Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 13 of 13 for “"PA14"”.
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The Roles and Regulation of the Redundant Phenazine Biosynthetic Operons in Pseudomonas aeruginosa PA14
… to colony biofilm development in P. aeruginosa PA14 (Chapter 2). We found that (1) phenazine production in biofilms is mediated exclusively through the phz2 operon, (2) phz2 expression is required for biofilm development and host colonization and (3) phz2 is regulated by quinolones, which are …
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Discovery and Characterization of Small Molecule Inhibitors of Biofilm Formation in Pseudomonas Aeruginosa
… agents, I tested response of P. aeruginosa PA14 biofilms to elevated iron concentrations, utilizing nine structurally varied iron chelators as iron delivery agents in a microtiter plate assay for biofilm production. Picolinic acid and acetohydroxamic acid-chelated iron were able to stymie …
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Interactions of Fibroblast with Cytotoxic and Invasive Strains of Pseudomonas aeruginosa on ECM Mimicking Fibers
… to representative cytotoxic (PAO1) and invasive (PA14) strains of P. aeruginosa. These assays study host cell-pathogen interactions on highly aligned nanofibers of varied spacing and diameter, which mimic the fibroblast deposited ECM and dictate fibroblast morphology. We discovered that the …
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Peptidergic Signaling Controls the Dynamics of Sickness Behavior in Caenorhabditis Elegans
… the pathogenic bacteria Pseudomonas aeruginosa PA14. After a few hours of exposure to and infection by PA14, C. elegans develops a sickness-like behavior termed pathogen avoidance. Here I identify neuronal signals generated during the infection and associate them with antagonistic circuits that …
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Bacteria At Oil-Water Interfaces
… in contact with Pseudomonas aeruginosa PAO1 and PA14 suspensions. Film formation is species dependent. While PAO1 cells form elastic films, PA14 cells move actively without elastic film formation. PAO1 mutants lacking flagella, pili, or certain polysaccharides also form elastic films. …
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Influence of Indole and Mixed Culture Growth on Pseudomonas Aeruginosa Biofilm Structure
… Two wt Pseudomonas aeruginosa strains, PA14 and PAO1 were co-cultured with wt Escherichia coli BW25113 (indole producing), and E. coli tryptophanase mutant tnaA (non-indole producing). Comparably, exogenous indole was added in multiple concentrations (mM) in an effort to monitor the …
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Novel collective prey evasion response of Pseudomonas aeruginosa PAO1 to soil micropredator Myxococcus xanthus DK1622
… in another <em>P. aeruginosa </em>strain, PA14, nor in other closely related pseudomonads we tested. Transposon mutants of the quorum sensing operon <em>pqs</em> and gene <em>rsaL</em>, did not possess the fold. Here we describe a novel predation resistance response observed in PAO1 and we …
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A cystic fibrosis infection monitor
… using mutant strains of P. aeruginosa PA14 demonstrate that the changes in impedance are largely related to the production of redox active phenazine compounds, including pyocyanin. Slight changes in the impedance were observed with S. aureus and H. influenzae, but were not the same as …
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Consequences of redox-active phenazines on the physiology of the opportunistic pathogen Pseudomonas aeruginosa
… in this thesis demonstrates that P. aeruginosa PA14 can survive under anoxic conditions in the presence of glucose and oxidized endogenous phenazines, including pyocyanin, 1-hydroxyphenazine, and phenazine-1-carboxylic acid. Exogenous oxidants such as methylene blue, paraquat, and …
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The genetics and phenotypes of Pseudomonas aeruginosa biofilm dispersal populations
… CF strain LESB58, and the highly virulent PA14, due to the presence of multiple genomic islands shared between the strains. It was found that several genes were deleted in 18A and that could explain the phenotypic differences, such as poor biofilm formation compared to PAO1. These studies …
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Rapid molecular signature analysis of pseudomonas aeruginosa by direct nanomolar detection of quorum sensing and biomarker molecules at a boron-doped diamond electrode
… the production of signaling molecules of PA14 bacterial cultures was performed using CE-UV after MCX SPE. Instant feedback and detection is important in a clinical setting which is a challenging objective. Investigations into the simultaneous electrochemical detection of these microbial …
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Identification and recombinant production of antimicrobial peptides from the Black Soldier Fly, Hermetia illucens (L.)
… of P. aeruginosa including the highly virulent Pa14 strain. Membrane damage induced by the AMPs was visualised using fluorescent and transmission electron microscopy, and electrochemical impedance spectroscopy. In summary, this work has used modern and traditional approaches to identify and …
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The Dissociation of Sensory Integration and Associative Learning in Caenorhabditis elegans and the Molecular Mechanisms Leading to Memory Acquisition
The study of associative learning in C. elegans takes advantage of a genetically tractable organism with a fully mapped connectome, and with a simple nervous system compared with that of mammals. A learning deficient mutant found in a mutagenesis screen, lrn-2, was characterized and mapped to the …