Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 5 of 5 for “"PAM sequence"”.
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Rarely Acquired Type II-A CRISPR-Cas Spacers Mediate Anti-Viral Immunity Through the Targeting of a Non-Canonical PAM Sequence
… system by identifying and degrading specific sequences in invading phage nucleic acids. CRISPR loci consist of repeat sequences interspersed with short fragments of phage DNA (spacers). The cas genes code for proteins that regulate 1) the acquisition of new spacers during phage infection …
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Bacterial defense islands: CRISPR-Cas and toxin-antitoxin systems /
… by unidirectional unwinding starting from PAM sequence motif. Furthermore, the complexes can assemble on different length of crRNA and enable dsDNA targeting in a WT-like manner. The second part of the thesis is dedicated to the biochemical and structural characterization of the …
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RNA in the nucleus: insight into location and biological function
… interactions pull down individual RNAs and sequence the associated DNA. However, it remains challenging to identify chromatin associated RNAs and their genomic target sites. In this thesis, I will describe MARGI-seq, a method to identify chromatin associated RNAs and their genomic target …
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Generation of Memory of Infection During the CRISPR-Cas9 Immune Response
… in prokaryotes. Upon infection, short phage sequences known as spacers integrate between CRISPR repeats and are transcribed into small RNA molecules that guide the Cas9 nuclease to the viral targets (protospacers). Streptococcus pyogenes Cas9 cleavage of the viral genome requires the presence …