Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 70 for “"PCNA"”.
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The role of UBZ1 in PCNA-dependent DNA damage response
… 11 and K, Rad18 and WHIP. Moreover, Ubzl has a PCNA Interacting Peptide (PIP), which is important for its interaction with the Proliferating Cell Nuclear Antigen (PCNA). Besides these two domains, a third domain known as the SprT domain, which is of unknown function, is also been found in Ubzl. …
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RAD6-abhängige DNA-Reparatur wird durch Ubiquitin- und SUMO-Modifikation von PCNA reguliert
… sind bisher allerdings wenig verstanden. PCNA (Proliferating Cell Nuclear Antigen)ist eine zentrale Komponente der DNA-Replikation und DNA-Reparatur.Im Rahmen dieser Arbeit konnte gezeigt werden,daß PCNA über drei verschiedene posttranslationale Modifikationen reguliert wird.In der S-Phase …
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Avaliação da expressão do PCNA em epitélio lingual de camundongos submetidos à ingestão e aplicação tópica de alcool à 40°GL
… as células positivas para a evidenciação do PCNA (Antígeno Nuclear de Proliferação Celular), pela técnica imunohistoquímica. Foram consideradas as células das camadas basal e intermediária do epitélio lingual. A contagem foi realizada em três momentos do trabalho; antes dos animais serem …
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Single-molecule and ensemble studies of DNA replication system
… from ensemble experiments, studying the clamp (PCNA) and the clamp loader (RFC) from the mesophilic archaeon Methanosarcina acetivorans, and then moved to the single-molecule level trying to reconstitute the DNA replication machinery protein by protein. In the first study, by developing a …
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Discovery of Molecules that Modulate Protein-Protein Interactions in the Context of Human Proliferating Cell Nuclear Antigen-Associated Processes of DNA Replication and Damage Repair
… complex Proliferating Cell Nuclear Antigen (PCNA) that encircles chromatin-bound DNA and functionally acts as a DNA clamp that provides topological sites for recruitment of proteins necessary for DNA replication and damage repair. PCNA has critical roles in the survival and proliferation of …
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Comparative Studies of Processivity Clamp Loader ATP Site Function
… clamp, proliferating cell nuclear antigen (PCNA), onto DNA. ATP binding to RFC activates a γ-phosphate sensor in ATP site C that promotes DNA association by RFC-PCNA. DNA binding to the RFC-PCNA complex then triggers the ATP hydrolysis cycle which starts in site C and ends with ATP site D. …
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Clamp loading on the leading strand during DNA replication and initial reconstitution of sister chromatid cohesion establishment
… factors. One such factor is the sliding clamp PCNA, which acts as both a processivity factor to support fast and efficient DNA synthesis and as a recruitment platform in various genomic stability maintenance processes downstream of DNA replication. Regulation of chromatin bound PCNA is …
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Biochemical and Genetic Studies on Origin Recognition and DNA Synthesis From the Mesophilic Archaeon Methanosarcina Acetivorans
… these activities were stimulated by the cognate PCNA. Fascinatingly, unlike most translesion DNA polymerases previously described, MacDinB synthesized an unusually long product (∼7.2 kb) in the presence of the sliding clamp. A PCNA-interacting peptide (PIP) box located at the C-terminus of …
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Insights into the regulation of the CRL4D̳T̳L̳ ubiquitin ligase
… to S phase and after DNA damage through a PCNA-dependent mechanism. Recent studies have focused on elucidating the requirements within the substrates that dictate CRL4DTL-mediated degradation. The majority of identified substrates have a specialized PCNA interaction peptide motif (PIP box) …
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The roles of DNA(cytosine-5) methyltransferase1 in carcinogenesis related to cellular factors, virus and chemicals
