Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 22 for “"PCR detection"”.
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Droplet digital PCR detection and next-generation sequencing of HIV-1 in breastmilk of breastfeeding women on antiretroviral treatment
… quantitation in breastmilk using droplet digital PCR (ddPCR) and sequencing of viral variants in breastmilk using Illumina MiSeq next generation sequencing to inform transmission risk and efficacy of maternal ART clinical trials. HIV-negative breastmilk samples were spiked with 8E5 cells (a cell …
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PCR Detection of Streptococcus Mutans and Streptococcus Sobrinus in Dental Plaque Samples from Low, Moderate, and High Caries Risk Children
… as detected by the polymerase chain reaction (PCR).Methods: Subjects between the ages 2 to 16 years of age were used in this study. After the subjects received a dental examination, dental plaque samples were collected by rubbing a toothpick on the buccal surface of the most posterior tooth in …
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An Alternative Method to Concentrate E. coli O157:H7 from Meat Homogenates to Facilitate Detection by Cultural and Molecular Methods
… beef with subsequent polymerase chain reaction (PCR) detection. Hypotheses that metal hydroxide concentration would lead to increased cell recoveries compared to controls and that subsequent detection by PCR would gain higher detection sensitivity were tested for E. coli O157:H7 in autoclaved and …
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MOL-PCR For SNP Based Detection and Characterization of STEC non-O157 Strains
… study, the multiplex oligonucleotide ligation-PCR (MOL-PCR) detection platform was adapted for the detection and characterization of the above-described non-O157 STEC serogroups. The SNP-based MOL-PCR assay has demonstrated the ability to simultaneously detect the non-O157 serogroups and …
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Evaluation and adaptation of molecular approaches for detection and characterization of viruses of the respiratory tract
… acids using phi29 and Bst DNA polymerases in the detection and characterization of pathogenic viruses of the respiratory tract. High Resolution Melting (HRM) analysis was used in this study for screening similar sequences to avoid unnecessary sequencing from genomic libraries and detection of …
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A Rapid and Power-Efficient Laser-Induced Graphene Micro-Thermocycler for DNA Polymerase Chain Reaction
… specific segment, polymerase chain reaction (PCR) has a wide range of applications in DNA cloning, forensics, medical, and infectious disease detection. As a result, the technology is becoming more and more widespread as the COVID-19 pandemic pushes for the need for rapid nucleic acid testing, …
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The application of molecular techniques for the rapid and sensitive detection of gastrointestinal pathogens directly in food
… rapid food testing. Molecular methods, such as PCR, offer a rapid, sensitive and specific means of detecting pathogens, however loss of sensitivity and lack of robustness have been reported when PCR is applied to heterogeneous and complex food matrices. The aim of this study was to establish a …
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Detection of enteroviruses in marine waters using RT-PCR.
… focused on (i) optimization of methods for RT-PCR detection of enteroviruses in marine waters, with particular attention to the issue of sensitivity vs inhibition (ii) comparison of enterovirus detection in marine waters by cell culture and RT-PCR and (iii) development of a method for detecting …
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The Development of New Tools to Investigate Alphavirus Replication Kinetics
… in invertebrate cells. By comparing different detection strategies and chemistries, we identified an optimal ssqPCR assay design for strand-specific quantification of viral RNAs in infected cells and tissues. We found that primer sets incorporating the use of a non-target tag sequence were able …
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Probiotics for the management of kidney stone disease
… males on a normal diet. Results obtained using PCR detection and denaturing gradient gel electrophoresis (DOGE) analyses showed differences in the oxalate-degrading bacteria between the two populations, with the black population recording a higher incidence of known oxalate-degrading bacterial …
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Micropollutants biotransformation by two nitrifying communities enriched from biofilm of a nitrification trickling tower
… reactor were 0% and 25.6% based on quantitative PCR detection. We then investigated micropollutants biotransformation by this two nitrifying enrichment culture. We used fourteen micropollutants whose biotransformation were previously observed to be inhibited by inhibitor (i.e., allythiourea and …
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The osmotic stress response of ale and lager yeast
… strain, growth-phase and solute type dependent. PCR detection revealed that the elucidated genes involved in glycerol biosynthesis were present in all production brewing strains examined. Sequence analysis of the GPDJ gene (encoding a cytoplasmic glycerol-3-phosphate dehydrogenase) revealed point …
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Characterisation and detection of viruses (Cucumovirus, Potyvirus) infecting vanilla in Réunion Island and Polynesian Islands
… CMV inoculum. Existing serological and molecular detection rests were performant for the detection of CMV directly from vanilla tissue. Secondly, this study provided the first coat protein sequence information for Vanilla mosaic virus (VanMV, Potyvirus). A Cook Islands isolate (VanMV-CI) and a …
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A generic pathogen capture technology for sepsis diagnosis
… for technologies that can accelerate pathogen detection and sepsis diagnosis. Our lab previously developed a micromagnetic-microfluidic pathogen isolation technology that can selectively remove pathogens from flowing whole human blood with high efficiency using micro- or nano-sized magnetic …
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Limits of detection of Magnaporthe oryzae Triticum pathotype in wheat seed: implications for pathogen dissemination
… objective of this research was to establish the detection threshold for MoT in infected wheat seeds as a function of sensitivity and specificity of a modified quantitative and conventional PCR assay. Accurate amount mix ratios of MoT colonized ground seeds / non-MoT colonized ground seeds were …
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Use of molecular and bioinformatics tools for developing methods of epidemiological identification of trypanosomes
… epidemiological tools are available for detection and strain typing in trypanosomes. These tools include nested ITS-PCR, which is based on size variation of the ITS genes and MGE-PCR, which is based on variations in position of mobile genetic elements (MGEs). Although commonly used, these …
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Investigation into Toxoplasma gondii infection in relation to expression of neurodegenerative disease markers in natural mice populations
… broad aims. Firstly, to investigating whether detection of T. gondii in cadaveric brain tissue of patients, who had been previously diagnosed with Alzheimer’s Disease (AD), could be used to investigate the role of this parasite in AD. Secondly to investigate T. gondii infection in naturally …
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Isolation And Molecular Characterization Of Shiga Toxin Producing Escherichia coli In Cattle, Water And Diarrhoeal Children From The Pastoral Systems Of Southwestern Uganda
… 2 were investigated for the presence of STEC by PCR detection of stx genes. During phase 1, cattle reared on range which were associated with households of sick children were investigated in parallel to the children for STEC excretion. STEC was isolated from E. coli in 7 of 80 (8.8%) children and …
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Diagnostics and epidemiology of <i>Phytophthora infestans</i> : the cause of late blight of potato
A specific and sensitive PCR assay for the detection of <i>Phytophthora infestans</i>, the cause of late blight of potato, in soil and plant tissues was developed. A <i>P. infestans</i> specific primer pair (INF FW2 and INF REV) was designed by comparing the aligned sequences of internal …
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The Microbial Quality and Safety of Produce
… industry, the final study seeks to improve rapid detection methods of foodborne pathogens associated with raw sprouts. For the first study, produce samples were collected from the southern United States throughout production and processing and assayed by enumerative tests for total aerobic …
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