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Showing 1 to 12 of 12 for “"PGK"”.
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Protein folding in crowded environments and living cells
… of the metabolic enzyme phosphoglycerate kinase (PGK) in U2OS (human osteosarcoma) cells and observe variations in folding kinetics within a single cell. FReI experiments carried out on an ensemble of 30 cells reveal large variations in folding time from cell to cell, which are analyzed to …
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Millisecond and Submillisecond Folding Kinetics of Horse Apomyoglobin and Yeast Phosphoglycerate Kinase
… two tryptophans located in the C-terminus. PGK readily cold denatures yielding a state generally devoid of stable secondary structure. Kinetic measurements indicated the molecule collapses to a molten globule type state on the sub-ms time scale. Measurements on the wild-type protein and a …
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Multi-domain Protein Unfolding Pathway Studies by Single Molecule Techniques
… proteins – yeast phosphoglycerate kinase (PGK) and its E.coli homolog. I found that yeast PGK has much higher mechanical stability than E.coli PGK although previous literature reported that they had similar thermodynamics stability determined by bulk measurements. In collaboration with Mr. …
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In vitro and in vivo protein folding under stress
… singly-labeled, and a doubly-labeled enzyme PGK (phosphoglycerate kinase). Two mechanisms for non-additivity were proposed. In the “quinary interaction” mechanism, two tags interact transiently with one another, relieving the host protein from unfavorable tag–protein interactions. In the …
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Phase Phenomena of Proteins in Living Matter
… by modeling the protein phosphoglycerate kinase (PGK) on the temperature ($T$), pressure ($P$), and crowding volume-fraction ($\phi$) phase diagram, we demonstrate a critical transition where phases merge, and PGK exhibits large structural fluctuations. Above the critical temperature ($T_c$), the …
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Protein folding and diffusion: from in vitro to live cells
… a mutant of the enzyme phosphoglycerate kinase (PGK) in living cells as a function of temperature. To facilitate measurement in individual living cells, we developed a rapid laser temperature stepping method capable of measuring complete thermal melts and kinetic traces in about two min. We …
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The role of Xist RNA in the maintenance of X chromosome inactivation
… chromosomes also transcribed X-linked genes Pgk-i and Hprt, in a small percent of cells Xist expression led to X chromosome inactivation. The proportion of chromosome expressing X-linked genes declined, and occasionally the X became late replicating, indicating that X-inactivation can be …
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A Study of the Effects of Polyethylene Glycol on Interactions Between Glycolytic Enzymes and F-Actin, and Among Glycolytic Enzymes
… ATP:3-phospho-D-glycerate 1-phosphotransferase (PGK, EC 2.7.2.3), 2-phospho-D-glycerate hydro-lyase (enolase, EC 4.2.1.11), D-fructose-1,6-bisphosphate D-glyceraldehyde-3-phosphate lyase (aldolase, 4.1.2.13), D-glyceraldehyde-3-phosphate:NAD$\sp{+}$ oxidoreductase (GAPDH, EC 5.3.1.1), …
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IN VIVO IMAGING OF STEM CELL MEDIATED TREATMENT IN A MOUSE MODEL OF SPINAL CORD INJURY
… a viral vector expressing Luciferase under a PGK constitutive promoter (PLW vector). Results: the iron content/cell increased in proportion to the incubation time and to the iron concentration in the medium and in relation to different carriers (PLL, PB and PS) in labelled mNSCs. Longer …
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Protein folding in living cells and under pressure
… stability of yeast phosphoglycerate kinase (PGK-FRET) are cell cycle-dependent, a process strictly regulated in time, suggesting that the interplay between the intracellular environment and proteins may impact their function. In chapter 6, a new probe to study protein folding in the cell is …
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Edelgase als Tracer für Wechselwirkungen von Krusten- und Mantelfluiden mit diamantführenden Gesteinen des östlichen Baltischen Schildes
… aus Poria Guba, separiert aus dem Kimberlit PGK 12a, ergibt ein Alter von 396 Ma, ebenfalls in guter Übereinstimmung mit Rb-Sr-und Sm-Nd-Altern (ca. 400 Ma, Lokhov, pers. Mitteilung). K-Ar-Altersbestimmungen an Gesamtgestein aus Poria Guba erbrachten kein schlüssiges Alter. Die Rb-Sr- und …
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Human Embryonic Stem Cells - Constitutive Gene Expression and Differentiation Towards Definitive Endoderm and Posterior Foregut Endoderm
… These data suggested ACTB, EF1α and PGK promoters as the most stable promoters during long term culture of undifferentiated hESCs. During EB differentiation, activities of all five promoters were downregulated and EF1α was the most stable promoter although it was downregulated in 50% …