em> mutant cell lines. <em>In vivo</em>, 7rh significantly enhanced the antitumor efficacy of palbociclib. Similarly, reverse phase protein array (RPPA) analysis of the …
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