Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 5 of 5 for “"PGCLC"”.
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Mechanisms of Mouse Primordial Germ Cell Migration: The Role of SDF-1 and Cytoskeletal Dynamics
… In this work, we used PGC-like cells (PGCLCs), which are embryonic stem cell-derived analogues of migratory-stage PGCs, to investigate the modes of migration employed by mouse PGCs. To mimic the three-dimensional confinement encountered by PGCs migrating in vivo, we used flat …
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Integrin signalling in pluripotent cells acts as a gatekeeper of mouse germline entry
… mouse embryos, as well as during PGC-like cell (PGCLC) specification in both mouse and human *in vitro* stem cell models. Mechanistically, we demonstrate that β1 integrin signalling, in response to the ECM protein, laminin, blocks mouse PGCLC specification by inhibiting Wnt signalling, which …
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Genesis of the human germline
… for PGC fate and commits to PGC-like cells (PGCLCs) in response to BMP signalling. To address these questions, I performed highly-resolved transcriptomic profiling and careful epigenomic characterisation of cis-regulatory regions at key steps during the in vitro model. To determine the …
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Signalling and transcriptional regulation of human primordial germ cell specification
… in vitro derivation of human PGC-like cells (hPGCLCs). hESCs in conventional culture conditions have very low potential for germ cell fate; this increases significantly if the cells are first cultured in PGC competence-promoting media. To address how the PGC-competent state might be gained, I …
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The identification of novel factors required for the development of primordial germ cells
… in vitro system that generates PGC-like cells (PGCLCs) from ESCs to discover novel factors required for PGC development. Using this system, we have firstly shown that we can recapitulate the genetic requirement for DNA repair in PGCLCs in vitro. Secondly, we coupled this system with CRISPR/Cas9 …