Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 16 of 16 for “"PERFUSION BIOREACTOR"”.
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Development and operation of a perfusion bioreactor for the cultivation of mammalian cells inside a sponge-like ceramic matrix
… Arbeit beschreibt Entwicklung und Betrieb eines Perfusionsbioreaktors, in dem adhärent wachsende Zellen in einer porösen Aluminiumoxid-Keramik kultiviert werden. In zylinderförmigen Gerüsten mit 10 mm Durchmesser und 5 mm Höhe konnte das Wachstum von CHO-K1, A549, MDCK und primären humanen …
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Continuous Antibody Titer Assessment of the Biomanufacturing Process using Nanofluidic Binding Assays
… are presently utilized to maintain long-term perfusion culture. However, this increases the risk of introducing external contaminants into the cell culture and the analyzed analytes. Sensors used in in-line probe-based approaches must withstand harsh clean-in-place and sterilization-in-place …
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Bioluminescence Imaging Strategies for Tissue Engineering Applications
… of stem cells in biocompatible scaffolds in a bioreactor is a promising method for creating functional engineered tissue replacements suitable for implantation. Basic studies have shown that mechanical, chemical, and pharmaceutical stimuli enhance biological functionality of the replacement as …
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Controlling Tissue Matrix Assembly of Human Mesenchymal Stem Cells toward Engineering Native-like Bone, Cartilage, and Osteochondral Grafts
… A radically novel, imaging-compatible perfusion bioreactor was designed to enhance tissue integration and spatial regulation of supplements to direct stem cell differentiation into chondrogenic and osteogenic lineages and the formation of complete osteochondral constructs. …
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Recycling and Regeneration of Spent Perfusion Media via Ion Concentration Polarization
… environmental impact of cell culture media in perfusion mammalian cell culture. We demonstrate a microfluidic separation device engineered to selectively remove metabolic waste products—specifically ammonia and lactate—from spent media using ion concentration polarization. Building on this …
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Oxygen Transport, Shear Stress, And Metabolism In Perfused Hepatocyte-Seeded Scaffolds With Radial Pore Architecture: Experimental And Computational Analyses
… while cultured in bulk 3D chitosan scaffolds and perfusion bioreactor systems. Cylindrical chitosan scaffolds with radial directed pore structures were fabricated by a thermal gradient directed from the center to the periphery. Capillary-like direct oxygenation was facilitated by embedding gas …
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Development Of Scaffold Architectures And Heterotypic Cell Systems For Hepatocyte Transplantation
… scaffolds seeded with hepatocytes in dynamic perfusion bioreactor systems. The aim was to promote cell seeding efficiency by effectively entrapping 100 million hepatocytes at high density. We found that scaffolds with radially tapering pore architecture had highly efficient cell entrapment …
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Development of a High-Throughput 3D Tumor Model for Bone Sarcomas
… polymeric scaffolds in combination with a flow perfusion bioreactor. First, we investigated the effects of flow perfusion on a 3D culture of Ewing sarcoma (ES) cells. We found that increasing levels of flow-derived shear stress promoted the secretion of insulin-like growth factor-1 (IGF-1) …
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Engineering Biodegradable Vascular Scaffolds for Congenital Heart Disease
… addition, the grafts were combined with a tissue perfusion bioreactor. The bioreactor applied fluid flow to support cell seeding, differentiation, and growth of endothelial progenitor cells on the grafts, demonstrating a robust strategy for tissue formation prior to implantation. The second …
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Bone Regeneration Potential of Mesenchymal Stromal Cells derived from a Clinically Relevant Rat Model of Osteoporosis
… the integration of cell-microcarriers into a perfusion bioreactor system was explored. Preliminary results demonstrated the feasibility of MSC growth and differentiation on microcarrier based packed beds. Moreover, AD-MSC from OP rats were unresponsive to both inductive media and shear stress …
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Modulation of the osteoblastic differentiation of marrow stromal cells for bone tissue engineering
… potential of ECM constructs generated in a flow perfusion bioreactor for different peroids of time were tested in an ectopic site in a rodent model. No osteoinduction was observed although an increase in the number of blood vessels within the implants with increasing ECM was noted. This study …
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Modeling cell proliferation in a perfusion tissue engineering bioreactor
… experiment. The experiment takes place in a bioreactor in which a cell seeded porous scaffold is placed, and the scaffold experiences a perfused flow of a nutrient-rich culture medium. The goal of the model is to assist experimentalists in evaluation of different parameter scenarios as the …
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Extracellular matrix- and pluripotent stem cell-based tissue engineering of the kidney
… This approach required the development of a perfusion bioreactor and control software, which enabled the de- and recellularization of the kidneys as well as the subsequent in vitro cultivation. Decellularization is the process of removing all cells of an organ while preserving the …
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Systems Engineering for Viral Vector Manufacturing
… reduction of scale-up. This thesis develops a perfusion bioreactor platform to produce rAAV at high cell density leveraging understandings from the mechanistic model and multi-stage transfection. The continuous process enables high cell density transfection and reduces genome titer yield per …
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A Bottom-Up Assembly Of Vascularized Bioartificial Constructs Using Ecm Based Microscale Modules
… interconnected channels that enabled long-term perfusion in-vitro and accelerated neovascularization in-vivo. A prototype, engineered liver tissue, formed by fusion of hepatocyte-containing capsules exhibited urea synthesis rates and albumin synthesis rates comparable to standard collagen …