Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 11 of 11 for “"PLASMID STABILITY"”.
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Plasmid stability of pUB110 and pUB110-derived plasmids in Bacillus sphaericus 2362
… To evaluate the use of the Staphylococcus aureus plasmid pUB110 (4.5 kb) for this purpose, the stability (ability of the plasmid to remain intact and divide into daughter cells during bacterial cell division) of this plasmid and four plasmids derived from pUB110 was examined. The recombinant …
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Mathematical and experimental approaches to the dimer catastrophe theory
Multicopy plasmids rely on random distribution for stable inheritance by daughter cells at division. Threats to plasmid copy number increase the probability of plasmid loss, which can be detrimental to both plasmid and host. Plasmid dimers emerge through homologous recombination. Dimers have two …
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Role of airborne soil particulate in transfer of Salmonella spp. to tomato blossoms and consequential fruit contamination
… were compared for bioluminescence production and plasmid stability. S. Newport had the best bioluminescence properties but no difference was observed between strains for plasmid stability. Imaging of soil particulate - S. Newport mixture inoculated blossoms, indicated that the event led to …
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Improvement of engineered thermostable xylanase production via immobilization of recombinant escherichia coli onto graphene oxide
… ~-galactosidase activity) and 72.6% increase in plasmid stability compared to free cells. The xylanase concentration was 0.482 Vlml, representing 99.4% of the predicted value (0.485 U/ml) and l.71-fold higher than the value before optimization process (0.283 U/ml). The occurrence of cell lysis …
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Targeted destruction of intracellular DNA using a CRISPR-based genetic device that can be carried indefinitely in the host genome
… DNAi activity, and a reprogrammable targeting plasmid, which encodes the CRISPR array specifying the DNA target(s). The device is stable in the OFF state, with >98% of cells retaining a low-copy DNA target over the course of an 8-hr experiment. Upon DNAi activation, the target plasmid is lost …
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Toxin-antitoxin systems in bacteria : targets, mechanisms, and specificity
… in a number of important processes, including plasmid stability, phage resistance, persistence, and virulence. Yet, there are a number of unanswered questions about these genetic modules. What are the cellular targets of toxins? How do antitoxins antagonize their cognate toxins? Do toxins and …
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Engineering Zymomonas mobilis for the production of biofuels and other value-added products
… that mutations in the AddB protein increase plasmid stability and can reduce cellular aggregation in these strains. Consistent with previous research, we found that reduced xylitol production improves xylose fermentations in Z. mobilis. We also found that increased transketolase activity and …
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Conjugative transfer and expression of an integrative and conjugative element in Pectobacterium atrosepticum : A thesis submitted in partial fulfilment of the requirements for the Degree of Doctor of Philosophy at Lincoln University
… of HAI2 core genes and genes related to plasmid stability. Absence of HAI2 excision induced downregulation of transcription pathways energy use. Changes in HAI2 excision impacted the expression of HAI5, HAI6 and HAI10, MGEs also integrated in Pba SCRI1043 chromosome. In summary, we …
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Matrix Gene Splicing and Emergence of Highly Pathogenic Avian Influenza
… One of the more common systems utilizes eight plasmids where the cDNA of all eight viral genomic segments is cloned into the bidirectional expression vector pHW2000. This vector encodes the human RNA polymerase I promoter in the reverse direction and the human cytomegalovirus RNA polymerase II …
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Microbial monitoring and degradation of lindane in soil
… also indicate the potential role of increased plasmid stability. In a third and final experimental part of this work, an alternative lindane removal technique was proposed, making use of the catalytic reduction of HCH over a metal catalyst, namely palladium (Pd[0]). Since specific surface area …
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ΜΕΛΕΤΗ ΤΩΝ ΦΥΣΙΚΩΝ ΠΛΑΣΜΙΔΙΩΝ ΤΟΥ ΣΤΕΛΕΧΟΥΣ ATCC 10988 ΤΟΥ ΑΙΘΑΝΟΛΟΠΑΡΑΓΩΓΟΥ ΒΑΚΤΗΡΙΟΥ ZYMOMONAS MOBILIS
FIVE NATIVE PLASMIDS OF Z. MOBILIS STRAIN ATCC 10988 HAVE BEEN ISOLATED, NAMELYPZMO2, PZMO3, PZMO5, PZMO6 WITH SIZE 1,70, 1,85, 2,70, 16,7, AND 31,8 KB RESPECTIVELY. RESTRICTION MAPPING (PARTIAL) OF ALL THESE PLASMIDS WAS FOLLOWED AND THEIR HOMOLOGY LEVELS WERE DETERMINED. SIMILARLY, THEIR HOMOLOGY …