Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 21 for “"PCR-DGGE"”.
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Using Hplc and Pcr-Dgge for the Assessment of Phytoplankton and Bacterioplankton Communities in Scottish Freshwater Lakes
… and denaturing gradient gel electrophoresis (PCR-DGGE) for the assessment of the plankton community in Scottish freshwater lakes. Phytoplankton composition and biomass were determined using microscopy and HPLC, while bacterioplankton density and diversity were assessed using flow cytometry …
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Molecular microbial ecology of hospital ward environments.
… of interest. Antibiotic-resistance gene specific PCRs were used to investigate the presence of resistance determinants. PCR-DGGE was used to resolve fragments of the 16S rRNA gene from total DNA extractions and the resulting sequence data was used to identify microorganisms present. Cell viability …
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A polymerase chain reaction and denaturing gradient gel electrophoresis procedure for analysis of arbuscular mycorrhizal fungi in soil
… and denaturing gradient gel electrophoresis (PCR-DGGE) procedure for the detection of fungal 18S ribosomal RNA gene was developed with reference cultures. Five AMF species were procured from the International Culture Collection of Arbuscular and Vesicular-Arbuscular Mycorrhizal Fungi (INVAM). …
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Investigation of bacteria associated with Australian wine grapes using cultural and molecular methods
… by plate culture, enrichment culture and PCR-DGGE. B. thuringiensis carried over into wine processing but did not grow in juice or wine and did not adversely affect the growth of Saccharomyces cerevisiae or Oenococcus oeni in liquid culture. B. thuringiensis inhibited the growth of several …
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Bacterial Diversity of the Atacama Desert, Chile: The Challenges of Characterizing the Community Dynamics of Extreme Oligotrophic Ecosystems
… to extreme oligotrophic communities. Preliminary PCR-DGGE analysis of a west-east elevational transect from the Pacific Ocean near Antofagasta to the western slopes of the central Andes indicated that bacterial communities along this transect belonged to two distinct community types: 1) hyperarid …
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Detection of an Introduced Bacterial Culture in Greywater Treatment Reactors
… the 16S rRNA gene by polymerase chain reaction (PCR) and denaturing gradient gel electrophoresis (DGGE) revealed community profiles of the dominant bacterial species. Species and strainspecific primers targeting either the 16S rRNA gene or the intergemc spacer (ITS) between the 16S and 23S rRNA …
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Comparative analysis of primate hydrogenotrophic microbiota
… and SRB in various species of primates using PCR-DGGE fingerprinting targeting Archaea and Desulfovibrionales 16S rDNA, clone library construction of Archaea 16S rDNA and mcrA gene, and quantitative real-time PCR targeting mcrA and dsrA genes. Functionality of methanogens was determined by …
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Molecular characterisation of selected gastrointestinal microbiota in South African HIV-positive patients during HAART
… Bifidobacterium and Lactobacillus species using PCR-DGGE. qPCR was used to determine the abundance of total bacteria, Bifidobacterium, Lactobacillus, Escherichia coli, the Bacteroides/Prevotella, Clostridium coccoides and Clostridium leptum groups. ... In addition, three potential intestinal …
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Identification of The Causative Bacteria In Musculoskeletal Infections Using 16S Rdna - Denaturing Gradient Gel Electrophoresis Analysis
… results. The goal of this project was to use PCR amplification of a portion of the 16S rRNA gene to test an alternative approach for the identification of these pathogens and to assess the diversity of the bacteria involved. The advantages of this alternative method are that it should increase …
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Evaluación del sistema de agua de circulación de una central nuclear mediante técnicas de microbiología molecular
… y técnicas de Microbiología Molecular como PCR, DGGE y secuenciación de ADN, además de microscopía óptica y de epifluorescencia. La cantidad de microorganismos encontrados está en concordancia con la referenciada por otros autores en aguas dulces, y no muestra diferencias importantes a lo …
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Caracterização fisiológica e genética do Lactobacillus delbrueckii UFV H2b20 desprovido da capacidade de imunoestimulação
… foi realizada por análise dos perfis de PCR-DGGE. Ambas bactérias apresentaram na microscopia eletrônica de transmissão característica peculiar do H2b20, a de formar bastonete alongado quando na fase estacionária. As células do mutante mostraram-se com diâmetro maior do que as da cultura …
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Molecular ecological analysis of methanogenic communities in terrestrial and submarine permafrost deposits of Siberian Laptev Sea area
… permafrost. By polymerase chain reaction (PCR), DNA was extracted from each of the samples and analyzed by molecular microbiological methods such as PCR-DGGE, RT-PCR, and cloning. Furthermore, these samples were used for in vitro experiment and FISH. The submarine permafrost analysis of the …
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Impacts of diet inversion on voluntary intake, digesta and fecal composition, and bacterial community composition in rumen of cattle fed tropical forage-based diets
… rumen bacterial community was evaluated by PCR-DGGE in the young bulls from 6 days before to 20 days after the second diet inversion. The pattern of microbial diversity was evaluated through a clustering procedure using the unweight pair group method with arithmetic mean. Stabilization of …
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Mixing it up: Multiple symbiont acquisition strategies as an adaptive mechanism in the coral Stylophora pistillata
… using denaturing-gradient gel electrophoresis (DGGE) and real-time PCR. DGGE confirmed previous reports that in the Gulf of Eilat, Red Sea, shallow water (2-6m) <italic>S. pistillata</italic> adult coral colonies host clade A <italic>Symbiodinium</italic>, while deep-water (24-26m) colonies host …
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An Evaluation of a Yeast-Derived Mannan Oligosaccharide and Its Mode of Action in Swine
… respectively. Microbiota analysis by 16S rDNA PCR-DGGE revealed that carbadox and Bio-MosRTM made pigs more similar to each other by 1 and 3 weeks post-wean, respectively. Both in vitro and in vivo analysis of the effect of gastrointestinal environments on percent disappearance of Bio-MosRTM …
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Diversidade de bactérias endofíticas em frutos de café
… seqüenciamento direto de amplicons obtidos por PCR, enquanto que para o das não-cultiváveis foram utilizados primer específicos para grupos de bactérias de três filos, Proteobacteria classes, α, β e γ, Firmicutes e Actinobacteria, sendo os produtos do Nested-PCR analisados por eletroforese em …
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Microbial monitoring and degradation of lindane in soil
… was assessed, using methane oxidation assays and PCR-DGGE (polymerase chain reaction – denaturing gradient gel electrophoresis) respectively. Methane oxidation assays with historically polluted soils revealed that in the long-term methane oxidation was inhibited by HCH pollution. PCR-DGGE and …
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Studies on the behaviour of endocrine disrupting compounds in a membrane bioreactor
… gradient gel electrophoresis (PCR-DGGE).
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Microbial diversity and activity in temperate forest and grassland ecosystems
… by denaturing gradient gel electrophoresis (DGGE) and sequencing of excised DGGE bands. I found that functional group identity can affect AOB community structure. In particular, the presence of legumes, both in monoculture or in mixture with forbs and grasses, lead to AOB community …
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Impatto di rifaximina sul microbiota intestinale: selezione di bifidobatteri antibiotico resistenti
… molecular culture-independent techniques (FISH, PCR-DGGE). Metabolic outputs in terms of SCFA profiles were determined by HPLC analysis. Collected data demonstrated that rifaximin as well as antibiotic and probiotic treatment did not change drastically the intestinal microflora, whereas bacteria …
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