Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 30 for “"PIP3"”.
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Upregulation of PIP3-dependent RAC Exchanger 1 (P-Rex1) Promotes Prostate Cancer Metastasis
Activation of Rac by guanine-nucleotide exchange factors (GEFs) at the leading edge of the cell plays an important role in directional cell migration, a critical step of tumor metastasis cascades. We investigated the role of P-Rex1, a novel specific GEF for Rac, in human prostate cancer metastasis. …
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DISSECTING THE FUNCTION AND INTERACTOME OF PLEKHS1 IN PROSTATE TUMORIGENESIS
… (PI(3,4,5)P3 or PIP3) is the lipid product and key signalling output of Class I phosphoinositide 3-kinases (PI3Ks) and acts as a coordinator of a huge network that controls cellular processes such as metabolism, cell growth and movement. Mutations that augment the …
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Nanoscale Organization of The Small Gtpase Rac1
… (PA) and phosphoinositol (3,4,5)-trisphosphate (PIP3), resulting in nanoclusters enriched in these anionic lipids. I found these lipids to be structurally important as depleting the PM of PA or PIP3 impaired both Rac1 PM targeting and nanoclustering. Lipid binding specificity of Rac1 is encoded …
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Regulation of LFA-1 on T cells by phosphoinositide signalling
… the downstream phosphoinositide PtdIns(3,4,5)P3 (PIP3). To investigate how PI3K regulates LFA-1, I optimised CRISPR/Cas9-mediated mutagenesis in T cells, and designed a retroviral library of CRISPR/single guide RNAs targeting all known and potential PIP3-binding proteins. Using this library, I …
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The Effect of Manipulation of the Phosphoinositide 3-kinase Pathway on Axon Regeneration in vivo
… to phosphatidylinositol (3,4,5)-tris-phosphate (PIP3). PTEN acts as a pathway regulator, converting PIP3 back into PIP2. PIP3 is a second messenger molecule for several pathways, including the mammalian target of rapamycin (mTOR) pathway, which has been demonstrated to promote axon regeneration …
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Dock180/Elmo proteins are critical regulators of phagocytosis,macropinocytosis and chemotaxis in Dictyostelium
… in phosphatidyl inositol (3,4,5) tris phosphate (PIP3) concentration in the plasma membrane at the cell's leading edge. PIP3 binding domain containing RacGEF's are thought to mediate the cAMP signal through the activation of Racs to localised actin polymerization, which results in further …
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The Role of the Mammalian Target of Rapamycin (mTOR) in Cytotoxic T Lymphocytes
… the phosphatidylinositol (3,4,5)-trisphosphate (PIP3) signalling effector PKB in CTL. A striking observation was that mTOR inhibitors allow T cells to accumulate very high levels of PIP3 and cause T cells to hyperactivate the serine/threonine kinase PKB and moreover reprogram PKB activation. PKB …
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Gene therapy for axon regeneration
… the hypothesis that low axonal PtdIns-3,4,5-P3 (PIP3) signalling, due to the absence of growth-promoting receptors in the axon, contributes to regenerative failure in the CNS. This chapter investigates whether overexpression of phosphoinositide 3-kinases (PI3Ks), which generates PIP3, can promote …
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Src Homology 2 Domain-Containing 5’-Inositol Phosphatase-2 (Ship2) Is An Effector of Lymphatic Dysfunction
… is a phosphatidyIinositol (3,4,5) triphosphate (PIP3) 5’-phosphatase that negatively controls PIP3 levels thereby inhibiting the PI3K/AKT signaling, a pathway implicated in various lymphatic disorders. Our studies confirm this inhibitory role of SHIP2 against PI3K/AKT in lymphatic endothelial …
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NOVEL REGULATORS OF GPVI-MEDIATED PLATELET ACTIVATION
… of phosphatidylinositol (3,4,5)-trisphosphate (PIP3) subsequently leading to the recruitment of pleckstrin homology (PH) domain-containing proteins to the plasma membrane. We performed a proteomic screen to identify proteins that interacted with PIP3 using PIP3 beads, and among these proteins, …
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Redefining the specificity of phosphoinositide-binding by human PH domain-containing proteins
… reported. For 8 proteins, PI(3,4,5)P3 (PIP3) binding affinity was further validated in vivo by insulin stimulated PIP3 production on the plasma membrane and subsequent translocation. In Chapter 3, I describe a probabilistic sequence comparison algorithm, called recursive functional …
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Studies of the PTEN tumour suppressor in endometrial cancer.
… phosphatidylinositol 3,4,5-trisphosphate (PIP3), which activates protein kinase B/Akt. Constitutive activation of Akt has been found in cells that lack functional PTEN, thus by dephosphorylating PIP3, this enzyme modulates several cellular functions e.g. proliferation, differentiation and …
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Redox Regulation of Akt Phosphorylation in PTEN-/- Mouse Embryonic Fibroblasts
… not dependent on any alterations in the level of PIP3, an important secondary messenger regulating Akt phosphorylation. Instead, the Akt molecules present in the cytosol are the primary target of this O2.- -mediated regulation, which is achieved via PP2A-dependent dephosphorylation. Furthermore, …
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Dissection of CD2AP functions in epithelial cells
… localizing PI3K to cell-cell boundaries and for PIP3 accumulation along lateral membranes. CD2AP is also necessary for actin assembly in epithelial cells, and I demonstrated that perturbing actin assembly with drugs also leads to an expansion of apical and basal domains that comes at the expense …
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Evaluating Phosphatidylinositide as Lipid Biomarkers for Hyperglycemia-related Triple-negative Breast Cancer Aggressiveness
… TNBC, this work identifies PI4P, PI(4,5)P2 and PIP3 lipids as potential biomarkers and points toward new strategies for risk stratification and therapeutic intervention.
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Ca²⁺ and phosphoinositides regulations in α-actinin -4 F-actin binding.
… of α-actinin-4 by phosphoinositides (PIP2 and PIP3), the calmodulin-like domain and Ca²⁺ , determination of the three-dimensional structure of the CH2 domain in solution and identification of the phosphoinositide binding site on the CH2 domain. In order to investigate the F-actin binding …
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alpha-Actinin-4 and the podocyte: Implications for focal segmental glomerulosclerosis
… with actin, while binding of PIP2 and PIP3 enhances this interaction. High-affinity variants of alpha-actinin-4 are very static compared to the wildtype protein, and are insensitive to regulation by calcium or phosphoinositides. Such cytoskeletal dysfunction severely impairs the …
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A novel 4-valued discrete network for the analysis of signalling events integrating protein interaction and gene expression data applied on hematopoietic diefferentiation
… of EpoR induced hematopoesis, like AKT, PIP3, Pi3K, Lyn, ERK.
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dLipin-A Link between Lipid Metabolism, Glucose Homeostasis and Growth in Drosophila melanogaster
… with phosphatidylinositol (3,4,5)-trisphosphate (PIP3) synthesis. Mosaic analysis revealed that cell-autonomous loss of dLipin activity in fat the body negatively affects cell growth. Genetic interaction experiments indicated that dLipin and the insulin pathway regulate adipogenesis in an …
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Invesitagating the role of the guanine nucleotide exchange factor P-Rex1 in cell invasion
… by growth factor mediated accumulation of PIP3. P-Rex1 expression is shown to be upregulated in a number of melanoma derived cell lines compared to normal human melanocytes. Ectopic overexpression of P-Rex1 enhances the membrane ruffling and invasion of melanoma derived cell lines, while in …
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