Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 26 for “"PCR array"”.
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Vip And Host Immunity
… infected corneas with or without VIP treatment. PCR array and real-time RT-PCR data demonstrated that VIP treatment decreased pro-inflammatory, but increased anti-inflammatory TLRs. Immunohistochemistry, ELISA and western blot results further confirmed the PCR data. AC7 siRNA experiments …
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The Effect of Compound L19 on Human Colorectal Cells (DLD-1)
… real-time polymerase chain reaction (qRT-PCR) and gene specific primers for caspases 6, 8, 10 (extrinsic pathways), 9 (intrinsic), 1, 4, 5 and 12 (ER stress). RT<sup>2</sup> Profiler PCR array for human apoptosis genes was used to explore additional changes in apoptotic gene expression. …
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Identification of microRNA associated with systemic lupus erythematosus in Korean
… patients and 8 normal controls. The miRNA microarray chip analysis identified miRNAs differentially expressed in SLE. For the microRNA PCR arrays, we isolated total RNA from plasma according to the manufacturer's instructions. The RNAs(n=10) were pooled in each sample group with an equal amount …
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Regulation of Dkk1, an Inhibitor of the Wnt Signaling Pathway, by DAX-1 in Mouse Embryonic Stem Cells
… identified target genes of DAX-1 in mESC through PCR array methodology (Torres 2013). The Dickkopf 1 gene (Dkk1) was identified as a potential target of DAX-1 by these studies and has since been confirmed to be a DAX-1 target in mESC. However, to date very little is understood of the mechanism of …
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A histopathological and genomics study of the mutated human FAM111B gene related POIKTMP disease
… the disease in South African Following that, qRT-PCR was employed to assess gene expression changes between the patient and the familial control. The RT2 Profiler Human fibrosis PCR Array was then used to associate POIKTMP and 84 known fibrotic markers to propose a possible fibrotic pathway …
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A histopathological and genomics study of the mutated human FAM111B gene related POIKTMP disease
… the disease in South African Following that, qRT-PCR was employed to assess gene expression changes between the patient and the familial control. The RT2 Profiler Human fibrosis PCR Array was then used to associate POIKTMP and 84 known fibrotic markers to propose a possible fibrotic pathway …
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The Role of Dax-1 in Regulating Pluripotency in mouse Embryonic Stem Cells
… in mouse ES cells using RNAi followed by PCR array methodology to identify genes that were alternately regulated upon targeted knock-down of Dax-1. Several novel Dax-1 targets have been identified, including genes that are involved in the Wnt signaling pathway. In attempts to understand …
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Identifying genetic biomarkers for diagnosis of prostate cancer in South African men
… with the Human Prostate Cancer RT2 Profiler PCR Array and SYBR Green Master Mix. Data were analyzed with the GeneGlobe RT2 and miScript PCR Array Data Analysis Centre from Qiagen. Results: The cohort included patients from different ethnic groups namely, Caucasians, Mixedand African ancestry. …
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The roles of human cytomegalovirus US27 gene product during virus infection
… genes for putative G protein-coupled receptors (GPCRs): US27, US28, UL33, and UL78. The US28 gene product is a functional chemokine receptor that exhibits a variety of signaling outcomes. In contrast, the US27 gene product is an orphan with no known chemokine ligands. We have found that US27 …
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Investigation of microRNA expression in thyroid carcinoma among South Africa patients
… study. MiRNA miScript polymerase chain reaction (PCR) Array (Qiagen) was used to determine the differential miRNA expression profiles between follicular thyroid carcinoma (FTC) and papillary thyroid carcinoma (PTC). Real time PCR was employed to confirm the expression levels of miRNA- 21 and …
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HIV alters the expression of miRNA hsa-miR-200c-3p in B-cells, leading to enhanced migration of lymphoma cells
… were identified as potentially important in a PCR array, and characterise their roles in Burkitt’s lymphoma cells exposed to an attenuated strain of HIV-1, compared to control cells. Methods: Single-tube TaqMan miRNA assays were used to validate the previously observed differential expression …
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Extracellular Matrix-Derived Microcarriers as 3-D Cell Culture Platforms
… flasks under proliferation conditions, with PCR array and global protein analyses suggesting that the DCT microcarriers may have predisposed the cells towards the chondrogenic lineage. Building on previous work, the final goal was to investigate the adipogenic differentiation of hASCs …
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The effects of the adipocyte-secreted proteins resistin and visfatin on the pancreatic beta-cell
… adipokines was also measured in the beta-cell. PCR array analysis showed that resistin and visfatin treatment resulted in significant changes in the expression of key beta-cell specific genes. Interestingly, both resistin and visfatin are highly expressed in the beta-cells. This suggests that …
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Modeling host responses to tick feeding and tick-borne flavivirus evolution and dispersal
… andersoni nymphs was characterized using PCR-array, microarray, histopathology, and protein-detection methods. These studies show a pro-inflammatory innate-like immune response characterized by cytokines IL-1b, IL-6, IL-10, chemotaxis of neutrophils and monocytes, wound healing responses, …
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Chronic Stress Promotes Tumor Growth Through Increased Bdnf Production and Neo-Innervation
… tumors are recruiting nerve growth, I utilized a PCR array analysis of 84 nerve growth related genes and their receptors, which showed that stimulation of the SKOV3 ovarian cancer cell line with norepinephrine (NE) leads to increased expression of several neurotrophins, including brain-derived …
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The relationship between glucose metabolism byproduct, D-lactate, and vascular endothelial cell dysfunction and possible role in diabetes
… eNOS, PI3K) were measured using Quantitative RT-PCR. DLA downregulated all genes at 6 and 24 hours, followed by increase in expression after 48 hours except PI3K, which remained below control. To further investigate apoptosis, the Human Apoptosis PCR Array was used and expression of all …
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Development and Utilization of Novel Immunologic Reagents for Infectious Disease Studies in the Guinea Pig
Genital herpes disease caused by HSV-2 infections is a worldwide public health epidemic with a global prevalence estimated at 500 million. These individuals serve as a reservoir for transmission of the virus to naïve individuals resulting in a global increase of 26 million new infections per year. …
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Estrogen Modulation of Cardiac Immune Cells Is Cardioprotective.
… left ventricle were examined using a real time PCR array. A total of 55 inflammatory genes which were modified (> 2-fold change) at three days post-fistula was reduced to 20 genes by estrogen treatment, whereas 12 genes were comparably modulated in both fistula groups. In decreasing order, the …
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DIFFERENTIAL EFFECTS OF GROWTH FACTORS ON GLYCOLYSIS IN OVARIAN CANCER CELLS
… metabolism. We used the glucose metabolism RT-PCR array to profile expression of glycolytic genes. The most remarkable change induced by LPA and EGF was the robust induction of hexokinase 2 (HK2) that stimulates irreversible entry of glucose to the glycolytic pathway. However, insulin and IGF-1 …
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Modulation of the progesterone receptor by progestogens, antiretroviral drugs and the glucocorticoid receptor
… on endogenous genes by quantitative real-time PCR (real-time qPCR) in the MDA-MB-231 PR-B-stably expressing (MDA-PR-B+) cells. Once the progestogen responses via the PR were characterised, PR-B activity in the presence of three ARVs namely; tenofovir disoproxil fumarate (TDF), dapivirine (DPV) …
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