Global ETD Search
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Showing 1 to 20 of 21 for “"P97"”.
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Sequential Actions of VCP/p97 and the Proteasome 19S Regulatory Particle in Sterol-Accelerated, ER-Associated Degradation of HMG CoA Reductase
… defined reactions mediated by the AAA-ATPase VCP/p97 and augmented by the nonsterol isoprenoid geranylgeraniol. Here, we report that the oxysterol 25-hydroxycholesterol and geranylgeraniol combine to trigger extraction of reductase across ER membranes prior to its cytosolic release. This …
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The p97 cofactors UBXN7 and UBXN8 interact with and modulate the function of cullin-RING complexes and Fanconi anaemia proteins FANCD2/FANCI, respectively
The ATPase p97 plays a role in diverse cellular activities such as cell cycle progression, membrane fusion, DNA repair and ubiquitin-dependent protein degradation (including endoplasmic reticulum-associated protein degradation, ERAD). The functional diversity of p97 is achieved by its association …
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Delineating the Mechanisms of Misfolded Endoplasmic Reticulum (ER) Luminal Protein Retrotranslocation for ER-Associated Degradation
… domains can be dislocated from the ER in a p97-dependent manner, but proteasome activity is required to fully release them from the cytosolic side of the ER membrane and the ERAD extraction machinery.To decipher key steps in the extraction of non-glycosylated ER luminal proteins, and to …
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The proteasome and its ancillary proteins
… I studied the proteasome interaction with the P97 AAA+ ATPase. Using the baculovirus mediated insect cell overexpression system, I recombinantly co-expressed the human P97 and the 20S proteolytic core of the proteasome. Although P97 was shown to co-purify with affinity tagged 20S proteasomes, …
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Percentiles de circunferencia de cintura en escolares de Bogotá, Colombia Estudio FUPRECOL
… los percentiles (P3, P10, P25, P50, P75, P90 y P97) y curvas centiles según sexo y edad. Se realizó una comparación entre los valores de la circunferencia de cintura observados con estándares internacionales. Resultados: De la población general (n=5.921), el 57,0% eran chicas (promedio de edad …
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Regulation of Diacylglycerol Acyltransferase-2 and Triacylglycerol Synthesis by Protein Ubiquitination
… the ERAD ATPase, valosin-containing protein (VCP/p97), where it becomes concentrated in the ER, co-localizing with both DGAT2 and MGAT2. Interaction of DGAT2 and MGAT2 with VCP/p97 was also demonstrated in situ. We took a non-targeted mass spectrometry approach to identify proteins interacting …
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Körperfettmessung bei Kindern und Jugendlichen - Alters- und geschlechtsbezogene Perzentilen von Hautfaltendicke, Taillen- und Hüftumfang sowie Taille-Hüft-Index und Taille-Größe-Index: Ergebnisse einer pädiatrischen Kohorte in Deutschland (LIFE Child)
… with disproportionately high values for P90 and P97. Boys show higher values of WC, girls have higher levels of HC. WC and HC median percentiles constantly increase in both sexes with a plateau at the age of 16 for girls. Conclusion: Trends for all parameters of body fat are in line with other …
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Molecular analysis of vesicle biogenesis during autophagy
… The human homologues of Cdc48 / Shp1 p97 / p47 are involved in human mitotic Golgi reassembly. This p97 / p47 complex is thought to extract a monoubiquitylated fusion inhibitor out of membranes and thereby mediate fusion. In this study the involvement of monoubiquitin in PMN as well as …
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Valores del índice cintura/cadera en población escolar de Bogotá, Colombia: Estudio
… los percentiles (P3, P10, P25, P50, P75, P90 y P97) según sexo y edad y se realizó una comparación entre los valores del ICC observados con estándares internacionales. Resultados: De la población general (n=5.921), el 57,0% eran mujeres (promedio de edad 12,7 ± 2,3 años). En todas las edades el …
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Mechanisms of molecular switching in the Wnt signal transduction pathway
… earmarking it for extraction by the VCP/p97 ATPase and ultimately leading to inactivation of its repressive function. In Chapter 3, I present the results of a different, ongoing study to identify the mechanism by which a cytoplasmic negative regulator, Naked, acts to interfere with the …
