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Showing 1 to 20 of 52 for “"PIP2"”.
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PIP2 Modulation of Afterhyperpolarizations in Magnocellular Supraoptic Neurons
… of AHPs, specifically how the phospholipid, PIP2 can activate and modulate the mAHP and sAHP. The major discovery is that the mechanisms that generate mAHP and sAHP are different between OT and VP neurons. PIP2 depletion <em>via </em>wortmannin in the cells abolishes the mAHP and sAHP of OT …
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Divalent Cation- and Cholesterol-Induced Perturbation in Pip2 Lateral Organization in Model Membranes - Cluster formation, Phase Partitioning, and PiP2-Protein interactions
… phosphatidylinositol 4,5-bisphosphate (PIP2), has been documented in numerous reports. As large number of proteins bind PIP2 but much more is known about the biochemical interactions between PIP2 and purified proteins in vitro than about the mechanisms of dynamic local regulation of …
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MYO1C Binds to PiP2 With High Affinity Through a Putative Ph Domain
… tail domain binds tightly and specifically to PIP2 in a non-cooperative manner. It binds with slightly higher affinity to Ins(1,4,5)P3 as well as other inositol phosphates which may act as inhibitors to membrane binding in the cell. By sequence and secondary structure analysis, we identified …
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PIP5-Kinase Dependent PIP2 Generation Impairs T Cell Activation by Rigidifying the Actin Cortex
… by phosphatidylinositol 4,5-bisphosphate (PIP2) (Sechi et al, 2000; Yin et al., 2003; Logan et al., 2006). How actin dynamics is regulated by PIP2 in T cell activation is unclear. Generation of PIP2 at the plasma membrane can be accomplished by the three isoforms of the type I …
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A study on PIP5Kalpha degradation by Nedd4-1-mediated ubiquitination
Phosphatidylinositol 4,5-bisphosphate (PIP2) is a critical lipid mediator of diverse membrane processes including vesicle trafficking, actin cytoskeleton dynamics and cell signaling at the cell surface, indicating significance of spatiotemporal regulation and underlying mechanism of PIP2 …
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Beteiligung der Phospholipase D an der Regulation der Synthese von Phosphatidylinositol-4,5-bisphosphat durch Arf- und Rho-GTPasen
… of phosphatidylinositol 4,5-bisphosphate (PIP2) by PIP-5-kinase. Overexpression of PLD isoforms in HEK-293 cells led to an increase in PIP-5-kinase activity and to elevated PIP2 levels in intact cells. As both PLD and PIP-5-kinase are stimulated by the GTPases Arf1 and RhoA, we investigated …
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Crosstalk Between Calcium Signaling and Lipid Metabolism at Endoplasmic Reticulum-Plasma Membrane Junctions
… of phosphatidylinositol 4,5-bisphosphate (PIP2) at the plasma membrane (PM). To enable subsequent signaling activation and maintain cellular homeostasis, it is necessary to replenish the consumed PIP2. However, the molecular mechanisms underlying PM PIP2 replenishment after hydrolysis …
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Differential Regulation of Phosphatidylinositol 4-Phosphate 5 Kinase Beta during Hypertonic and Oxidative Stress
… The phosphatidylinositol derivative, PI(4,5)P2 (PIP2)is an essential mediator of cellular processes such as signal transduction, membrane traffic, actin cytoskeleton, ion homeostasis, growth and apoptosis. Despite the importance of this signaling molecule in controlling a diverse array of …
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Functions of Phosphatidylinositol 4-Phosphate 5 Kinases in Actin Cytoskeletal Regulation During Phagocytosis
Phosphatidylinositol (4,5)-bisphosphate (PIP2) is a crucial signaling phosphoinositide at the plasma membrane (PM) which mediates a variety of biochemical activities and cellular functions. It is primarily synthesized by type I phosphatidylinositol 4-phosphate 5-kinases (PIP5Ks) through the …
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Membrane binding properties of Disabled-2
… to phosphatidylinositol 4,5-bisphosphate (PIP2) at the inner leaflet of the plasma membrane and helps co-localization with clathrin coats. Dab2, released from platelet alpha granules, inhibits platelet aggregation by binding to the °IIb? integrin receptor on the platelet surface through an …
