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Showing 1 to 20 of 22 for “"PEpC"”.
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Expression rekombinanter pflanzlicher Phosphoenolpyruvat-Carboxylasen zur Beeinflussung des Kohlenstoffmetabolismus von Solanum tuberosum
Phosphoenolpyruvate carboxylase (PEPC) is a ubiquitous cytosolic enzyme in plants. Besides several housekeeping functions in plant metabolism, PEPC plays a cardinal role in the initial fixation of CO2 in C4 and CAM plants. The goal of this work was to regenerate transgenic C3-plants (Solanum …
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Einfluss von Histonmodifikationen auf die gewebespezifische Transkription C 4-spezifischer Gene in Mais (Zea mays)
… Recent studies that focused on the C4-specific Pepc-gene revealed a complex promoter regulation due to histonmethylation and -acetylation influenced by specific stimuli. Recent results for the C4-specific Pepc-gene revealed that specific histone acetylation and methylation sites are controlled …
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Beitrag der Histonacetylierung zur Signalintegration auf dem C 4-spezifischen Phosphoenolpyruvat-carboxylase Gen in Mais (Zea mays L.)
… phosphoenolpyruvate carboxylase gene (C4-Pepc) in maize (Zea mays L.) is regulated by multiple internal and external stimuli. C4-Pepc gene expression is restricted to mesophyll cells within foliar leaves but its activity is further induced by light and modulated by the availability of …
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Die C 4 -spezifische Phosphoenolpyruvatcarboxylase in Zea mays L.: Modellsystem zur Signaintegration auf Chromatinebene in Pflanzen
… isoform of phosphoenolpyruvate carboxylase (C4-Pepc) and have to be integrated into a single promoter response. Therefore, C4-Pepc is a perfect candidate to investigate signal integration on the chromatin level. Previous studies in our group already revealed a model for this process: The …
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Phosphoenolpyruvat-Carboxylasen in Solanum tuberosum : Charakterisierung und Expression
… by the enzyme phosphoenolpyruvate-carboxylase (PEPC). This thesis presents research into increasing this enzyme’s activity in S.tuberosum. Two clones carrying the stppc1 or the stppc2 gene were isolated from a S.tuberosum genomic phage bank by plaque and Southern hybridisation. The inserts of …
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Die epigenetische Regulation des C 4 -Syndroms : belichtungsabhängige Chromatinveränderungen am Promotor der Phosphoenolpyruvat Carboxylase aus Mais (Zea mays L.)
… controlled by phosphoenolpyruvate carboxylase (PEPC). The enzyme catalyses the formation of oxaloacetate from bicarbonate and phosphoenolpyruvate (PEP). In maize, the reaction product is rapidly converted to malate and transported into the bundle sheath where it is decarboxylated to pyruvate. …
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Structure-Function Studies on Two Phosphoenolpyruvate Carboxylases
Phosphoenolpyruvate carboxykinase (PEPCK) and phosphoenolpyruvate carboxylase (Pepc) are two important CO₂-fixation enzymes which share a similar reaction mechanism. Both operate through a lid-gated active site and have a hypothesized enol-pyruvate intermediate in their catalytic pathway. While …
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Application of metabolic and biochemical engineering techniques to enhance metabolic flux throughput and improve succinate production in Escherichia coli
… of the phosphoenolpyruvate carboxylase (PEPC) enzyme system coupled with pathway manipulations and use of different carbon sources. Incorporation of the glcU gene from Staphylococcus xylosus encoding a novel glucose uptake protein resulted in enhancement of metabolic flux throughput in E. …
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Measuring the Effects of a Step Change in the EPC Process
… The result was a step change entitled PEpC (Procure, Engineer, procure, and Construct). This research also sought to provide implementation guidelines for the recommended step change. Through an examination of the literature, a survey of industry experts, and the review of four case …
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Epigenetische Mechanismen zur Regulation der Transkription C 4-typischer Gene in Mais (Zea mays L.)
… this study, the transcriptional activation of C4-PEPC, C4-ME, C4-PPDK and the small subunit of RubisCO RbcS2 was determined by Real-Time-PCR. Histone methylation was analysed via ChIP-analysis and DNA methylation patterns were measured with restriction enzymes sensitive or insensitive to cytosine …
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Influencia de la asimilación de nitrógeno y el estrés salino sobre la capacidad fotosintética en cebada
… y disminuye la sensibilidad a malato de la pepc, indicando un aumento en la capacidad fotosintética por estimulación del flujo de carbono hacia la vía glucolítica. 3) el cultivo en amonio o nitrato amonico como fuente de n aumenta la fijación de co2, respecto al cultivo con nitrato. Las …
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Water relations, carbon fixation and growth rates of two leaf succulent species under different water regimes
… which indicated the activity of RuBPc and PEPc. R.carolii had higher photosynthetic and growth rates than R.multiflora under optimal conditions. This explained the dominance of R.carolii on the South facing, protected slope. Under moderate water stress, the species behaved similarly. There …
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Biophysical Studies of Cholesterol in Unsaturated Phospholipid Model Membranes
… ]palmitoyl-2-eicosapentaenoylphosphatidylcholine (PEPC-d<sub>31</sub>) and 1-[<sup>2</sup>H<sub>31</sub>]palmitoyl-2-docosahexaenoylphosphatidylcholine (PDPC-d<sub>31</sub>) in membranes with sphingomyelin (SM) and chol (1:1:1 mol) was compared by solid-state <sup>2</sup>H NMR spectroscopy. …
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Identifizierung und Analyse von Maismutanten mit Defekten im C 4-Zyklus
… for the induction of the transcription of Pepc, the key enzyme of the C4-photosynthesis. For Dof1 a light induced expression with an exceptional fast reaction to the light stimulus was observed. The parameters of the nitrogen and carbon metabolism are unchanged in comparison to wildtype …
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Einfluss der Wandtemperatur auf die Stoß-Grenzschicht-Wechselwirkung an einer Rampe im Hyperschall
… for the induction of the transcription of Pepc, the key enzyme of the C4-photosynthesis. For Dof1 a light induced expression with an exceptional fast reaction to the light stimulus was observed. The parameters of the nitrogen and carbon metabolism are unchanged in comparison to wildtype …
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Metabolic network design and engineering in Escherichia coli
… pantothenate kinase to enhance the activity of PEPC and PYC in order to improve succinate production. Novel metabolic networks were designed and constructed to enable E. coli to produce succinate as a product under complete aerobic conditions. Since this is naturally not possible, extensive …
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Physiology Characterization of Nitrogen Use in Maize: Opportunities for Improvement
… within each specific objective suggest that: (1) PEPc may serve as a storage sink for excess leaf N, and that a better allocation of N into Rubisco could improve maize photosynthesis, (2) No hybrid (commercial or experimental) was optimized for both N uptake and N utilization which suggests room …
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Systems analysis of Crassulacean acid metabolism (CAM) physiology and molecular biology
… regulators of key CAM genes, for example, PEPCarboxylase (PEPC), PEPCkinase (PPCK), pyruvate orthophosphate dikinase (PPDK), and pyruvate orthophosphate dikinase regulatory protein (PPDK-RP). Overall, the Gene Regulatory Network Inference provided the ranking of the potential …
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Metabolic engineering aimed at the production of keto acids from glycerol : an industrial by-product
… (PC) and phosphoenolpyruvate carboxylase (PEPC) that were analyzed by blue-native polyacrylamide gel electrophoresis (BN-PAGE) and high performance liquid chromatography (HPLC). The high-energy phosphoenolpyruvate (PEP) is then converted into ATP and pyruvate, a process mediated by pyruvate …
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