Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 78 for “"PDZ"”.
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Activated RhoA Positively Regulates Exchange Activity of PDZ-RhoGEF
… nucleotides. Recently, our lab discovered that PDZ-RhoGEF (PRG), a member of the RGS-RhoGEFs, bound tightly to both nucleotide-free and activated RhoA (RhoA•GTP). Using deletion analysis and pulldown assays, I was able to show that this interaction occurs between the PH domain of PRG and …
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Regulation of cellular functions of PDZ domains by membrane lipids.
Regulation of cellular functions of PDZ domains by membrane lipids.
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Structural Modes in Proteins: A Case Study in the PDZ Domain
… architecture in an individual protein (the third PDZ domain from rat PSD95) by measuring physical interactions between all pairs of residues. This global perturbation analysis is performed by making evolutionarily conservative mutations at every position in a protein and observing physical effects …
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Logic and Mechanism of an Evolutionarily Conserved Interaction in PDZ Domain
… the physical basis of specificity in the PDZ domain. The co-evolutionary energetic map of the PDZ domain predicts a long range interaction between position 372, a known specificity determinant that directly interacts with ligand, and position 322. Thermodynamic measurements in one PDZ …
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Somatostatin Receptor 2 Regulation By C-Terminal Motifs and Pdz Domain Proteins
… density protein/Discs large-1/Zona occludens-1 (PDZ) domain interactions often regulate the trafficking and signaling potential of other GPCRs, we examined the role of the sst2 PDZ ligand and additional C-terminal residues in controlling its intracellular trafficking, signaling, and regulation. …
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Modulating protein-protein interactions : novel inhibitors of PDZ domains and tubulin dynamics
… is already a broadly studied area, whereas the PDZ domaininteractions is a relatively recent area of study. The literature in these areas is reviewed.This research project designed and synthesised novel molecules which were capable ofmodulating the dynamics of the tubulin dimers or the …
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Mapping the synapse : synthesis and validation of chemical probes for interrogating PDZ domains
… in the brain. These complexes consist of many PDZ domain-containing proteins. PDZ domains bind selectively to peptide sequences at the C-termini of partner proteins, and they play an essential role in the regulation of synaptic macromolecular complexes. While many PDZ domain-containing proteins …
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A Global Experimental Analysis of Protein Function: A Case Study in the PDZ Domain
… system that measures the cellular function of a PDZ domain in a high-throughput and quantitative manner. We perform a comprehensive single amino acid mutagenesis experiment to show that most positions in the protein are robust to most mutations, and the set of positions that shows sensitivity to …
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Identification de nouvelles interactions entre les protéines à domaines PDZ et les protéines kinases.
… jouant le rôle d’adaptateurs. Les domaines PDZ (PSD-95, DLG-1 et ZO1) sont des domaines d’interactions protéines-protéines impliqués dans la polarité, la signalisation et l’adressage. Ces domaines protéiques reconnaissent en général, un motif consensus en extrémité C terminale de la protéine …
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Probing peptide binding to the second PDZ domain of hPTP1E using FT-IR and MS
… were evaluated for binding to the second PDZ domain of human protein tyrosine phosphatase 1E using FT-IR and mass spectrometry. This PDZ is known to bind the C-terminal motif X-Ser/Thr-Val. MS demonstrated the formation of a 1:1 complex between PDZ and ENEQVSAV but not with peptides …
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Specificity and structural characterization of the PDZ domain from DegS, an extracytoplasmic E. coli protease
… The C-terminal domain has limited homology to PDZ domains and was thought to be involved in regulation or substrate recognition. A model of this PDZ domain was generated from NMR solution studies and homology modeling. Peptide selection studies identified the sequence Tyr-Tyr-Phe (YYF) as a …
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A Critical Role Of Cxcr2 Pdz Motif-Mediated Interactions In Endothelial Progenitor Cell Homing And Angiogenesis
… CXCR2 possesses a consensus PSD-95/DlgA/ZO-1 (PDZ) motif at its carboxyl terminus. The PDZ motif has been reported to regulate cellular signaling and functions. Here we investigated the potential role of the PDZ motif in CXCR2-mediated EPC motility and angiogenesis. We have found that …
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The Structural Properties of Adaptation and Allostery in Proteins: A Case Study in a PDZ Domain
… path to new specificity in a model protein PSD95pdz3 where one intermediate, a Glycine (G) to Threonine (T) mutation at position 330, removed from the binding site of the protein, maintains native function while simultaneously adapting the protein to an alternate function (termed a 'conditionally …
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NS1 of H5N1 Interacts with SAP-97 in a PDZ-dependent Manner to Disrupt Epithelial Barrier Integrity
… nonstructural protein 1 (NS1) contains a class I PDZ motif. The NS1 proteins of avian origin contain the PDZ motif ESEV, which has been found to bind several cellular PDZ domain proteins. The interaction of NS1 and host proteins via the PDZ motif is a determinant in the virulence of influenza …
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Role of the mGRIP1 homologue DGrip in the Drosophila neuromuscular system
… (DGrip), ein Protein bestehend aus sieben PDZ Domänen. DGrip mutante Fliegen zeigen im Wesentlichen vier Defekte: 1. den spezifischen Verlust der Antwort auf Wegweisermoleküle in einer Subgruppe embryonischer Muskeln, resultierend in einen leicht zu identifizierenden Muskelphänotyp, 2. eine …
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Testing Whether MRP4 (a cAMP Efflux Pump) and the Beta 2 Andrenergic Receptor (an Upstream Regulator of cAMP Signaling Pathways
… second-messenger, cAMP, from cells. MRP4 has a PDZ interacting motif at its carboxy terminal end through which it binds to scaffolding proteins NHERF1 and PDZK1. Previous studies have shown that PDZK1 serves as a scaffold physically coupling MRP4 with the cystic fibrosis transmembrane …
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Cxcr2 Macromolecular Complex In Pancreatic Cancer: A Potential Therapeutic Target In Tumor Growth
… CXCR2 possesses a consensus PSD-95/DlgA/ZO-1 (PDZ) motif at its carboxyl termini, which might interact with potential PDZ scaffold/adaptor proteins. We have previously reported that CXCR2 PDZ motif-mediated protein interaction is an important regulator for neutrophil functions. Here, using a …
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Protein Mechanics Through X-Ray Crystallography
… of perturbation to a series of members of the PDZ protein family. First, we show that cycles of mutagenesis coupled with X-ray crystallography can reveal an anisotropic, distributed pattern of physical interactions in a PDZ domain, PSD-95 PDZ3. This pattern is functionally important and deeply …
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Tissue specific regulation of the high density lipoprotein (HDL) receptor, scavenger receptor Class B, Type I (SR-BI) by the scaffold protein PDZK1
PDZK1 is a four PDZ-domain containing cytoplasmic adaptor protein that binds the Cterminus of the high-density lipoprotein (HDL) receptor SR-BI. Abolishing PDZK1 expression in PDZK1 knockout (KO) mice leads to a post-transcriptional, tissue-specific decrease in SR-BI protein level and an increase …
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