Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 16 of 16 for “"PI4P"”.
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Distinct roles of PI4P 5-kinase isoforms in polar tip growth of pollen tubes
… Phosphatidylinositol-4-phosphat 5-Kinasen (PI4P 5-Kinasen) kodieren und in Pollenschläuchen exprimiert werden. Diese Kinasen wurden heterolog exprimiert und biochemisch charakterisiert, wobei die vermutete PI4P 5-Kinaseaktivität bestätigt wurde. Auf Grund ihrer Domänenstruktur können die …
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The Role of ER-Golgi Membrane Contact Sites and of FAPP1 in Phosphoinositide Homeostasis at the Golgi Complex
… species, phosphatidylinositol 4-phosphate (PI4P) turned out to be “active” in its own right as a pivotal regulator of multiple cellular functions including membrane trafficking, sphingolipid metabolism, autophagy, and cell migration. PI4P levels at the Golgi in control conditions are the …
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The Role of FAM21 in the Regulation of Mitochondrial Dynamics
… of phosphatidylinositol 4-phosphate (PI4P) trafficking, from trans-Golgi network vesicles (TGNv) and lysosomes, at the mitochondrial fission site during the last steps of the process. However, how the actin machinery is recruited and how PI4P could contribute to mitochondrial division …
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Interaction between DVAP, the orthologue of human VAPB, and Drosophila Sac1
… Sac1, a phosphoinositide-4- phosphate (PI4P) phosphatase, was identified during a genome-wide yeast twohybrid screen, as a DVAP binding partner. VAP plays a role in regulating PI4P turnover in yeast and phosphoinositides are implicated in some neurodegenerative processes. In this PhD …
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Targeting Plasma Membrane Phosphatidylserine Content to Inhibit Oncogenic Kras Function
… in the ER for phosphatidylinositol-4-phosphate (PI4P) synthesized in the PM. We show that depletion of ORP5 or ORP8 reduced PM PtdSer levels, resulting in extensive mislocalization of KRAS from the PM. Concordantly, ORP5 or ORP8 depletion significantly reduced proliferation and …
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Exploring the Impact of Endothelial Bone Morphogenetic Protein Receptor 2 Loss on Phosphoinositide and Cytoskeletal Assembly Dynamics and the Pathobiology of Pulmonary Arterial Hypertension
… an imbalance in the levels of the PI species PI4P and PI(4,5)P2. Conclusion: This study revealed abnormalities in intracellular organelles and the cytoskeleton, altered ERK phosphorylation kinetics in response to VEGF stimulation, and differential abundance of PI4P and PI(4,5)P2 in …
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Differential Regulation of Phosphatidylinositol 4-Phosphate 5 Kinase Beta during Hypertonic and Oxidative Stress
… its precursor, phosphatidylinositol 4 phosphate (PI4P). Our laboratory found that hypertonic and oxidative stress increases and decreases PIP2 levels, respectively. In this thesis, I sought to understand how these two different types of stresses alter PIP2 by examining the effects on the …
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Comparative analysis of membrane-associated signatures at mutualistic and pathogenic plant-microbe interfaces
… AM fungal structures. I also showed that while PI4P is present around intracellular AM fungal structures and around invasive P. palmivora hyphae, it is excluded from around haustoria. However, PI4P localises around haustoria in AM-colonised roots, suggesting dynamic changes in membrane identity …
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PROTEINi screens identify ORP9 as a novel regulator of KRAS-driven signalling
… out of the ER membrane in exchange for PI4P, which is then dephosphorylated by the ER-resident Sac1 phosphatase. TAT-OPX and TAT-HPX caused accumulation of PI4P on Rab7-positive endosomes, suggesting inhibition of ORP9-mediated lipid transfer. ORP9 knockdown reduced MEK1/2 …
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Evaluating Phosphatidylinositide as Lipid Biomarkers for Hyperglycemia-related Triple-negative Breast Cancer Aggressiveness
… to cell signaling in TNBC, this work identifies PI4P, PI(4,5)P2 and PIP3 lipids as potential biomarkers and points toward new strategies for risk stratification and therapeutic intervention.
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The Arf GTPase exchange factor Sec7p interaction network:
… regulation and phosphatidylinositol 4-phosphate (PI4P) metabolism are important factors for Golgi function. Here, we show that the yeast PI4-kinase, Pik1p binds specifically to Sec7p but not Gea1p or Gea2p. Taken together, the physical interaction, the colocalization and similar transport …
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Escherichia Coli as a New Platform for the Study of Phosphoinositides
… inositol, phosphatidyl inositol (4) phosphate (PI4P), and phosphatidyl inositol (4,5) diphosphate (PIP2), and is easily controlled trough the addition of inositol to the growth media of the bacterium. As an example application of my system, I use it to confirm the role of PIP2 binding in the …
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Role of diacylglycerol in the cellular response to lysophosphatidylcholine analogues
… In addition, a putative lipase Ydr444w and the PI4P phosphatase Sac1 were also identified as novel regulators of DAG homeostasis. An up-to-date map of the spatiotemporal distribution of the cytosolic pools of DAG in yeast emerged from this body of work.
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Protein Regulators of Phosphoinositides as Promoters of Cancer Metastasis
… binds to phosphatidyl inositol 4-phosphate (PI4P) in the Golgi. Through this binding, PITPNC1 recruits RAB1B to the Golgi, which in turn recruits GOLPH3. GOLPH3 facilitates elongation of the Golgi structure, enhancing vesicular release. Through this pathway, PITPNC1 increases the secretion of …
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The PI5P 4-kinase ortholog PPK-2 of Caenorhabditis elegans acts in synaptic transmission and neuronal membrane trafficking
… Bei Co-Expression der PM-assoziierten PI4P 5-Kinase PPK-1 lokalisierte PPK-2 allerdings nicht mehr ausschließlich an Endomembranen, sondern wurde auch an der PM nachgewiesen. Beide gain-of-function-Allele kodieren für mutierte PPK-2-Proteine und konnten in C. elegans-Neuronen exprimiert …
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Role of trans-Golgi network vesicles in mitochondrial fusion
… isoforms from phosphatidylinositol 4 phosphate (PI4P). Interestingly, we showed that loss of PI3KIIs resulted in mitochondrial morphology changes characterized by mitochondrial fragmentation. Mechanistic studies revealed that cells lacking PI3KIIs exhibited impaired mitochondrial fusion and were …