Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 32 for “"PEGylation"”.
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DNA enzymes for tyrosine PEGylation and azido-adenylylation of peptide and protein substrates
… of a known enzyme as the starting point. PEGylation is an important artificial PTM for therapeutic peptides and proteins. PEGylation improves the pharmacokinetic properties of biopharmaceuticals by increasing circulation half time, reducing immunogenicity, increasing solubility, and …
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Design Of Functional Dendrimer Nanocarriers And Their Formulations In Propellant-Based Inhalers For Pulmonary Drug Delivery
… of PAMAM dendrimers as a function of generation, PEGylation density, PEGylation PEG length, dendrimer terminal chemistry. Our discussion is focused on how the differences in microstructures of PAMAM dendrimers will affect the function, efficacy and efficiency of PAMAM dendrimer as drug / gene …
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Hydrodynamic behavior and thermal stability of a PEGylated protein: Studies with hen egg lysozyme
… of covalent attachment of polyethylene glycol (PEGylation) on the hydrodynamic behavior and thermal stability of a model protein---Hen Egg Lysozyme (HEL). HEL was modified with a linear, 20-kD, PEG to produce mono (PEG1-HEL), di (PEG2-HEL), and triPEGylated (PEG3-HEL) species.</p><p>The …
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Formulation Devlopment Of Mesoporous Silica Nanoparticles As An Injectable Delivery System
… Investigate the effect of particle shape and PEGylation on drug delivery in hypoxic tumor cells</p> <p>3. Develop MSN capable of delivering drug/drug combinations</p> <p>To investigate the effect of surface functionalization of MSN on crystallization, loading, release and activity of …
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Cationic Glycopolymers for DNA Delivery: Cellular Internalization Mechanisms and Biological Characterization
… opportunity based on uptake mechanisms. PEGylation of PGAA polyplexes was achieved to minimize polyplex aggregation in serum. Polyplex size increased in serum, but PEGylation prevented further polyplex growth over time compared to non-PEGylated polymers. Specific targeting of hepatocytes …
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Pharmacological characterisation of selective Y4 and dual Y2/Y4 receptor agonists: novel targets for putative anti-obesity therapies
… developed and subsequently modified by either PEGylation or lipidation to improve their relatively short half-life and pharmacokinetic profile. This thesis presents a detailed study of the in vitro pharmacological data performed to aid the progression of the most promising peptide candidates. …
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Self-Assembled Gold Nanoplexes for Cancer-Targeted siRNA Delivery
… the transfection. In terms of targeted delivery, PEGylation greatly reduces the nonspecific delivery of siRNA caused by AuPEI/siRNA/PEI. However, no apparent difference is found between AuPEI/RNA/PEI-mPEG and AuPEI/RNA/PEI-PEG-FA.</p> <p>Fast fabrication, versatility, and ability for siRNA …
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Enhancement and wavelength-shifted emission of Cerenkov luminescence using multifunctional microspheres
… conjugation chemistry of functionalization and PEGylation to achieve disease targeting and to improve biodistribution of the agent. For this thesis, the microspheres were applied in several studies that were conducted in both cells and animal models. Emphasis was placed on the application of …
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Nanomaterials from lignocellulosic biomass : tailorable synthesis via surface functionalization and self-assembly mechanisms
… one-pot DES pretreatment and functionalization (PEGylation and maleilation) were demonstrated as a suitable process to prepare modified cellulose-rich pulp and lignin for nanomaterials upgrading. The resultant PEGylated cellulose-rich pulp has high cellulose content when the maleilated pulp can …
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Stealth Polymer Conjugates of Biologics
… (PEG), a technology that is colloquially termed PEGylation. PEGylated drug conjugates have a much longer plasma half-life than the native drug due to their larger size leading to slower renal clearance, reduced opsonization that reduces clearance by the reticuloendothelial system (RES) organs, …
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Zein-based nanoparticles for drug delivery applications
… cells and immune cells was then evaluated. PEGylation was shown to confer stealth effects to the zein micelles. The presence of human plasma did not impact the uptake of the micelles by melanoma cancer cells, regardless of PEG chain length. On the other hand, it decreased the uptake by …
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Adiporedoxin, an upstream modulator of endoplasmic reticulum oxidative folding and protein secretion
… ER redox chaperones. Using a cysteine-modifying PEGylation reagent, we showed Adrx oscillated between a reduced and oxidized form through the -CxxC- active site residues in response to the redox environment of the ER. Consequently, knocking down Adrx impaired the re-oxidation of protein disulfide …
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Single-Molecule Characterization of RNA via Nanopore Sensing
… such as mRNA cap structures and oligonucleotide PEGylation. Finally, I track co-transcriptional incorporation of modified nucleotides in RNA. Overall, this thesis describes a method capable of reading full-length native transcripts, uncovering structural changes arising from alternative …
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PEGylated Tissue Repair Peptide TP508 Derivatives as Second Generation Drugs for Radiomitigation and Other Systemic Applications
… polyethylene glycol moieties, a process known as PEGylation. Because TP508 is currently being evaluated as a nuclear countermeasure drug for radiomitigation, this project was initiated to evaluate several different PEGylated TP508 derivatives in an attempt to increase TP508 efficacy or decrease …
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Development of SiRNA Incorporated Surface Modified Polymeric Nanoparticle for the Potential Treatment of Global Cerebral Ischemia
… to validate surface modification achieved via PEGylation was established using UV spectroscopy. Furthermore, in vitro tests revealed that lower concentrations of nanoparticles have less toxicity to N2A cells than higher concentrations of blank nanoparticles. Higher permeability of PEG+PS-80 …
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Polymer-based Graphene Oxide Derivatives as a tunable multi-purpose platform
… pollutant model. In the field of nanomedicine, PEGylation of GO is a usual approach to improve the biocompatibility of the GO platform. The esterification reaction to bind PEG chains onto the GO platform involves different reactants and solvents, resulting in possible contamination of the …
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Visible Light-Mediated Polymerization for Protein-Polymer Conjugation: A Biocompatible Tool for Therapeutic Applications
… the late 1970s, Davis’s pioneering work on the PEGylation of proteins marked the beginning of an era of protein-polymer conjugation for the preparation of therapeutic agents. However, early methods for protein-polymer conjugation often involved complex polymer modifications, leading to low …
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Evaluation of therapeutic effect of IKK epsilon SiRNA on breast cancer cells and development of a peptide-based SiRNA delivery system
… can release siRNA under reductive condition. PEGylation of the reducible cholesteryl peptide significantly decreased nonspecific cellular uptake. Conjugation of folic acid via a PEG spacer resulted in high cellular uptake and gene silencing effect. In Chapter 6, we identified a prostate cancer …
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SYNTHESIS AND CHARACTERIZATIONS OF NANOSTRUCTURED MAGNETIC MATERIALS FOR MRI T1 CONTRAST AGENTS
… as well as promising water solubility. After the PEGylation, the nanocomposites prominently improved the colloidal stability in PBS solution. Finally the as-obtained ZMGO-PEG nanocomposites maintained the bright fluorescence and were successfully used for in vitro imaging application. Moreover, …
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Development of Dendritic Polymers as a Modular Drug Delivery Platform for Avascular Tissues
… to other avascular tissues, the impact of PEGylation on the surface charge presentation and drug delivery properties of these conjugates had not been well studied. The first part of this thesis developed an experimental technique to probe how PEG chain length and chain density impact the …
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