Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 9 of 9 for “"PCR real time"”.
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Arthropod-borne infections in the United Kingdom and Saudi Arabia
… prevalences from these different hosts using PCR- based molecular typing tools. Trypanosoma infections were found in mall rodents from the UK and Ireland, however the presence of the invading bank vole (Myodes glareolus) in Ireland appears to have disrupted the host-parasite relationship …
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Detection of the Oak Wilt Pathogen in Sapwood of Northern Oak Species Using PCR Technology
… involving the use of polymerase chain reaction (PCR) have the potential to offer faster and more reliable plant disease diagnostics. The purpose of this thesis was to 1) compare the use of a nested PCR and real-time PCR protocol to detect C. fagacearum in sapwood drill shavings using published …
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Regulation of Adrenal Steroidogenesis by Interleukin-6
… was extracted from the cells or tissue. Standard PCR, real time PCR, and western blot assays were used to determine the effects of IL-6 on the enzymes involved in cortisol and DHEA synthesis, steroidogenic factor-1 (SF-1), steroidogenic acute regulatory protein (StAR), and dosage sensitive sex …
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Seroprevalence and characterization of Brucella SPP. isolated from humans and cattle in Southern and Western provinces of Zambia..
… and analysed. Molecular analysis (16S rRNA PCR, Real-Time PCR, Multiplex Bruce Ladder PCR) was conducted to isolate, identify and characterize Brucella spp. The Brucella isolates were evaluated for susceptibility to six antimicrobials using the disk diffusion method, namely; rifampicin (5μg …
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Seroprevalence and characterization of Brucella SPP. isolated from humans and cattle in Southern and Western provinces of Zambia..
… and analysed. Molecular analysis (16S rRNA PCR, Real-Time PCR, Multiplex Bruce Ladder PCR) was conducted to isolate, identify and characterize Brucella spp. The Brucella isolates were evaluated for susceptibility to six antimicrobials using the disk diffusion method, namely; rifampicin (5μg …
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Molecular detection and identification of phytoplasmas in sugarcane in Hawaii, Thailand, Cuba and Near East
… et al., 1994; Wongkaew et al., 1997). Q-PCR (real-time PCR) offers the opportunity to detect the phytoplasma in a sensitive, specific and quick manner, but that is not true for sugarcane plants with a very low titer of phytoplasma. Therefore, nested-PCR is better than qPCR for low titer …
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Cloning and functional characterization of novel genes expressed preferentially in the human retina
… I, were selected for expression analysis by RT-PCR in a great number of human tissues. This resulted in the identification of 16 genes which were expressed exclusively in the retina, 13 which were highly expressed in the retina compared to other tissues, 12 genes which were specifically …
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Modulation of the progesterone receptor by progestogens, antiretroviral drugs and the glucocorticoid receptor
… investigated on endogenous genes by quantitative real-time PCR (real-time qPCR) in the MDA-MB-231 PR-B-stably expressing (MDA-PR-B+) cells. Once the progestogen responses via the PR were characterised, PR-B activity in the presence of three ARVs namely; tenofovir disoproxil fumarate (TDF), …
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Ανάπτυξη και κλινικές εφαρμογές μεθόδων μοριακής διαγνωστικής για την ανίχνευση κυκλοφορούντων καρκινικών κυττάρων στον καρκίνο του μαστού μέσω της έκφρασης της μαστοσφαιρίνης α
… The method involves: a) the use of nested PCR in peripheral blood of early breast cancer patients. All primers were in silico designed and evaluated in order to avoid false positive results due to genomic DNA contamination. The specificity of the primers used was evaluated in 30 healthy …