Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 47 for “"PCR-RFLP"”.
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Development and validation of PCR-RFLP assay for identification of Gambierdiscus species in the Greater Caribbean region
… provide an alternative approach, I developed a PCR-RFLP protocol for efficient, rapid, and cost-effective identification of Gambierdiscus species in the Gulf of Mexico and Caribbean Sea, where CFP cases and Gambierdiscus spp. have been reported. The assay targets the D1-D2 hypervariable regions …
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Karakterisasi genetik udang jari (Metapenaeus elegans De Man, 1907) hasil tangkapan dari Laguna Segara Anakan, Cilacap, Jawa Tengah berdasarkan haplotipe DNA mitokondria dengan menggunakan metode PCR-RFLP
… DNA mitokondria dengan menggunakan metode PCR-RFLP. Jenis penelitian ini adalah penelitian deskriptif. Sampel yang digunakan dalam penelitian ini adalah 7 individu udang jari (Metapenaeus elegans) bagian kaki jalan dan ekor yang diambil dari hasil tangkapan dari Laguna Segara Anakan, …
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Polymerase chain reaction-based genotyping of swine MHC DQB and DRB alleles
… complex DQB and DRB loci using the PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) analysis technique is described. SLA DQB and DRB locus-specific oligonucleotide primers were designed to analyze expressed genes using the reverse transcription-polymerase …
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Diversity of the butyrate-producing microflora of the human gut.
… isolates were grouped by 16S rDNA PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism) with the restriction enzyme Alu-I, which proved to give the best ribotype discrimination from a total selection of four restriction enzymes. A total of 18 Alu-I PCR-RFLP …
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Tipagem de papilomavírus humanos em amostras cervicais de pacientes coinfectadas pelo vírus da imunodeficiência humana
… dos fragmentos obtidos por enzimas de restrição (RFLP) para detecção de genótipos de HPV em amostras cervicais de citologia de 140 pacientes infectadas por HIV atendidas no Serviço de Colposcopia do Hospital dos Servidores do Estado (HSE), Rio de Janeiro, no período de 2003 a 2005. A detecção e a …
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Freqüências de variantes alélicas de genes ligados ao sistema imunológico em uma população de indivíduos de origem japonesa no sul do Brasil
… TCRBV13S5 e TCRBV18 foram analisados por PCR-RFLP. As freqüências alélicas foram determinadas e comparadas com as freqüências encontradas em outros grupos étnicos (caucasóides e afro-brasileiros). Nós observamos a ausência do alelo CCR5D32 na população testada. Os polimorfismos dos …
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Caracterização e análise filogenética molecular de novos isolados fixadores de nitrogênio
… de Comprimento Aleatório amplificados (PCR-RFLP), aplicada ao 16S rDNA e 16S-23S rDNA e sequenciamento de DNA, aplicada ao 16S rDNA. Trinta e oito isolados foram divididos em quatro grupos identificados entre os gêneros Herbaspirillum e Burkholderia e oito grupos não identificados por …
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Symbionts and immune defence in lepidopteran crop pests
… useful in studies of these pests. First, using PCR, PCR-RFLP and gene sequencing, we confirmed the presence of the Old World bollworm Helicoverpa armigera in Minas Gerais state and identified the prevalence and genetic diversity of symbionts with potential use for noctuid biocontrol such as …
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Molecular characterization of ovine GM1 gangliosidosis
… (Cys229Phe) amino acid substitution. A PCR-RFLP assay using AciI restriction enzyme was designed for genotyping lambs. To date, 1664 animals have been genotyped using the DNA-based diagnostic and all affected lambs were homozygous for this mutation. Conversely, individuals with normal …
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Candidate Gene Expression and SNP Analyses of Toxin-Induced Dilated Cardiomyopathy in the Turkey(Meleagris gallopavo)
… transcription polymerase chain reaction (RT-PCR) in toxin-induced DCM affected and unaffected turkeys. Cardiac TnT and PLN were chosen because their spontaneous expression has been reported to be associated with IDCM. We also scanned these genes for single nucleotide polymorphisms (SNPs) that …
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Molekuláris biológiai módszerek fejlesztése gluténmentesség ellenőrzésére
… alapú mérésekkel kapott mennyiségi eredményt. A PCR módszeren alapuló DNS vizsgálat nem helyettesíti a fehérje módszereket, inkább megerősítésre, ellenőrzésre szolgál, viszont elég bizonyítékot nyújt arra, hogy az adott élelmiszer valamikor búzával vagy vele keresztreagáló gabonával …
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Genetic Association Study of Osteoporotic Vertebral Compression Fractures in Postmenopausal Women
… and 117 control postmenopausal Korean women. PCR-RFLP and real-time PCR assay were used to analyze VEGF, MTHFR, and TS polymorphisms. Homocysteine levels were also measured to determine whether or not polymorphisms of the VEGF gene affect homocysteine/folate metabolism. The AA genotype of the …
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Development and application of Helicobacter pylori surveillance to improve antimicrobial treatment
… showed a novel 3’-mismatched reverse primer PCR to be the most sensitive approach. Sequence analysis of 16S rDNA in tetracycline resistant isolates identified the A926g mutation for the first time from a UK H. pylori isolate and a novel A926C mutation. The mechanism of unstable amoxicillin …
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Characterising variation in the gene for the Dichelobacter nodosus Type IV fimbrial protein: Development of a molecular typing system
… This study describes the development of a PCR specific for the variable region of the fimA gene. Cloning and sequencing of a single field derived amplimer and eight cultures currently being used in commercial footrot vaccines (Mallinckrodt Veterinary Ltd), is described. The rimA sequences …
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Virus restriction gene variants and their possible role in neurocognitive function in children born to HIV-infected mothers
… Genotyping for allelic variants was done using PCR-RFLP, SNaPshot® and Sanger DNA sequencing. Statistical analysis was carried out to determine association between genotypes, HIV status and neurocognitive function. The observation of different genetic variants or combinations of genotypes …
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Genotyping and antibiotic resistance of thermophilic Campylobacter isolates from Vietnam and Kenya
… isolated from meat were identified by multiplex PCR, and tested for the presence or absence of 29 gene loci associated with virulence, lipooligosaccharide (LOS) biosynthesis and further functions. flaA typing, multilocus sequence typing and investigation by microarray assay showed a high degree …
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Molecular Pathogenesis and Development of a Genetically Engineered Vaccine for Type-2 Porcine Circovirus
… fragment length polymorphism (PCR-RFLP) assay was developed that is capable of detecting all known PCV isolates and differentiating between infections by nonpathogenic PCV1 and pathogenic PCV2. In order to study the structural and functional relationship of PCV genes and to …
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Investigation of a new focus of Cutaneous Leishmaniasis in Ghana
… of the aspirates were inoculated onto FTA cards. PCR and PCR-RFLP were directly performed on the isolated DNA and the FTA cards. The pattern of bands produced from the patient samples were a complete deviation from DNAs of all the positive controls of Leishmania species. The sequenced PCR products …
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Genome Analysis of the Ashanti Dwarf pig of Ghana
… pig. SNPs identified were further validated with PCR-RFLP assays. Results from this study suggest high genetic diversity within the Ashanti Dwarf pig populations. Population cluster analysis performed in this study also suggests that although the African pigs (Ashanti Dwarf pig and Busia pigs) …
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