Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 5 of 5 for “"P450 BM3"”.
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Towards next-generation enzymatic generators of hydrogen peroxide in quantitative redox biology
… coli, we co-expressed the sensor with cytochrome P450 BM3 in E. coli to demonstrate the efficacy of a novel whole-cell screen for H₂O₂ production. We chose P450 BM3 as the target enzyme because of its satisfaction of all criteria of an ideal H₂O₂ generator except total activity in mammalian cells. …
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Engineering novel tandem reactions using organometallic catalysts and (metallo)enzymes
… a par-ticular interest on combining cytochrome P450s and enoate reductases with organometallic cata-lysts. On the one hand, considerable efforts in transition-metal catalysis have culminated in prac-tical and efficient transformations such as isomerization, olefin metathesis, carbene-mediated …
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A quantitative analysis of chemotherapy-induced reactive oxidative species using genetically encoded sensors and generators
… enzymatic ROS generator. We explored the use of P450-BM3, an enzyme that can generate superoxide and hydrogen peroxide through a reaction that requires only NADPH and oxygen. While this reaction in the wild type protein is slow, it can be engineered to have much higher catalytic rates. We …
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Genetically engineered sensors for non-invasive molecular imaging using MRI
… on the heme domain of the bacterial cytochrome P450-BM3, which produces changes in TI contrast in MRI in response to small molecule binding. We developed a high-throughput assay that allowed us to evolve this protein into a sensor of the neurotransmitter dopamine (DA). We then used it to image …
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Engineering novel tandem reactions using organometallic catalysts and metalloenzymes
… tandem reactions between ruthenium catalysts and P450s for enantioselective and regioselective enzymatic asymmetric epoxidations, a reaction which still poses a few issues for organic chemists with respect to scope and enantioselecitivty. In Chapter 2, we combined a ruthenium isomerization …