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Einfluss der intrazellulären Cl- Konzentration auf den epithelialen Na+Kanal
… Bindung von ENaC an das anionische Phospholipid PIP2 (Phosphatidylinositol-4,5-bisphosphat) schien für die Aktivität von ENaC essentiell zu sein. Eine Veränderung der PIP2-Bindestellen im beta- und gamma-NH2-Terminus führte zu einer Verminderung der Amilorid-hemmbaren Na+-Leitfähigkeit und der …
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The membrane lipid composition regulates talin mediated integrin activation
… change. This emphasizes the importance of PIP2 in converting talin to a conformation optimized for interactions with integrin cytoplasmic tails. Moreover, a phenylalanine-rich region in F2 serves as a hidden hydrophobic anchor was initially indicated by computational simulation and later …
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Phosphatidylinositol 4-phosphate 5-kinase Involved in Neuroglial Toll-like Receptor Signaling
… that phosphatidylinositol 4,5-bisphosphate (PIP2), a membrane lipid produced mainly by the type I phosphatidylinositol 4-phosphate 5-kinase (PIP5K) family members, was necessary for TLR4 signaling. However, details of the PIP5K-mediated PIP2 production pathway and its direct regulatory effect …
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Structural Studies of the Cold and Menthol Sensing Ion Channel TRPM8
… phosphatidylinositol 4,5- bisphosphate (PIP2) as a cofactor, which allosterically couples with cooling agonists for channel activation. Since the cloning of trpm8 gene in 2002, substantial amount of functional studies has dedicated to understanding the molecular basis of ligand …
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Ca²⁺ and phosphoinositides regulations in α-actinin -4 F-actin binding.
… first α-helix of the CH2 domain. In addition, a PIP2 binding site has been mapped on a region adjacent to actin-binding site-3. These observations imply the F-actin binding activity would be regulated by phosphoinositides. Five mutations of α-actinin-4, K122N, an alternative splice variant, …
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Escherichia Coli as a New Platform for the Study of Phosphoinositides
… and phosphatidyl inositol (4,5) diphosphate (PIP2), and is easily controlled trough the addition of inositol to the growth media of the bacterium. As an example application of my system, I use it to confirm the role of PIP2 binding in the non-conventional protein export of human basic …
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How to Mend a Broken Heart: Massive Endocytosis and the Role of Lipidic Forces in Membrane Trafficking
… and phosphatidylinositol 4,5-bisphosphate (PIP2) signaling. Both forms of MEND do not rely on classical endocytic proteins such as clathrin or dynamin and appear independent of the cytoskeletal or extracellular matrix. A hypothesis emerged implicating lipidic driven processes as the …
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Effects of Linoleic Acid on Tether Formation between Monocytes and Endothelial Cells
… adhesion energy through the hydrolysis of PIP2 by phospholipase C.</p><p>The results of the study indicated that linoleic acid promoted membrane tether formation by increasing E-selectin bond formation and reducing the adhesion energy between the U937 plasma membrane and the actin …
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Engineered Cytoskeletal Arrays Reveal Mechanisms Of Membrane Transport And Tubulation
… a PtdIns(4,5)P2-rich lipid composition (PIP2-GUVs) tend to deform at actin/microtubule intersections along the microtubule, yet, the BAR domain protein endophilin is necessary for robust tubulation. Alternatively, in the presence of a physiological lipid mixture (LM-GUVs), kinesin-1 can …
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Probing the regulatory mechanisms of the actomyosin system in motile cells
… Peptide (PBP10) that binds preferentially to PIP2. Phosphoinositides can recruit actin-binding proteins to defined subcellular sites and alter their activity. Neutrophils, as well as developed Dictyostelium cells produce PIP3 in the plasma membrane at their leading edge in response to an …
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