Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 7 of 7 for “"PCR-SSCP"”.
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Strukturelle und funktionelle Analysen von Bakterienpopulationen mit Hilfe der PCR-SSCP in sechs unterschiedlichen Modellböden
… six different model soils was investigated with PCR-SSCP. The eubacterial and actinomycetical community was analysed with a structural and the community of nitrogen fixing bacteria with a functional approach. For the first time it could be shown that it is also possible to analyse bacterial …
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The glycosylation of Dichelobacter nodosus fimbriae
… isolates using the polymerase chain reaction (PCR). Different fimA amplimers were analysed using single-strand conformational polymorphism (SSCP) and by DNA sequencing. On the basis of the PCR-SSCP pattern obtained and subsequent DNA sequencing, 16 new D. nodosus isolates belonging to eight …
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Distinção de espécies de Amphimedon Duchassaing & Michelotti, 1864 (Niphatidae, Haplosclerida, Demospongiae) da costa brasileira por estudos de diversidade genética e morfológica
… to the Single Strand Conformation Polimorphism (SSCP) technique. The individuals of Amphimedon viridis show a similar ITS 1 migration pattern, even when coming from distant collecting localities on the Brazilian Coast. The results presented support the idea that A. aff. compressa comprises …
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Identification of genetic variation in the Keratin-associated protein and KRT33A genes of the Swakara sheep of Namibia
… amplified using the Polymerase Chain Reaction (PCR). Genetic variation was assessed using PCR-Agarose gel electrophoresis in the KAP1.1 gene. As for the KAP1.3, KAP3.2 and KRT33A, genetic variation was assessed using Polymerase Chain Reaction-Single Strand Conformational Polymorphism (PCR-SSCP). …
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Mutation analysis at the lipoprotein lipase gene locus in two South African kindreds
… oligo nucleotide (ASO) hybridisation, "ARMS" PCR, PCR-SSCP, RT-PCR and DNA sequence analysis. In an earlier separate study, in vitro transfection results showed that the I194T mutant was catalytically inactive. Our findings of zero LPL activity in JJ's post-heparin plasma, implies that the …
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Associations between gene variations and milk composition in New Zealand dairy cattle: A thesis submitted in fulfilment of the requirements for the Degree of Doctor of Philosophy at Lincoln University
… - Single Stranded Conformational Polymorphism (PCR-SSCP) approach, and then a modelling approach was used to ascertain whether the genetic variation (if detected) was associated with gross milk traits (including milk volume, fat percentage and protein percentage), and milk fat composition. At …
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Gene variations of FABP4, SCD, GH, and IGF1R and their associations with meat and carcass traits in sheep : A thesis submitted in fulfilment of the requirements for the Degree of Doctor of Philosophy at Lincoln University
… IGF1R were examined. The optimised methods for PCR and SSCP were established to balance efficiency and efficacy. Higher PCR annealing temperatures improved band specificity for FABP4 and SCD genotypes. Polyacrylamide gels with 10 - 14% concentration were recommended for SSCP electrophoresis. The …