Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 13 of 13 for “"PCR Cloning"”.
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Use of PCR Cloning Combined with DNA Barcoding to Identify Fish in a Mixed-Species Product
… are present within the same food product. PCR cloning has high potential to be used in combination with DNA barcoding to overcome this challenge. The objective of this study was to examine the use of PCR cloning combined with DNA barcoding to identify fish in a mixed-species product that …
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Isolation, identification and characterisation of three viruses from Pisum sativum in South Africa
… studies and molecular-based techniques such as PCR, cloning and sequencing and sequence comparisons.
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Evolutionary Dynamics of the Alpha-actinin Gene Family
… studies of the a-actinin gene family, I used RT-PCR, cloning and automated DNA sequencing to obtain 77bp of sequence for what may be yet another member of the spectrin superfamily, the G3.5 antigen.
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Polymorphism of bovine MHC class II genes: Association with persistent lymphocytosis caused by bovine leukemia virus
… was confirmed by a polymerase chain reaction (PCR)-based typing method in twenty-six unrelated cows, 13 of which were PL. A 783 bp BoLA-DQA cDNA clone $(\alpha$5) was identified by screening a bovine cDNA library. The $\alpha$5 clone has an average nucleotide similarity of 91.6%, 86.0% and …
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In situ formed magnetic ionic liquids: DNA extraction performance and fluorescence-compatibility in bioanalytical applications
… procedures such as polymerase chain reaction (PCR), cloning, and gene therapy. Obtaining high yields and pure DNA presents a significant sample preparation challenge in nucleic acid analysis. Current methodologies such as the phenol-chloroform extraction use toxic organic solvents and …
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Investigation of the Substrate Recognition Characteristics and Kinetics of Mammalian Mitochondrial DNA Topoisomerase I
… experiments of currently available methods for PCR-cloning. These mtDNA containing recombinant plasmids were used in a kinetic analysis of the highly purified enzyme. Recombinant plasmids containing the bovine mtDNA heavy and light strand origins of replication (pZT-Hori and pZT-Lori, …
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Molecular Ecology and Evolution of Fibrobacter and Its Cellulases
… within species F. succinogenes were recovered by PCR cloning from the cecum of a pony. In searching for differentiating characteristics, fiber digesting properties of Fibrobacter were further investigated. A cellulase gene, cel-3, that codes for endoglucanase 3 was chosen for comparative studies. …
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Functional analyses of ζ-crystallin binding to the 3'-UTR of glutaminase (GA) mRNA
… coding and 3'-untranslated sequence. PCR cloning and sequencing confirmed that the 4.5 kb GA mRNA is the homolog of the human GA mRNA. Sequence analysis established that the 3'-untranslated regions (3'-UTR) of the 4.5 kb GA mRNA contains the 8-base adenylate-uridylate (AU) sequence …
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The characterization of MHC Class II genes of the Nile crocodile (Crocodylus niloticus) : an investigation of mechanisms that shape genetic diversity in natural populations
… the Nile crocodile, Crocodylus niloticus. Using PCR-cloning-sequencing methodology, allelic diversity at MHC Class II genes was investigated and provides evidence for at least two Class II 13 gene families in the Nile crocodile. The Crni-OAB family is homologous to classical Class II vertebrate …
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Site-Directed Mutagenesis in Francisella Tularensis by Allelic
… were constructed based on the commercial PCR cloning vector pSC-A. These vectors were created by inserting into the cloning site a kanamycin resistance gene boarded upstream by 1.3 kb of N-terminal DNA and downstream by 1.3 kb of C-terminal DNA that flanks the target gene. …
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Molecular characterization of mycoplasmas species isolated from the genital tract of Dorper sheep in South Africa
… methods. In this study, 16SrRNA gene-based PCR assays and gene sequencing was used for the detection and characterization of Mycoplasma species from cases of ulcerative vulvovaginitis and balanoposthitis in Dorper sheep in South Africa. This investigation was conducted on 34 stored field …
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Aetiological links between oral pathogens and dementia.
… of AD pathology. Methods: Molecular techniques (PCR, cloning and sequencing) were employed for investigating the presence of bacterial DNA within the specimens (human or mouse) using two different approaches (universal and species specific primers). The presence of specific virulence factors were …
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The Isolation and Characterization of the Microbial Flora in the Alimentary Canal of <em>Gromphadorhina portentosa</em> Based on rDNA Sequences.
… and Eukaryotic 16s/18s rRNA were then used for PCR amplification of total DNA isolated from gut contents. Sequences from isolates were compared using BLAST, ClustalW, and other programs to recognize the isolates' identities and place them using a phylogenetic tree analysis. Eubacterial, …