Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 20 for “"P2X7"”.
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Identifizierung regulatorischer Proteindomänen der zytolytischen Eigenschaften des humanen P2X7-Rezeptors
The P2X7-receptor is a ligand-gated ion channel of the P2X-purigenic-receptor group. In different of the other receptors of the P2X-family the P2X7-receptor is able to build both an ion channel and a cytolytic-pore which leads to membrane-blebbing. The aim of this MD-thesis was to develop a method …
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The role of nucleotides and the P2X7 receptor in radiation induced bystander effect
… therapy. Additionally, the purinergic receptor P2X7 was targeted in order to study the effects of inhibiting this receptor on the bystander effect Method: The cell line UVW/NAT was used in these experiments. One group of cells were directly irradiated by external beam radiation and designated …
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Roles of the P2X7 receptor in C6 astroglioma: in vitro and in vivo studies
The purinergic P2X7 receptor (P2X7R) is an ionotropic adenosine triphosphate(ATP) receptor which is closely linked with pathological conditions in the central nervous system (CNS). Gliomas are the most common primary brain tumors with presently no cures. The roles of the P2X7R in these diseases …
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The role of the P2X7 receptor in the intestinal inflammatory response to the parasite, Toxoplasma gondii
… tested in this thesis was that the purinergic P2X7 receptor plays a critical role in intestinal inflammation. This was tested by infecting mice with 10 cysts of the ME49 st rain of T. gondii - three strains of mouse were used: BALB/c mice, which are known to be resistant to toxoplasmic ileitis; …
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Charakterisierung von purinergen Rezeptoren an kultivierten Podozyten der Maus
… RT-PCR wurde die mRNA von P2Y1, P2Y2, P2Y6, und P2X7 Rezeproren nachgewiesen. <br>Die funktionelle Aktivität der Rezeptoren P2Y2 und P2Y6 konnte aufgezeigt werden. Der P2Y1-Rezeptor wies keine Aktivität auf, die Ergebnisse für den P2X7-Rezeptor waren uneinheitlich. Die Aktivierung der …
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Extracullular Atp Regulates Il-1beta Release From Microglial Cells Via Purinergic Receptor After In Vitro Trauma
… release was blocked completely by the P2X7 receptor antagonist, oxidized ATP, and partially by the P2X7 receptor antagonist suramin, suggesting that ATP stimulates interleukin-1Beta release from microglia via purinergic receptor and the P2X7 receptor is responsible for the …
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Caractérisation du rôle du récepteur P2X7 dans le transport du glucose par les cellules épithéliales intestinales et le contrôle de la glycémie et du métabolisme
Dans l’intestin, le récepteur P2X7, un membre unique de la famille P2X, est fortement exprimé à la surface des cellules épithéliales intestinales le long des villosités, ce qui suggère un autre rôle pour ce récepteur que l’induction de l’apoptose des cellules à l’apex des villosités. Dans des …
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Recettori purinergici ionotropi e i loro ruoli nella neuroinfiammazione mediata dalla microglia
… i recettori ionotropici purinergici P2X4 e P2X7. Quest'ultimo è stato ampiamente studiato come attore principale nella risposta infiammatoria mediata dalla microglia. Tuttavia, poiché i livelli extracellulari di glutammato sono aumentati in modo simile nella neuroinfiammazione e il …
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Investigation of Interleukin 1B synthesis and secretion by dendritic cells: Interplay between toll-like receptor and FCG receptor
… from moDCs. The increase was mediated through P2X7 receptor-dependent Ca2+ influx. In addition, Rac1, JNK and MEK were identified as key molecules in LPS/imIgG-induced IL-1I? production and secretion by moDCs, whilst PI3-K and p38 MAPK as negative modulators.
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Direct neuronal and microglia-mediated effects of group I metabotropic glutamate receptors in neurodegenerative and neuroinflammatory processes
… their formation occurs following activation of P2X7 purinergic receptors. We observed MVs development in pure microglial cultures, but, in the present study, they were more easily examined in the murine BV2 cell line. Activation of mGlu5 receptor attenuated the levels of pro-inflammatory …
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Einfluß von extrazellulären Nukleotiden auf den eosinophilen Granulozyten
… spezifischer mRNA der Subtypen P2X1, P2X4, P2X7, P2Y2, P2Y4, P2Y5, P2Y7 und P2Y11 erbracht werden. <br>Weiter konnte gezeigt werden, daß ATP, UTP und BzATP konzentrationsabhängig eine Mobilisation von Kalzium in Eosinophilen verursachen. Die Expression des Adhäsionmoleküls CD11b nahm nach …
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Investigating the role of the CCL2/CCR2 axis in monocytes and its modulation by extracellular nucleotides
… also indicated that although P1, P2X1, P2X4, P2X7, P2Y1, P2Y11, P2Y12, and P2Y13 purinoceptors were unlikely to play a major role, P2Y6 and CCR2 engaged in crosstalk. Cross-desensitisation experiments and studies on P2RY6-knockdown THP-1 cells supported this finding and indicated that a …
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Increased Microglial Reactivity Alters Morphine Analgesia
… explores the contributions of the microglial P2X7 receptor and the microglial transcription factor Runx1 in morphine analgesia and in the development of adverse effects. My over-arching hypothesis is that increased microglial reactivity diminishes the analgesic potential of opioids, such as …
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Novel cytokines in innate immunity: IL-27 and IL-30 enhance responsiveness to gram-negative bacterial components in human myeloid cells
… in human monocytes via upregulated TLR4 and P2X7 expression. Comparing human THP-1 monocytes and PMA-differentiated THP-1 macrophages, CD14 expression plays a role in LPS and IL-27 responsiveness, whereby IL-27-mediated inhibition of LPS tolerance is greater in CD14low THP-1 monocytes …
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IMPACT OF KETOGENIC DIET TREATMENT IN GLUT1 DEFICIENCY SYNDROME: DISCOVERY OF MOLECULAR MECHANISMS RESPONSIBLE FOR PHENOTYPE CHANGES IN BOTH PEDIATRIC AND ADULT PATIENTS
… codificanti canali ionici, tra cui il recettore P2X7, strettamente connesso al gene SLC2A1 e a processi neuro-infiammatori. Questo dato è stato confermato anche dall’analisi del metiloma che mostra pattern di metilazione alterati in geni canale, come GRIN3A. I risultati di questo studio, nuovi e …
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Amyotrophic Lateral Sclerosis: mechanism behind mutant SOD toxicity and improving current therapeutic strategies
… motor neuron death through activation of the P2X7 receptor. Motor neuron death required phosphatase and tensein homolog (PTEN)-mediated inhibition of the PI3K/AKT pathway leading to Fas receptor activation and caspase dependent death. The relevance of Hsp90 for motor neuron survival was …
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Generation of a novel pan-cancer chemokine receptor-modified CAR-T cell therapy directed towards nfP2X7
… directed towards the nonfunctional form of the P2X7 receptor (nfP2X7), which is an ATP receptor expressed by a diverse range of malignant cells that is completely absent on healthy tissues. This is the first use of nfP2X7 as a target antigen for CAR-T cell therapy and represents one of the first …
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New purinergic-based pharmacological targets for the treatment of retinal disorders
… purinergic signaling pathway, which includes the P2X7 receptor (P2X7R). This ATP-gated ion channel has been linked to vascular inflammation due to an over-expression of cytokines, contributing to BRB alteration. The integrity of this physical barrier is essential for a proper vision; indeed, the …