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Hrd1 Partners In Endoplasmic Reticulum-Associated Degradation
… and the direct interaction of HRD1 with VCP/p97, while not required for polyubiquitin chain formation by this E3 ligase, may determine some types of ubiquitin chains formed by HRD1 with either E2 conjugase. Our findings suggest that HRD1 has specific partners in different tissues with …
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Independent Functions Of Clueless In Differential Integrin Secretion And Mitochondrial Quality Control In Drosophila Muscle
… of markers for mitophagy, including Parkin, VCP/p97, p62/Ref(2)P, and Atg8a. However, clearance of damaged mitochondria is impeded. This paradox is resolved by the findings that Clu is upstream of and binds VCP and is required for VCP-dependent Marf degradation. Marf accumulates in clu-deficient …
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Using Small Molecule Tools to Study ATPase Mechanoenzymes
… activators in valosin-containing protein (VCP)/p97, a AAA unfoldase whose loss of function has been linked to protein aggregation-based disorders. For the chemical genetics approach, we designed ASPIRe-1 (Allele-Specific, Proximity-Induced Reactivity-based inhibitor-1), a cell-permeable compound …
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Biological Functions of Intracellular Hepatitis B e Antigen
… as neither wildtype nor dominant-negative p97 affected the ER-to-cytosol translocation of p22 or ER-Golgi secretion of HBeAg. Secondly, despite sharing the same sequence with core protein except for the extended 10 amino acid precore region at the N-terminus, we observed that p22 wildtype …
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Characterising the role of Valosin Containing Protein (VCP) in autophagy and cell differentiation.
Valosin containing protein (VCP)/p97 is a hexameric ATPase of the AAA family, which regulates a wide array of essential cellular processes. Dominant mutations in the N-domain of the VCP give rise to the complex disease syndrome known as Inclusion body myopathy with Paget disease of the bone and …
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Protein quality control in the mammalian endoplasmic reticulum
… glycans on the misfolded protein. UBXD8 recruits p97, the AAA+ ATPase responsible for membrane extraction of dislocated proteins, to the ER using its UBX domain. UBC6e is a membrane-anchored E2 ubiquitin conjugating enzyme. AUP1 recruits a second E2, soluble UBE2G2. Additionally, AUPI regulates …
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Mechanisms of TRIM21-directed intracellular degradation
… to lead to the recruitment of the segregase p97/VCP and the 26S proteosome which together disassembled and degraded the encapsidated virion. In this thesis, I describe my efforts to define the sequential enzymatic mechanism of TRIM21 degradative signaling in greater detail. I report data from …
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Characterization of a Novel Chromatin-Induced Mechanism that Couples Microtubule Disassembly and Nuclear Re-Formation
… the Aurora B kinase by the Triple A-ATPase Cdc48/p97, which physically extracts the protein from chromatin at anaphase. Aurora B is the catalytic subunit of the Chromosome Passenger Complex (CPC), which promotes microtubule polymerization and spindle formation from mitotic chromosomes. Removal of …
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Comparison of the therapeutic efficacy of SNAP-tag fusion protein and the synergistic actions of chemotherapy and photodynamic therapy in killing resistant melanoma
… growth factor (EGFR), melanotransferrin (p97) orfibroblast activation protein alpha (FAP-α) receptors, respectively. Achievement of this targeted therapy was performed through construction of three scFv-SNAP fusion proteins, which were expressed in HEK 293T cells. Thereafter, the purified …
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Biochemical and Structural Insights into VCP Unfoldase and WRN Helicase Complexes
<p>Valosin-containing protein (VCP or p97 in mammals, Cdc48 in yeast) is an essential member of the AAA (ATPases associated with diverse cellular activities) protein superfamily. As its family name suggests, VCP function is implicated in a broad range of biological pathways including protein …
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