Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 12 of 12 for “"PCR (Polymerase Chain Reaction)"”.
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Oligonucleotide Design for Whole Genome Tiling Arrays
… They have many important applications including PCR (polymerase chain reaction) amplification, microarrays, or FISH (fluorescence in situ hybridization) probes. While traditional microarrays are commonly used for measuring gene expression levels by probing for sequences of known and predicted …
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Using Capillary Electrophoresis for High-Throughput Molecular Screening
… and the subsequent amplification of aptamers by PCR (polymerase chain reaction). Several screening methodologies have been proposed, including systematic evolution of ligands by exponential enrichment (SELEX) and nonequilibrium capillary electrophoresis of equilibrium mixtures (NECEEM). However, …
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Fatores de virulência de Bacillus cereus isolado na cadeia produtiva do leite na microrregião de Viçosa, Minas Gerais
… Os 32 isolados foram submetidos a análises de PCR (Polymerase Chain Reaction) para a detecção do gene gyrB. Em todos os isolados foi amplificado um produto de 352pb que após digestão utilizando e enzima de restrição RsaI apresentaram tamanhos de aproximadamente 250pb, resultados que de acordo …
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Variabilidade genética no Complexo Principal de Histocompatibilidade (MHC) de três espécies de mamíferos marinhos da costa do Rio grande do Sul
… do exon 2 do gene DQB do MHC foi amplificada por PCR (Polymerase Chain Reaction) em 109 amostras de toninhas (Rio de Janeiro n=32, Rio Grande do Sul n=52, Argentina n=25), 35 amostras de lobo marinho sul-americano e 30 amostras de baleia franca austral, utilizando-se um par de primers heterólogos. …
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The identification and recording of potential biological hazards in ancient monuments for inclusion in HBIM within refurbishment and demolition in ancient castles
… of specimens from site were analysed using a PCR, (Polymerase Chain Reaction), to extract DNA as to determine the Genus within the community. All results have been portrayed within a HBIM model and data management system to ascertain the required Health and Safety legislation is required for …
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Effekte von Leptin- und MCP-1-Applikation auf funktionelle Parameter der Arteriogenese : eine experimentelle Studie mittels Ultraschallflussmessungen, Mikrosphärperfusion und Proliferationsanalyse
… Leptin (22,67±1,17%; p=ns). Durch Real-Time PCR (polymerase chain reaction) gemessene Ki-67 mRNA zeigte einen 8,8±6,1-fachen <br>(p<0,01) Anstieg in proliferierenden Kollateralarterien, der durch MCP-1-Behandlung weiter 3,9±3,1-fach (p<0,05) anstieg. <br>MCP-1 und Leptin erhöhten kollateralen …
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Dna Aptamers Selected Against Wild-Type Helix 69 Ribosomal Rna And Their Implications In Combating Antibiotic Resistance
… recovered, and enriched by direct-bead PCR (polymerase chain reaction). Levels of bound DNA and diversity of the amplified library pools were monitored by UV-visible spectroscopy and sequencing between rounds of SELEX (11 total). Select rounds were cloned and sequenced. Consensus …
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The molecular biology of calbindin-D₉
… calbindin-D9k were cloned using a modified PCR (polymerase chain reaction) technique called RACE (rapid amplification of cDNAs ends) with rat and bovine cDNA sequence-derived primers for amplification. The full length of these sequences was confirmed by primer extension assay. The human cDNA …
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Detection of the calreticulin type 1 and type 2 mutations in a group of myeloproliferative neoplasm patients using molecular methods
… study aimed to evaluate the use of conventional PCR followed by restriction enzyme digestion, confirmation of the PCR results by nucleotide sequencing, as well as real-time PCR (qPCR) to detect these mutations. A conventional PCR commercial kit was also compared to that of the developed …
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Quantifying Potential Sources of Microbial Contamination in Household Drinking Water Samples
… and chemical source tracking techniques (Polymerase Chain Reaction (PCR) and fluorometry, respectively) identified 4 of 26 samples as likely contaminated with human wastewater. Application of these source-tracking analyses on a larger scale will prove useful in defining remediation …
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Cell-free fetal DNA (cffDNA) enrichment for non-invasive prenatal testing (NIPT): a comparison of molecular techniques
… at lower denaturation temperature polymerase chain reaction (COLD-PCR), inverse-PCR and Pippin Prep™ gel electrophoresis, were analysed to identify a novel approach to selectively enrich shorter cffDNA fragments from larger maternal cell-free DNA (cfDNA). The sensitivity of droplet …
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PCR-Slide: a modular and cascadable platform for DNA sample processing with integrated nanoliter dosage
… for performance of DNA-related multistep reactions based on ther-mal cycling. Compared to the state of the art, the expenditure of laboratory equipment for DNA-based assays was reduced significantly. <br>The platform that was developed within the scope of this thesis consists of three …