Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 28 for “"PKR"”.
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Roles of PKR in High Grade Serous Ovarian Cancer
… than primary tumor growth. Protein kinase R (PKR; EIF2AK2) is classically described as a stress-activated kinase within the integrated stress response (ISR); however, its role in ovarian cancer metastasis remains poorly defined. In this study, we investigated the functional contribution of PKR …
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Regulation of Pkr Activation by Pact and Trbp In Response to Stress Signals
<p> PKR (protein kinase, RNA activated) is an interferon (IFN)-induced serine-threonine protein kinase, which plays a crucial role in IFN's antiviral and antiproliferative actions. In addition, PKR plays a regulatory role in signal transduction leading to apoptosis in response to a variety of …
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THE ADENOVIRUS E1B-55K AND E4ORF6 PROTEINS LIMIT PKR-MEDIATED EIF2A PHOSPHORYLATION DURING THE LATE PHASE OF INFECTION
… eIF2α by the dsRNA-dependent protein kinase R (PKR) during the late phase of infection. The phosphorylation of eIF2α systematically fluctuated throughout the viral life cycle. These fluctuations most likely reflect cellular responses to discrete viral events. Viral DNA replication was identified …
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West Nile virus versus the host cell: Identification of host factors that modulate infection
… infections. One such molecule, the dsRNA sensor PKR, has been shown to be important for IFN-beta induction. Therefore, to determine whether PKR was involved in the recognition of WNV infection, cells lacking PKR were infected with WNV and assayed for IFN production. Interestingly, PKR-null cells …
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Characterization of the anti-viral effects inhibited by the vaccinia virus K1 protein
… double-stranded RNA activated protein kinase (PKR) pathway, is important in sensing the presence of intracellular viral genomes and their products. PKR is activated by dsRNA, a by-product of many virus infections, and exhibits its anti-viral effects in part via inhibition of protein synthesis …
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Organocobalt Mediated Cyclizations and Biochemically Inspired Isomerizations - Method Development and Mechanistic Studies.
… early to mid-2010s of the Pauson-Khand Reaction(PKR). Chapter two focuses on our work utilizing x-ray absorption spectroscopy(XAS) to characterize reaction in- termediates of the intermolecular PKR. Chapter three highlights our group's work developing the use of N 2 O as a new promoter of the PKR …
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The response of eIF2α kinases to plant virus infection
… kinase (GCN2) and protein kinase RNA-activated (PKR). The aim of this research was to further elucidate the role of eIF2α phosphorylation in plants, with a particular focus on the response of eIF2α kinases, including a putative PKR, to virus infection. It is not clear whether in plants, as in …
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Investigation into the mechanisms responsible for muscle atrophy in cancer cachexia
… form of the dsNRA dependent protein kinase (PKR), as well as the phosphorylated form of the translation initiator elF2a, possibly through the release of calcium, at the same concentration as that inhibiting protein synthesis. Inhibition of PKR reversed the inhibition of protein synthesis by …
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Studies on the Mechanism of Polyamine-and Prothrombin kringle-2-induced Degeneration of Neuron and Microglia
… mediators. Intracortical injection of pKr-2 caused significant loss of cortical neurons in vivo after seven days, as evident from Nissl staining and immunohistochemical analysis using the neuronal-specific nuclear protein (NeuN) antibody. In parallel, pKr-2-activated microglia and ROS …
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Ρύθμιση της έκφρασης του IRES (εσωτερική θέση πρόσβασης του ριβοσώματος) του ιού της ηπατίτιδας C
… υπάρχουν ισχυρές ενδείξεις ότι η ενεργοποιημένη PKR ρυθμίζει θετικά την ενεργότητα του IRES ενώ η καταστολή της έκφρασης της ενδογενούς PKR έχει το αντίθετο αποτέλεσμα. Επιπλέον, καταλήξαμε στο συμπέρασμα ότι η NS5A μεσολαβεί ανασταλτικά στην IRES-εξαρτώμενη μετάφραση και συνδέεται με την …
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Characterization of the vaccinia virus ankyrin repeat protein K1 on NF-κB inhibition and its contributions to viral pathogenesis
… double-stranded RNA-dependent protein kinase (PKR) activation. A consequence of this function is that K1 inhibits PKR-induced NF-κB activation during VACV infection. However, transient expression of K1 also inhibits Toll-like receptor (TLR)-induced NF-κB activation. This suggested that K1 had a …
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Characterization of Innate Immune Mechanisms Against RVFV and a Potential Target for Antivirals
… in the S- segment promotes degradation of PKR and general transcription shutoff including IFN-β mRNA synthesis. Currently, no effective measures have been developed to prevent or treat RVFV disease and very little is understood about what takes place during the initial stages of RVFV …
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Using high-throughput proteomics to identify novel engineering targets for enhanced rAAV production in HEK293 cells
… by selecting three targets, namely C11orf96, PKR and TFEB, for the rational design of an improved rAAV-producing HEK293-VP 2.0 cell line. Preliminary results showed that knockdown of C11orf96 or PKR could enhance rAAV6.2 yields by up to 100% and 50%, respectively.
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Inosine-containing mRNA induces an innate immune response and is translated with lower efficiency
… attributed to the sensors RIG-I, MDA5, TLR3, or PKR.
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Mikrobielle Ökologie N-Acyl-L-Homoserinlacton-produzierender Bakterien in der Rhizosphäre von Tomatenpflanzen
… IsoF using an AHL-sensor strain P. putida F117 pKR-C12. AHL was produced in effective concentrations in the rhizosphere of tomato plants and influenced the bacterial rhizosphere population. However, the AHL-production had no influence on the colonization behaviour of the AHL-producing strains S. …
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Mechanisms of toxicity in cell models of FSHD
… with induction of the pro-apoptotic PKR dsRNA response pathway. By high- throughput sequencing RNAs immunoprecipitated with anti-dsRNA antibodies I determined that DUX4-induced dsRNAs appeared to originate from intergenic loci that are enriched for Alu and LINE-1 elements, endogenous …
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Comparative Analysis of Antiviral Responses in Mouse Embryonic Stem Cells and in Mesenchymal Stem Cells
… Furthermore, IFNP induced expression of ISG 15, PKR and dsRNA receptor genes that play key roles in antiviral responses. In conclusion, mESCs are deficient in type I IFN expression, but they have functional mechanisms that mediate the antiviral effects of type I IFN.</p>
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The Potential Role of Insulin (and Stress Response) Pathway Components In Breast Cancer Development and Progression
… in breast cancer cells. However, an increase in PKR protein levels and endogenous kinase activity in breast cancer cells led us to examine PACT protein levels. PACT protein levels are also increased in several breast cancer cell lines. In contrast, PACT mRNA levels are increased in the …
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Mechanisms Regulating Amyloid Processing Enzymes in HIV-Associated Neurotoxicity
… via proteasomal degradation. We also explored PKR-like Endoplasmic Reticulum Kinase (PERK), one of three effectors of the unfolded protein response (UPR), as a potential regulator of APP secretases as it has an established role in modulating BACE1. Using PERK inhibitors, we found that the …
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