Global ETD Search
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Showing 1 to 20 of 21 for “"PDZ Domains"”.
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Regulation of cellular functions of PDZ domains by membrane lipids.
Regulation of cellular functions of PDZ domains by membrane lipids.
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Modulating protein-protein interactions : novel inhibitors of PDZ domains and tubulin dynamics
… is already a broadly studied area, whereas the PDZ domaininteractions is a relatively recent area of study. The literature in these areas is reviewed.This research project designed and synthesised novel molecules which were capable ofmodulating the dynamics of the tubulin dimers or the …
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Mapping the synapse : synthesis and validation of chemical probes for interrogating PDZ domains
… in the brain. These complexes consist of many PDZ domain-containing proteins. PDZ domains bind selectively to peptide sequences at the C-termini of partner proteins, and they play an essential role in the regulation of synaptic macromolecular complexes. While many PDZ domain-containing proteins …
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SCRIBBLE: A POTENTIAL DUAL KINASE INHIBITOR
… as a binding partner. Scribble contains 16 LRR domains and four PDZ domains. We performed immunoprecipitation (IP) assays and discovered ERK2 as another binding partner. Surprisingly, no interaction was observed with the highly homologous MAP kinase, ERK1. No other representative kinases showed …
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Structural characterization of the type II secretion system of Aeromonas hydrophila
… a trans-membrane helix and two periplasmic domains. The two periplasmic domains are involved in recognition and selection of protein substrates which are subsequently transported across the outer membrane and free of the cell. This study focuses exclusively on the two periplasmic domains …
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The Role of the Scaffolding Protein INAD in Localization of Signaling Complexes to the Rhabdomeres of Drosophil Photoreceptors
… InaD, a scaffolding protein containing 5 PDZ domains, organizes proteins involved in the visual signaling pathway into complexes within a microvillar stack of membranes known as the rhabdomere. Light activation of rhodopsin activates a signaling cascade via a Gq-coupled reaction that …
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Testing Whether MRP4 (a cAMP Efflux Pump) and the Beta 2 Andrenergic Receptor (an Upstream Regulator of cAMP Signaling Pathways
… second-messenger, cAMP, from cells. MRP4 has a PDZ interacting motif at its carboxy terminal end through which it binds to scaffolding proteins NHERF1 and PDZK1. Previous studies have shown that PDZK1 serves as a scaffold physically coupling MRP4 with the cystic fibrosis transmembrane …
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Specificity and structural characterization of the PDZ domain from DegS, an extracytoplasmic E. coli protease
… The C-terminal domain has limited homology to PDZ domains and was thought to be involved in regulation or substrate recognition. A model of this PDZ domain was generated from NMR solution studies and homology modeling. Peptide selection studies identified the sequence Tyr-Tyr-Phe (YYF) as a …
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Role of Phosphorylation In The Regulation of Prmt5
… of PRMT5 has a recognition motif shared by PDZ domains and 14-3-3 proteins. In order to bind to this motif, 14-3-3 proteins require the C-terminus to be phosphorylated, while PDZ domain recognition is phospho-independent. From these data, a new regulatory mechanism that affects PRMT5 …
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Protein Mechanics Through X-Ray Crystallography
… of perturbation to a series of members of the PDZ protein family. First, we show that cycles of mutagenesis coupled with X-ray crystallography can reveal an anisotropic, distributed pattern of physical interactions in a PDZ domain, PSD-95 PDZ3. This pattern is functionally important and deeply …
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Tissue specific regulation of the high density lipoprotein (HDL) receptor, scavenger receptor Class B, Type I (SR-BI) by the scaffold protein PDZK1
PDZK1 is a four PDZ-domain containing cytoplasmic adaptor protein that binds the Cterminus of the high-density lipoprotein (HDL) receptor SR-BI. Abolishing PDZK1 expression in PDZK1 knockout (KO) mice leads to a post-transcriptional, tissue-specific decrease in SR-BI protein level and an increase …
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Evolutionary Constraints Specifying Protein Folding and Function
… expression and characterization of WW domains designed with varying levels of coupling information indicates that incorporating even small amounts of coupling information has a notable impact on these proteins' ability to fold. Moreover, different coupling-based design approaches …
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Allosteric activation of periplasmic HtrA proteases
… contains a trypsin-like protease domain and a PDZ domain. In the absence of stress, autoinhibitory interactions stabilize an inactive conformation of DegS. Allosteric activation occurs as a consequence of the linked binding of unassembled outer-membrane proteins to the PDZ domains and RseA to …
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Structural Modes in Proteins: A Case Study in the PDZ Domain
… architecture in an individual protein (the third PDZ domain from rat PSD95) by measuring physical interactions between all pairs of residues. This global perturbation analysis is performed by making evolutionarily conservative mutations at every position in a protein and observing physical effects …
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A Global Experimental Analysis of Protein Function: A Case Study in the PDZ Domain
… system that measures the cellular function of a PDZ domain in a high-throughput and quantitative manner. We perform a comprehensive single amino acid mutagenesis experiment to show that most positions in the protein are robust to most mutations, and the set of positions that shows sensitivity to …
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Dynamic Scaffolding in a G-Protein Signaling Cascade
The file named "mishraprashant.pdf" is the primary dissertation file. Supplemental movie files ("Mishra_Movie_S1.mpg", "Mishra_Movie_S2.mpg") are also available.
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Mechanisms of Eph/ephrin mediated cell-cell communication
… ligands can signal by interactions with various PDZ-domain containing proteins. Using a broad range of in vitro assays the presented work demonstrates that upon binding to their cognate receptor, ephrinB ligands rapidly activate Src family kinases (SFKs) which subsequently phosphorylate ephrinB. …
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The Structural Properties of Adaptation and Allostery in Proteins: A Case Study in a PDZ Domain
… path to new specificity in a model protein PSD95pdz3 where one intermediate, a Glycine (G) to Threonine (T) mutation at position 330, removed from the binding site of the protein, maintains native function while simultaneously adapting the protein to an alternate function (termed a 'conditionally …
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Isothermal Titration Calorimetry Studies Of Protein-Mediated Interactions And Preliminary Structural Studies Of Tandem Pdz1-2 Domain Of Psd-95 Protein
… such interactions for two proteins, the PDZ domain and frataxin, involving peptide and metal binding ligands, respectively. The three component projects of this work comprised (1) thermodynamic analysis of PDZ domain binding using calorimetry; (2) X-ray crystallographic structural studies …
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Structural Basis Of Epigenetic Regulation And Protein Scaffolding In Development And Diseases
… solved the complex structures of the NHERF1 PDZ1 domain with the C-terminal sequence of CXCR2 in two different crystal forms. Although the superposition revealed a high degree of overall structural similarity, distinct conformations were observed between the two forms in substrate-binding …
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