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Showing 1 to 20 of 46 for “"PFGE"”.

  1. Molecular Characterization of pFGE, the Paralog of the C-α-Formylglycine-generating Enzyme

    Mariappan, Malaiyalam2012

    … Deuterostomia wurde ein Paralog des FGE, genannt pFGE, gefunden. Das humane pFGE ist zu 62,1% homolog und zu 47,1% identisch mit FGE. Seine biologische Funktion ist unbekannt. Das Ziel der vorliegenden Arbeit war, die strukturellen und funktionellen Eigenschaften von pFGE zu untersuchen. pFGE …

    goettingen Repository record

  2. Genetic Analysis of Clostridium Acetobutylicum Using Two-Dimensional PFGE to Construct a Macrorestriction Map of ATCC 824 and Restriction Enzyme Profiles for Strain Analysis

    Broussard, Suzanne ReneUniversity of Illinois at Urbana-Champaign2014

    … enzymes by one-dimensional and two-dimensional PFGE. C. acetobutylicum strains were placed into three groups based on their restriction enzyme profiles following digestion with SalI, SmaI, and MluI. C. acetobutylicum strains ATCC 824, NCIB 8052, and NRRL B-591 were grouped together. A second …

    uiuc Repository record

  3. A Genotypic Comparison of Staphylococcus lugdunensis Clinical Isolates of Diverse Geographic Origin

    Fincham, Megan A.Creighton University2001

    … specifically, pulsed-field gel electrophoresis (PFGE), arbitrarily primed polymerase chain reaction (AP-PCR), inter repetitive sequence polymerase chain reaction (REP-PCR), and southern hybridization.|PFGE together with southern hybridization data proved superior to both types of PCR based …

    creighton Repository record

  4. Pulsed-field gel electrophoresis subtyping of salmonella isolates from carcasses, lymph nodes and fecal samples of cattle from slaughter facilities in Mexico

    Ayala, Diana I.2013

    … relationship between particular serotypes and PFGE patterns, 95 isolates from a Salmonella stock culture collection of the Food Microbiology Laboratory at Texas Tech University were analyzed; isolates were originally isolated from samples collected in Veracruz, Mexico in 2009. In addition, 33 …

    tdl Repository record

  5. GENOMIC DIVERSITY IN Staphylococcus Aureus

    Barton, Bret Max

    … subjected to pulsed-field gel electrophoresis (PFGE). Thirty-nine distinct genomic macrorestriction patterns (GPs) were identified using <em>Sma</em>I; 29 of these patterns are from methicillin-resistant <em>S. aureus</em> (MRSA).</p> <p>A dendrogram showing percent similarity among the patterns …

    loma-linda Repository record

  6. Untersuchung möglicher Zusammenhänge zwischen Phänotyp, Zellwandkomposition und Genotyp in dem humanpathogenen Hefepilz Candida glabrata

    Schwarz, Alexander2012

    … genotypisch mittels Pulsfeld-Gelelktrophorese (PFGE) und MLST, phänotypisch durch standardisierte Mikrodilutionstests auf Zellwand-destabilisierende und antimykotisch wirksame Substanzen und Zellwand-biochemisch, durch die quantitative Analyse des Chitin- und Glukangehalts mittels …

    goettingen Repository record

  7. Die Verbreitung der Yersiniose beim Menschen im Großraum München unter Berücksichtigung von Schweinefleisch und Schlachtnebenprodukten als mögliche Infektionsquelle

    Zechner, KatjaLudwig-Maximilians-Universität

    … Yersinia strains were characterised with PFGE. The human strains were isolated during the period from November 2001 to June 2002. The non-human strains were isolated between 1999 and 2002. Most (62/69) of these strains were from porcine origin. In total, 41 genotypes were obtained when the …

    lmu-germany Repository record

  8. Διερεύνηση λοιμώξεων από πηκτάση-αρνητικά στελέχη του γένους Staphylococcus σε ασθενείς με προσθετικά υλικά

    Γιορμέζης, Νικόλαος2015

    … έδειξε μεγάλη γενετική ποικιλομορφία, με 67 PFGE τύπους, μεταξύ των οποίων δύο κύριοι τύποι (a, b) με 50 και 36 στελέχη αντίστοιχα. Έλεγχος με MLST ανέδειξε τρεις κύριους κλώνους (ST2, ST5 και ST16) που ανήκαν στο ίδιο κλωνικό σύμπλεγμα (Clonal Complex 2). Τα στελέχη του PFGE τύπου a …

    patras-thes Repository record

  9. Studies of the nature of Burkholderia cepacia in cystic fibrosis

    Baxter, Ian A.Aston University1996

    … chromosomal DNA by pulsed-gel electrophoresis (PFGE) (with the use of two separate restriction enzymes) and outer membrane protein (OMP) profiles. This revealed isolates of the UK CF epidemic strain to form a distinct group with a specific OMP profile. Cluster analysis of PFGE and FAME profiles …

    aston Repository record

  10. The prevention and diagnosis of central venous catheter-related infection

    Casey, Anna L.Aston University2004

    … by pulsed-field gel electrophoresis (PFGE). The genetic heterogeneity of CoNS isolated from the skin was demonstrated and specific genotypes implicated in catheter colonisation. In addition, phenotypic and genotypic typing techniques were assessed for their ability to characterise …

    aston Repository record

  11. Longitudinal study of Salmonella enterica, Escherichia coli O157:H7, and Listeria monocytogenes in a small and very small fresh meat processing plant environment

