Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 44 for “"PC12 cells"”.
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Molecular regulation of neuronal apoptosis in PC12 cells.
In this study apoptosis was induced in neuronal PC12 cells by 2-5 $\mu$M VM26 (teniposide, a known apoptotic inducer) and changes in the expression of genes considered to be involved in apoptosis were examined by RT-PCR. Cell death, DNA fragmentation and morphological changes associated with …
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The crosstalk between the ERK and the cAMP signalling pathways in PC12 Cells
… survival, proliferation and differentiation. In PC12 cells, the ERK signalling pathway integrates different external stimuli: epidermal growth factor (EGF) stimulates the ERK pathway transiently and induces cell proliferation, whereas nerve growth factor (NGF) induces sustained activation of ERK …
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Type I PIP5Kα negatively regulates NGF-induced neurite outgrowth in PC12 cells
… outgrowth. For this, I generated a stable PC12 cell line lacking PIP5KIa by microRNA (miRNA) expression, leading to decreased PtdIns(4,5)P2 level. Results show that neurite outgrowth induced by nerve growth factor (NGF) was more prominent in PIP5KIa-deficient cells than in control …
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Effects of nerve growth factor on TGF-Beta,Smad signal transduction in PC12 cells
… is characterized in rat pheochromocytoma cells (PC12) which are frequently used as a model system for neuronal differentiation. PC12 cells were found to be unresponsive to TGF-ß due to limiting levels of TßRII. However, stimulation with NGF results in initiation of Smad-mediated …
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The establishment and characterization of an improved cell-free assay for exocytosis in neuroendocrine PC12 cells
… beschichteten Objektträgern wachsende PC12-Zellen wurden durch einen Ultraschallpuls in einer kleinen Kammer direkt auf dem Objekttisch eines Fluoreszenzmikroskops aufgeschlossen. Durch Ca2+ konnten eGFP-beladenen Vesikel der so hergestellten "membrane lawns" zur Exozytose und damit zur …
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Expression and Requirement of Epithelial Fatty Acid- Binding Protein in Neuronal Axon Growth
… test this hypothesis, E-FABP expression in both PC12 cells and primary retinal neurons was examined. In PC12 cells, NGF induces E-FABP mRNA and protein during the period of neurite outgrowth, and E-FABP localizes to the perinuclear cytoplasm, nucleus, and growth cone. Furthermore, …
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Pheochromocytoma-induced cardiomyopathy : a model of synergistic effects of multifactorial tumor secretions
… by catecholamine-secreting pheochromocytoma cells. Pheochromocytomas are widely believed to induce cardiomyopathy via hypersecretion of catecholamines including norepinephrine (NE). NE can have direct cardiomyocyte toxicity and can stimulate myocardial remodeling secondary to the induction of …
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MacMARCKS and the Protein Kinase C Signal Transduction Pathway: Role in Neural Secretion and Neural Development
… manner in rat brain synaptosomes and PC12 cells. It is localized to cell bodies and neurite tips of PC12 cells as well as being present on synaptic vesicles. A dominant-negative mutation in the MacMARCKS effector domain which abolishes PKC-dependent phosphorylation is associated with …
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Effects of tumor necrosis factor alpha on nitric oxide synthase in nerve growth factor-responding cells
… oxide synthase (NOS), in rat pheochromocytoma PC12 cells. NGF/TNFa-promoted iNOS can be toxic in PC12 cells. Using PC12 cell mutants lacking the low affinity p75NTR receptor, we also demonstrate that this receptor is required to mediate iNOS expression, likely acting through the transcription …
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Zinc ion homeostasis in cellular physiology and experimental models of traumatic brain injury
… and differentiated rat pheochromocytoma (PC12) cells and in rat glioma (C6) cells. When PC12 cells proliferate, [Zn2+]i undergoes precisely controlled fluctuations with two peaks within one cell cycle. These results demonstrate that the already established requirement for zinc in the cell …
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NADPH oxidase in PC12 cell differentiation and apoptosis
… the common factor leading to neuronal death. PC12 cells differentiate into sympathetic-like neurons in the presence of nerve growth factor (NGF). Once terminally differentiated, PC12 cells undergo apoptosis following NGF deprivation with similar characteristics of sympathetic neuronal death. …
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Neuroprotective Effects of FGF20 on Dopamine Neurones
… to be localised to dopamine neurones and glial cells in the rat nigrostriatal tract and in VM embryonic dopamine neurone cultures. In vitro, FGF20 protected VM embryonic dopamine neurones against 6OHDA toxicity, and, in vivo, chronic supra-nigral delivery of FGF20 protected nigrostriatal …
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Newly Synthesized Pyroglutamate Amide and its Neuroprotective Effect Against Neurotoxicity Induced Cell Death in Alzheimer's Disease
… NSP has more potent effect in inhibiting N2a and PC12 cells than the potent effect of mem due to the amide function in NSP. We also demonstrate that NSP has an inhibitory effect at a great extent on glutamate induced N2a and PC12 cells death. The inhibition of the activity of glutamate was shown …
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Disruption of 8-hydroxy-2'-deoxyguanosine DNA Glycosylase (OGG1) Antioxidant Response Capacity by Sodium Arsenite
… exposure in actively dividing and NGF treated PC12 cells. Assessment of Ogg1 activity and mRNA levels demonstrated a significant decrease for both measures subsequent to arsenite exposure. The effect seen was due in large part to alterations in gene transcription since direct testing revealed …
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Disruption of 8-hydroxy-2'-deoxyguanosine DNA Glycosylase (OGG1) Antioxidant Response Capacity by Sodium Arsenite
… exposure in actively dividing and NGF treated PC12 cells. Assessment of Ogg1 activity and mRNA levels demonstrated a significant decrease for both measures subsequent to arsenite exposure. The effect seen was due in large part to alterations in gene transcription since direct testing revealed …
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Polyethylene glycol copolymer nanocarriers: Biocompatibility, uptake and intracellular trafficking in neurons
… for its biocompatibility in a neuron model (PC12 cells), as well as in chick dorsal root ganglion (DRG) sensory neurons, cells that are damaged during SCI. We have performed time- and dose-dependent studies showing that treatment of up to 150 µg/mL PE-MNC for 72 to 96 hours does not affect …
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The growth-arrest-specific protein gas7 potentiates neuronal differentiation
… activity were investigated in PC 12 and NT2 cells. gas7 overexpression in PC12 cells promoted neurite outgrowth and potentiated nerve growth factor-induced expression of the neuronal markers betaIII-tubulin, synaptotagmin, alpha7 subunit of the acethylcholine receptor, and dihydropyrimidinase …
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Investigation of the role of neuroserpin in neurite outgrowth and learning and memory
… on neurite outgrowth was studied in NGF-treated PC12 cells. Neuroserpin knockdown was achieved by the use of short hairpin RNA (shRNA) which led to changes in the neurite extension and cell morphology of the PC12 cells. When quantitative measures of cell and neurite morphology were related to the …
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