… hence suppressing the early genes. DNMT1 and PCNA was found to interact with two distinct regions of Hsp70. The binding site of Hsp70 was localized to the N-terminal of DNMT1. The disruption of this site in DNMT1b abolished its interaction with Hsp70. Activation of the ATPase activity of Hsp70 …
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Replication-associated base excision repair Of oxidized bases in the mammalian genome
… A (RPA), Proliferating Cell Nuclear Antigen (PCNA), Flap Endonuclease 1 (FEN1), DNA Polymerase ä, Replication Factor C (RFC), and DNA Ligase I as well as the stress responsive Rad9-Rad1-Hus1 (9-1-1) DNA sliding clamp. We mapped the overlapping binding sites for all of these interacting protein …
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Development of a TIRF Microscope for Single Molecule Binding Studies
… to study the crucial interaction between PCNA and CAF-1 in replication-coupled nucleosome assembly to contribute to epigenetic inheritance. The microscope measures fluorescence following total internal reflection (TIR) to accomplish single molecule imaging (SMI). TIR provides a high …
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Avaliação imuno-histoquímica da proliferação e morte celular em neoplasias mamárias malignas caninas
… was applied by means of the LSAB method; anti-PCNA, anti-Ki-67 were used as primary antibodies, as well as cleaved anti-Caspase-3. Considering 61 samples, by means of the histopathology procedure, five carcinosarcomas, nine carcinomas in mixed tumors, six complex tubular adenocarcinomas, 12 …
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Analysis of Interphase Chromatin Motion in HeLa Cells
… and proliferating cell nuclear antigen (PCNA) were followed through interphase. Chromatin motion was modelled as a two dimensional random walk. Reduction in chromatin mobility was observed during S phase was dependent on proximity to the replication machinery. Mobility during G1 and G2 …
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Analysis of cyclin-CDK mediated regulation of the G1/S transition in post quiescent murine fibroblasts
… CDK inhibition reduced levels of CDC6, MCM2 and PCNA in all contexts. Evaluation of the role of individual cyclin-CDK complexes was performed by siRNA mediated depletion. Cyclin E1/E2 depletion showed that Cyclin E regulates CDC6, MCM2 and PCNA at the transcriptional level; whereas Cyclin A …
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Regulación de la rutas de tolerancia a daños replicativos dependientes de RAD6, RAD5 y RAD52 durante el ciclo celular en Saccharomyces cerevisiae
… con la maquinaria de TLS (ubiquitinación de PCNA mediada por Rad6/Rad18 y las polimerasas Rev1/Pol z) para reparar las lesiones de ssDNA a través de una función no recombinogénica, y en consecuencia son necesarias para la mutagénesis inducida por daño en el ADN. Específicamente, se requieren …
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Making Molecular Movies: Using Single-Molecule Techniques to Unveil Hidden Features of Protein-Chromatin Interactions
… clamp, proliferating nuclear cell antigen (PCNA). PCNA is an essential ring-shaped protein required for replication in addition to diverse cellular processes including DNA repair, chromatin maintenance, and sister chromatid cohesion. To perform its functions, it must be loaded onto and …
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Phosphorylation of the Estrogen Receptor Alpha (ERα) at Serine 118 by Phospho-p44/42 MAPK and Regulation by Estrogen and Progesterone in Human Uterine Leiomyoma Tissue and Cells
… MAPK), and proliferating cell nuclear antigen (PCNA) in human leiomyoma and myometrial tissues during the proliferative and secretory phases of the menstrual cycle. <em>In vitro</em> studies to assess proliferation of uterine leiomyoma (UtLM) and uterine smooth muscle (UtSMC) cells and …
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Transcriptome Analysis of Sea Lamprey Embryogenesis
… (signal transducer), TRAF6 (signal transducer), PCNA (DNA replication), SLX4 (endonuclease), and PXN (focal adhesion) were selected for their NGS expression levels, GO annotations, and biological function significance. Their NGS expression levels were verified using Real-Time PCR (qPCR). TGFBR1 …
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