Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 13 of 13 for “"P301L"”.
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Cell death of reelin secreting nNOS positive GABAergic interneurons in the hippocampus of P301L trangenic mice
… to chromosome 17) 질환 모델로서 Tau 단백질의 과인산화를 유도한 P301L 생쥐를 이용하여 해마 (hippocampus)에서 reelin과 nNOS를 공동으로 발현하는 GABA성 신경세포의 세포사멸에 대해서 알아보고자 했다. 이중면역조직화학법을 이용하여 P301L 생쥐의 해마에서 nNOS를 발현하는 신경세포가 GABA성 신경세포의 한 종류라는 것을 GABA의 마커인 GAD67을 통하여 확인하였으며, nNOS 양성 GABA성 신경세포에서 reelin을 발현하고 있는 신경세포들을 확인하였다. 이러한 …
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Fas-associated factor 1 is involved in neurofibrillary tangle-mediated cell death of basal forebrain cholinergic neurons in P301L transgenic mice
… 12 I. FTDP-17 13 J. Cholinergic dysfunction in P301L transgenic mice 14 Ⅱ. MATERIALS AND METHODS 16 1. Animals 16 2. Antibodies 16 3. Immunohistochemistry 16 4. Thioflavin-S staining 17 5. Sarkosyl fraction of Tau protein and immunoblotting 17 6. Stereological analysis 18 7. Statistical analysis …
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Investigating the effects of tau mutations in induced pluripotent stem cell-derived neurons
… or affect tau’s biochemical properties (such as P301L and V337M). In this project, I differentiated induced pluripotent stem cells (iPSCs) derived from control subjects and people with the N279K or V337M MAPT mutations to monolayer neurons. There was an increase in the proportion of neurons …
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Glutamate Dysregulation in a TauP301L Mouse Model of Alzheimer's Disease
… Here, we used a tau mouse model of AD (rTg(TauP301L)4510) to examine whether mutant P301L tau expression alters synaptic glutamate regulation in the dentate gyrus (DG), CA3 and CA1 regions of the hippocampus. To examine glutamate regulation in vivo, we used an amperometry coupled to …
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Mechanistic Investigation into the Regulation of Amyloid Motifs in Tau Aggregation and Disease
… biophysical mechanisms behind aggregation of the P301L/S and S320F mutants. We conclude that the aggregation prone 306VQIVYK311 motif forms metastable compact structures with its upstream sequence that modulates aggregation propensity. We report that disease-associated mutations such as P301L/S at …
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p53 aggregation, interactions with tau, and impaired DNA damage response in Alzheimer's Disease
… p53 oligomers can also be detected in htau, P301L, and Tg2576 mouse models. Additionally, we demonstrate that p53 interacts with tau, specifically tau oligomers, in AD brain and can be recapitulated by in vitro exogenous tau oligomer treatment in C57BL/6 primary neurons. p53 oligomers also …
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Neuronal Protection by a Novel C-terminal Hsp90 Modulator
… JNPL3 and rTg4510, which both contain the P301L Tau mutation and develop aberrant Tau tangles as well as cognitive impairments, we assessed KU-32 treatment in vivo. The JNPL3 mice were treated with 20 mg/kg of KU-32 or with 12.5% Captisol® (vehicle) five times a week for six months. …
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Multi-omics mapping of the stress load on cell-specific glucocorticoid-dependent cascades in Tau brain pathology
… κύτταρα (microGRKO) διαγονιδιακών ποντικών P301L-Tau υπό συνθήκες χρόνιου στρες, πραγματοποιήσαμε ποσοτική πρωτεωμική ανάλυση χωρίς σήμανση στον ιππόκαμπο και αλληλούχιση RNA (RNA-sequencing) στον προμετωπιαίο φλοιό. Στα ζώα με άθικτους υποδοχείς GR, το χρόνιο στρες οδήγησε σε καταστολή της …
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Disambiguating the Similar: Investigating Pattern Separation in Medial Temporal Lobe Structures Using Rodent Models
… neurons. Finally, it is shown that the $TgTau^{P301L}$ mouse model of dementia exhibits spatial and object recognition memory impairments once aged, recapitulating a dementia-like phenotype. Understanding the mechanisms that contribute to effective pattern separation may help elucidate the …
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De-Risking PET Radioligand Discovery for Neurodegenerative Diseases
… density compared to controls within females. In P301L mice, GSK-3 density in males was elevated compared to control, and a trend was recapitulated by in vivo PET, while females remained unchanged by both methods. GSK-3 PET research could inform the biological role of this critical kinase in …
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Investigation of physical principles of protein phase transitions
… step in facilitating aggregation. In the P301L variant of tau, I track individual tau molecules and monitor nucleation and elongation events inside condensates using total internal reflection fluorescence microscopy and spinning disk super resolution by optical pixel reassignment …
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Axonal Damage in Repetitive Concussive Traumatic Brain Injury: Characterization and Contributing Factors
… AD mutations (PS1<sub>M146V</sub>, tau<sub>P301L</sub>, and APP<sub>SWE</sub>). The Apolipoprotein E4 (APOE4) genotype is a risk factor for poor outcome following traumatic brain injury, especially in young patients. By analogy to APOE4's effects on the risk of Alzheimer's disease, one …