    Orellana, Eva Danira Borjas, author, Bunning, Marisa, advisor, Nightingale, Kendra, committee member, Kendall, Patricia, committee memberColorado State University. Libraries2012

    … pathogen isolates by molecular subtyping (e.g. PFGE, ribotyping) was performed to gain insight in contamination transmission within the facilities. S. enterica was isolated from 15 (4.5%) and 8 (2.4%) samples from Plant 1 and Plant 2, respectively. Characterization by PFGE using XbaI generated 6 …

    colostate Repository record

  12. Analysis of the S. pombe sister chromatid cohesin subunit in response to DNA damage agents during mitosis.

    Bhatti, SaeedaUniversity of Glasgow2008

    … integrity by pulse-field gel electrophoresis (PFGE). The northern blot experiments indicated that rad21+ expression levels increased with higher concentrations of MMS, and cell viability decreased at concentrations above 0.1%. The induction of rad21+ transcription after addition of MMS was also …

    glasgow Repository record

  13. Molecular Studies of Restriction Genes in Enteric Bacteria

    Rutebuka, Obed B.

    … technique, pulsed field gel electrophoresis (PFGE).</p> <p>The KpnAI and KpnBI R-M systems were recognized in <em>K. pneumoniae</em> strain M5al and GM236, respectively. A macro-restriction map of <em>Klebsiella pneumoniae</em> GM236 was constructed in this study using PFGE and Southern …

    loma-linda Repository record

  14. Análisis genómico y molecular de levaduras vínicas. Aplicación a la mejora del proceso de fermentación de vinos mediante selección de levaduras autóctonas

    Rodríguez Jiménez, María Esther2007

    … molecular de Electroforesis en Campo Pulsante (PFGE), y tener así un con ocimiento más amplio de las cepas de levaduras más representativas del proceso. Tras este estudio se pre-selecionaron cuatro cepas perteneciente a la especie Saccharomyces cerevisiae de entre aquéllas que mostraron los …

    cadiz Repository record

  15. Diversidad de Beta-lactamasas de espectro extendido y de líneas genéticas en aislados de Escherichia coli de diferentes ecosistemas en La Rioja

    Somalo, SergioUniversidad de La Rioja (España)2013

    … las cuales presentaron el mismo patrón de PFGE. Se analizó asimismo muestras fecales de 126 perros de la perrera municipal de la Rioja, detectándose E. coli BLEE+ en el 16 % de las mismas. Las variantes de BLEEs mas frecuentemente detectadas fueron SHV-12 (40 %), CTX-M-14 (35 %, variantes a …

    dialnet Repository record

  16. Conjugation of extrachromosomal replicons of Rhodococcus erythropolis AN12

    Yang, Joyce Chun-YiMassachusetts Institute of Technology2006

    … they encode. Pulsed-field gel electrophoresis (PFGE) revealed three previously uncharacterized megaplasmids in the genome of Rhodococcus erythropolis AN12. These megaplasmids, pREA400, pREA250 and pREA100, migrate at approximately 400 kb, 250 kb and 100 kb, respectively. Genetic screening of an …

    mit Repository record

  17. Virulence potential of enterobacteriaceae isolated from neonatal enternal feeding tubes

    Alkeskas, AANottingham Trent University2013

    … printing, via pulsed-field gel electrophoresis (PFGE), to determine if the same strains were isolated on different occasions from the NICUs. Therefore indicating whether certain strains have colonised the NICUs leading to increased exposure and risk to the neonates.

    nott-trent Repository record

  18. Genetic and phenotypic diversity of bloodstream associated Staphylococcus epidermidis isolates obtained from private and public hospitals in South Africa

    University of Pretoria2023

    … (SCCmec), pulsed field gel electrophoresis (PFGE) and whole genome sequencing (WGS). All 161 isolates (98 from private and 63 from public hospitals) were identified as S. epidermidis, with 91.9% (148/161) of the isolates being resistant to methicillin (i.e MRSE). The high prevalence of the …

    pretoria Repository record

  19. Caracterização fenotípica e molecular de isolados de Listeria monocytogenes obtidos em uma planta de processamento de carne bovina

    Camargo, Anderson CarlosUniversidade Federal de Viçosa2013

    … atípicos, eletroforese em gel em campo pulsado (PFGE), detecção de genes de virulência (inlA, inlB, inlC, inlJ, plcA, hlyA, actA e iap) e testes de resistência a antimicrobianos. Listeria spp foi identificada em 61 (22.4%) das amostras analisadas, L. monocytogenes em 23 (8.5%), L. innocua em 46 …

    brazil-ufv Repository record

  20. Genomics and host response of S. Typhi infections

    Maes, MailisUniversity of Cambridge2021

    … Typhi, based on pulse field gel electrophoresis (PFGE) patterns of S. Typhi DNA using isolates collected throughout the country between 2012 and 2015. This is followed by further analysis of a second data set using isolates collected between 1997 and 2018. Here, PFGE results were compared to whole …

    cambridge Repository record

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