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Showing 1 to 16 of 16 for “"PHOSPHODIESTERASES"”.
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Regulation of cAMP Signalling to Phospholemman by Phosphodiesterases
… There is increasing evidence to suggest that phosphodiesterases (PDEs), a class of enzymes that selectively hydrolyses cyclic nucleotides, play a key role in determining cAMP signalling specificity to a number of PKA substrates in the heart. Phospholemman (PLM) is a phosphoprotein that is …
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The Role of Phosphodiesterases in Cyclic Nucleotide Compartmentation Across Different Pathways in the Adult Rat Ventricular Myocyte
… which has been postulated to be mediated by phosphodiesterases (PDEs). Research in this field has focused on compartmentation using β-adrenergic stimulation. As an extension of this work, we investigated the effects of two agonists, prostaglandin E2 (PGE2; 10 nM) and forskolin (FSK; 30 nM), …
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Physical and Functional Coupling of CFTR and PDE3A
… understanding the regulation of CFTR function by phosphodiesterases.</p>
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The Effect of Phosphodiesterase Inhibitors on the Induction of Gene Expression by Long-Acting beta2-Adrenoceptor Agonists and Glucocorticoids
Recently, phosphodiesterases (PDE)4 inhibitors have received approval as a complementary anti-inflammatory treatment for chronic obstructive pulmonary disease (COPD) patients who are already taking long-acting β2-agonists (LABA)/inhaled corticosteroid (ICS) combination therapy. However, this …
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Mrp4 Is a Crucial Regulator of Testosterone Biosynthesis
… with a primary focus on cAMP regulation by phosphodiesterases (PDEs). Continued assessment of our KO animals revealed pre-pubertal animals had reduced systemic testosterone concentrations while adult mice had normal circulating levels. The difference is pre-pubertal KO mice have increased …
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Ca2+-Activated K+ Channels in Urinary Bladder Smooth Muscle: Functional Role and Potential as Therapeutic Targets for Overactive Bladder
… we tested the hypothesis whether inhibition of phosphodiesterases (PDEs), which hydrolyze cAMP, can reduce guinea pig DSM contractility by increasing BK channel activity. Inhibition of PDEs by 3-isobutyl-1-methylxanthine (IBMX), a non-selective PDEs inhibitor, significantly reduced DSM myogenic …
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Phosphodiesterase 1 (PDE1) at the Crossroads of Calcium and Cyclic Nucleotide Signaling in Diabetic Nephropathy
… mediated by cyclic nucleotides (cAMP and cGMP), phosphodiesterases (PDEs) lead to cyclic nucleotide degradation. Our investigation focused on the role of calcium/calmodulin activated PDE1 in DKD. Three isoforms of PDE1 are differentially expressed in vascular smooth muscle cells, renal tubular …
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Functional characterization of Phosphodiesterase-4A in behavior and obesity
… has been reached with therapeutics for AD. Phosphodiesterases (PDEs) may be a potential novel target for future therapeutics aimed at combatting these psychiatric illnesses and neurodegenerative diseases. PDEs are a "super family" of 11 smaller enzyme families expressed throughout the body …
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MEK1 binds βarrestin1 directly, influencing both its phosphorylation by ERK and the timing of its isoprenaline-stimulated internalization.
… cAMP is through degradation via cyclic-phosphodiesterases (PDEs). The PDEs, especially PDE4s, are involved in many diseases including asthma, chronic obstructive pulmonary disease (COPD) and depression. Therefore, PDEs have been a consistently popular research subject for decades and …
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The Molecular Correlates of Sleep and Sleep Deprivation in vivo and in vitro
… deprivation, whilst the abundance of several phosphodiesterases are acutely increased following sleep deprivation. Metabolomic analyses of sleep deprived mouse cortex identified 3 molecular species whose abundance profile implicate them as sleep homeostats. Finally, we also set out to develop …
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Putative phosphodiesterase inhibitors as potential new chemotherapies against African Trypanosomiasis
… pathway. Identifying a compound that inhibits phosphodiesterases in trypanosomes and elevates cAMP concentrations, along with the generation of a PDE inhibitor-resistant cell line will allow more detailed examination of all aspects of the cAMP signalling pathway in T. brucei and across the …
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Modulation of cyclic adenosine monophosphate (cAMP) signalling and its therapeutic potential: pharmacological characterisation of PDE inhibitors
… explored were elevation of cAMP level through phosphodiesterases (PDEs) inhibition, adenylyl cyclase (AC) activation, as well as modulation via β-adrenoceptor (β-AR) and G proteins. A series of compounds were evaluated applying various assaying techniques and pharmacological tools using cAMP …
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Trace phosphodiesterase contamination of commercially prepared E. coli alkaline phosphatase
This document only includes an excerpt of the corresponding thesis or dissertation. To request a digital scan of the full text, please contact the Ruth Lilly Medical Library's Interlibrary Loan Department (rlmlill@iu.edu).
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Signalling to intracellular Ca2+ channels
… signals. Inhibition of cyclic nucleotide phosphodiesterases with IBMX had no effect on the Ca$^{2+}$ signals evoked by carbachol after acute stimulation with PTH(1-34). However, during sustained stimulation (60 min), when cAMP levels were increased ~15-fold by IBMX, carbachol-evoked …
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Crosstalk Signaling Between cAMP and Calcium on the Leading Edge of Migrating Human Arterial Vascular Smooth Muscle Cells
Vascular smooth muscle cells (VSMCs) are phenotypically plastic. Thus, while the VSMCs in healthy blood vessels are ‘contractile’ and control vascular tone, these cells can increase their synthetic, migratory and proliferative capacities in response to vessel injury. While largely adaptive, actions …
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ΦΩΣΦΟΔΙΕΣΤΕΡΟΛΥΤΙΚΗ ΔΡΑΣΗ ΣΕ ΚΥΤΤΑΡΟΠΛΑΣΜΑ ΣΥΚΩΤΙΟΥ ΠΟΝΤΙΚΟΥ C3HB. ΚΑΘΑΡΙΣΜΟΣ ΚΑΙ ΜΕΛΕΤΗ ΙΔΙΟΤΗΤΩΝ ΤΕΣΣΑΡΩΝ ΕΝΖΥΜΩΝ
THE ACID RIBONUCLEOLYTIC ACTIVITY OF MOUSE LIVER CYTOPLASM IS DUE TO 2 RIBONUCLEASES. THEY ARE LOW MOLECULAR WEIGHT ENZYMES THAT RESIST ACID CONDITIONS BUT ARE DESTROYED AT 100 K FOR 10 MIN . THE ENZYMES SHOW DIFFERENT PRETENCES TOWARDS END-LABELLED NATURAL SUBSTRATES (55 RNA). RNASE I SHOWS THE …