Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 44 for “"PCR Primers"”.
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MUPrimer : a tool for finding allele specific PCR-primers for homologous gene sequences
… AT AUTHOR'S REQUEST.] Polymerase chain reaction (PCR) amplification of specific regions of DNA has proven to be a powerful tool in molecular biology research. Synthetic short oligonucleotide DNA sequences (primers) provide specificity for the reaction by annealing to the target template DNA and …
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Phylogenetic Analysis of the Mycobacteria and Diagnosis of Paratuberculosis by 16SrRNA Amplification and Probing
… probes and polymerase chain reaction (PCR) primers for the detection and identification of mycobacteria. To increase sensitivity of detection of mycobacteria in natural samples, five sets of rRNA-targeted PCR primers were designed to amplify various probe target sites. Like the probes, …
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Bioluminescence in dinoflagellates – diversity, molecular phylogeny and field ecology
… “universal” and taxon specific PCR primers designed to amplify the luciferase gene (lcf), the distribution of this gene within dinoflagellates was found to be taxon specific and the first lcf sequences obtained from four genetically distant genera revealed a previously unknown …
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Degenerate oligonucleotide primed amplification of genomic DNA for combinatorial screening libraries and strain enrichment
… using degenerate oligonucleotide primed PCR (DOP-PCR). The approach can produce DNA libraries from nanograms of a template genome or the metagenome of multiple microbial populations. The DOP-PCR primers contain random bases, and thermodynamics of hairpin formation was used to design …
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Expression and regulation of genes associated with CNS regeneration and plasticity
… Chromatin immunoprecipitation assay 18 9. ChIP-PCR primers 19 10. qRT-PCR primers 20 11. Methylation assay 21 12. Tissue processing and immunohistochemistry 21 13. Quantification 22 14. Production of recombinant adeno-associated virus (AAV) 23 15. Statistical Analysis 23 III. RESULTS 25 Part A. …
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Expression of single chain antibody-encoding genes in fission yeast and tobacco
… cells. During the course of this research, a new PCR-based strategy was developed that significantly reduced the time required for SCA gene synthesis. Although initially developed for the synthesis of SCA genes from templates of known sequence, PCR primers have now been developed that have …
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High Diversity of Trypanosoma Cruzi Discrete Typing Units Circulating in Triatoma in Western Mexico
… cruzi </em>infection was detected using PCR primers specific for the minicircle variable region of the parasite’s kinetoplast DNA (kDNA). <em>T</em><em>rypanosoma cruzi</em> DTUs were identified by amplifying the intergenic region of the mini-exon, and the genes <em>24S</em><em>α, …
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Molecular analysis of algal communities in the San Joaquin River
… river water samples. Polymerase Chain Reaction (PCR) primers were designed to amplify the 18S rRNA gene of certain taxonomic groups of freshwater algae; there was limited success in specific amplification. Additionally, a primer pair utilizing both the 16S plastid gene and the 16S rRNA gene was …
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Identification and isolation of microsatellite loci from the Trematode Echinostoma Trivolvis for use in interspecific and intraspecific variation studies
… variable nature and reported ease of use with PCR. Parasite DNA was extracted from planorbid snails from several locations within California including: Point Reyes National Seashore, Lake Tahoe, and the San Francisco Bay Area. In addition, parasite samples were obtained from Manitoba, Canada. …
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Cartilage mechanobiology : the effects of loading on the fine structure and function of chondroitin sulfate glycosaminoglycans
… of the bovine genome had to be sequenced, PCR primers designed, and bulk expression levels determined. Static compression resulted in the significant up-regulation of two genes of interest: chondroitin sulfate and GalNAc 4S,6-sulfotransferase.
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Distribution of the Cytotoxin Gene in Pseudomonas Aeruginosa
… is undetectable with oligonucleotide probes or PCR primers derived from the cytotoxin gene of PA158 . oligonucleotides. The sequences of these probes were derived from the _cytotoxin gene of Ps. aerug.inosa strain PA158. They were designed to hybridize withs· and 3' regions of the cytotoxin …
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Detecting a Probiotic Product Within the Gut of Broiler Chickens
… and ceca. Species-specific and strain specific PCR primers were employed for probiotic detection. Wild strains of <em>P. acidilactici</em>, <em>P. pentosaceus</em>, and <em>L. plantarum</em> were detected in the feeds, inhibiting detection of the probiotic strains when using species-specific PCR …
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Prevalence and Identity of Tissue Cyst Forming Apicomplexan Parasites in the Muscles of Raptors
… via histology, Polymerase Chain Reaction (PCR) and Restriction Fragment Length Polymorphism (RFLP) using DraI and HinfI enzymes (Sarocystis only). Of 187 available HandE stained tissue sections, 33 contained sarcocysts. Nineteen of these slides had a matching DNA sample to compare via PCR. …
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Discovery and application of novel antimicrobials against the protozoan Histomonas meleagridis: the causative agent of Histomonosis in poultry
… study tested a range of published H. meleagridis PCR primers. It was found that there was found that the Primers tested either did not amplify an amplicon at the correct size or produced amplicons for samples which did not contain H. meleagridis. To try and help with future detection there was an …
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Development of a single real-time RT-PCR method for the group-specific identification of African horsesickness virus and bluetongue virus
… present study, a duplex one-step real-time RT-PCR using the fluorogenic dye SYBR® Green I was developed for the specific detection and identification of AHSV and BTV in one reaction, using melting temperatures (Tm) to discriminate between the viruses. Two primers pairs were designed to bind to …
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METHYLATED AMINE-UTILISING BACTERIA AND MICROBIAL NITROGEN CYCLING IN MOVILE CAVE
… (MMA) metabolism were investigated using new PCR primers designed to target gmaS, the gene for gammaglutamylmethylamide synthetase, a key enzyme of the recently characterised indirect MMA oxidation pathway. This pathway is also present in bacteria that use MMA only as a nitrogen source, while …
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Marine algal virus communities along Southern African coasts
… samples using algal - virus specific PCR primers AVS1 and POL. Amplified fragments were then sequenced. Viral sequences were identified and mapped to existing virus families. Amplicon specific primers were designed for select dominant virus group identified for both bloom events. These …
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Assessment of flagellate diversity at deep-sea hydrothermal vents using the combined approach of culture-dependent and culture-independent methods
… gradient gel electrophoresis (DGGE) using PCR primers specific to the kinetoplastid clade. By comparing these two different methods of analysis, my goal was to decrease the biases and/or errors inherent in either method alone and to improve our ability to assess flagellate diversity and …
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Functional analysis of soybean PR-10 genes
… from in-house microarray studies. Gene-specific PCR primers were designed to clone full-length cDNA of selected PR-10 genes. The cDNA were sequence verified and transferred into an Agrobacterium overexpression vector, expression controlled by the CaMV35S constitutive promoter. Four PR-10 family …
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Detection and further characterisation of the toxins and associated genes of Bacillus cereus
… methods, including polymerase chain reaction (PCR), gel diffusion haemolysis, Vero cell cytotoxicity and two commercially available diarrhoeal toxin detection kits. The genes for all five of the diarrhoeal toxins (haemolysin BL, enterotoxin T, non-haemolytic enterotoxin, enterotoxin FM and …
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