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Showing 1 to 2 of 2 for “"PAM specificity"”.
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Generation of Memory of Infection During the CRISPR-Cas9 Immune Response
… of a 5′-NGG-3′ protospacer adjacent motif (PAM) sequence immediately downstream of the viral target. Before my graduate work, it was not known whether and how viral sequences flanked by the correct PAM are chosen as new spacers. My work revealed that Cas9 selects functional spacers by …
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Rarely Acquired Type II-A CRISPR-Cas Spacers Mediate Anti-Viral Immunity Through the Targeting of a Non-Canonical PAM Sequence
… motif, known as the protospacer adjacent motif (PAM). The PAM identifies the phage DNA as "foreign" and triggers the Cas nuclease, Cas9, to cleave the target phage DNA. Most of the spacers present in bacterial populations that survive phage infection target protospacers flanked by N-G-G PAMs. As …