Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 77 for “"PCNA"”.
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The role of UBZ1 in PCNA-dependent DNA damage response
… 11 and K, Rad18 and WHIP. Moreover, Ubzl has a PCNA Interacting Peptide (PIP), which is important for its interaction with the Proliferating Cell Nuclear Antigen (PCNA). Besides these two domains, a third domain known as the SprT domain, which is of unknown function, is also been found in Ubzl. …
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RAD6-abhängige DNA-Reparatur wird durch Ubiquitin- und SUMO-Modifikation von PCNA reguliert
… sind bisher allerdings wenig verstanden. PCNA (Proliferating Cell Nuclear Antigen)ist eine zentrale Komponente der DNA-Replikation und DNA-Reparatur.Im Rahmen dieser Arbeit konnte gezeigt werden,daß PCNA über drei verschiedene posttranslationale Modifikationen reguliert wird.In der S-Phase …
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Der diagnostische Stellenwert der MIB-1- und PCNA-Immunhistometrie in der Differentialdiagnostik follikulärer Schilddrüsentumoren
The diagnostic value of MIB-1 and PCNA immunohistometry in differential diagnosis of follicular tumors of the thyroid gland The cellular prolifarative activity MIB-1 and PCNA immunopositivity of normal tissue (n = 30), follicular adenoma (n = 21) and follicular carcinoma (n = 28) of the thyroid …
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Avaliação da expressão do PCNA em epitélio lingual de camundongos submetidos à ingestão e aplicação tópica de alcool à 40°GL
… as células positivas para a evidenciação do PCNA (Antígeno Nuclear de Proliferação Celular), pela técnica imunohistoquímica. Foram consideradas as células das camadas basal e intermediária do epitélio lingual. A contagem foi realizada em três momentos do trabalho; antes dos animais serem …
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Single-molecule and ensemble studies of DNA replication system
… from ensemble experiments, studying the clamp (PCNA) and the clamp loader (RFC) from the mesophilic archaeon Methanosarcina acetivorans, and then moved to the single-molecule level trying to reconstitute the DNA replication machinery protein by protein. In the first study, by developing a …
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Discovery of Molecules that Modulate Protein-Protein Interactions in the Context of Human Proliferating Cell Nuclear Antigen-Associated Processes of DNA Replication and Damage Repair
… complex Proliferating Cell Nuclear Antigen (PCNA) that encircles chromatin-bound DNA and functionally acts as a DNA clamp that provides topological sites for recruitment of proteins necessary for DNA replication and damage repair. PCNA has critical roles in the survival and proliferation of …
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Comparative Studies of Processivity Clamp Loader ATP Site Function
… clamp, proliferating cell nuclear antigen (PCNA), onto DNA. ATP binding to RFC activates a γ-phosphate sensor in ATP site C that promotes DNA association by RFC-PCNA. DNA binding to the RFC-PCNA complex then triggers the ATP hydrolysis cycle which starts in site C and ends with ATP site D. …
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Clamp loading on the leading strand during DNA replication and initial reconstitution of sister chromatid cohesion establishment
… factors. One such factor is the sliding clamp PCNA, which acts as both a processivity factor to support fast and efficient DNA synthesis and as a recruitment platform in various genomic stability maintenance processes downstream of DNA replication. Regulation of chromatin bound PCNA is …
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Biochemical and Genetic Studies on Origin Recognition and DNA Synthesis From the Mesophilic Archaeon Methanosarcina Acetivorans
… these activities were stimulated by the cognate PCNA. Fascinatingly, unlike most translesion DNA polymerases previously described, MacDinB synthesized an unusually long product (∼7.2 kb) in the presence of the sliding clamp. A PCNA-interacting peptide (PIP) box located at the C-terminus of …
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Insights into the regulation of the CRL4D̳T̳L̳ ubiquitin ligase
… to S phase and after DNA damage through a PCNA-dependent mechanism. Recent studies have focused on elucidating the requirements within the substrates that dictate CRL4DTL-mediated degradation. The majority of identified substrates have a specialized PCNA interaction peptide motif (PIP box) …
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The roles of DNA(cytosine-5) methyltransferase1 in carcinogenesis related to cellular factors, virus and chemicals
… hence suppressing the early genes. DNMT1 and PCNA was found to interact with two distinct regions of Hsp70. The binding site of Hsp70 was localized to the N-terminal of DNMT1. The disruption of this site in DNMT1b abolished its interaction with Hsp70. Activation of the ATPase activity of Hsp70 …
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Replication-associated base excision repair Of oxidized bases in the mammalian genome
… A (RPA), Proliferating Cell Nuclear Antigen (PCNA), Flap Endonuclease 1 (FEN1), DNA Polymerase ä, Replication Factor C (RFC), and DNA Ligase I as well as the stress responsive Rad9-Rad1-Hus1 (9-1-1) DNA sliding clamp. We mapped the overlapping binding sites for all of these interacting protein …
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Development of a TIRF Microscope for Single Molecule Binding Studies
… to study the crucial interaction between PCNA and CAF-1 in replication-coupled nucleosome assembly to contribute to epigenetic inheritance. The microscope measures fluorescence following total internal reflection (TIR) to accomplish single molecule imaging (SMI). TIR provides a high …
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Avaliação imuno-histoquímica da proliferação e morte celular em neoplasias mamárias malignas caninas
… was applied by means of the LSAB method; anti-PCNA, anti-Ki-67 were used as primary antibodies, as well as cleaved anti-Caspase-3. Considering 61 samples, by means of the histopathology procedure, five carcinosarcomas, nine carcinomas in mixed tumors, six complex tubular adenocarcinomas, 12 …
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Analysis of Interphase Chromatin Motion in HeLa Cells
… and proliferating cell nuclear antigen (PCNA) were followed through interphase. Chromatin motion was modelled as a two dimensional random walk. Reduction in chromatin mobility was observed during S phase was dependent on proximity to the replication machinery. Mobility during G1 and G2 …
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