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Showing 1 to 20 of 34 for “"P. pastoris"”.
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Calcium homeostasis in Pichia pastoris
<p>Pichia pastoris is a methylotrophic yeast that has been used widely in biological and industrial researches. P. pastoris is able to produce from milligram to gram quantities of protein. In this study, we examined the effects of calcium and magnesium on growth, heterologous protein expression, …
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Identification and characterization of components that overcome secretion limitations of the yeast Pichia pastoris
<p>The methylotrophic yeast. Pichia pastoris, is a powerful, adaptable, and inexpensive recombinant expression system commonly used to secrete heterologous protein. Although P. pastoris is a popular host organism, secretion inefficiency continues to be a major hurdle in its ability to produce high …
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Cloning and characterization of the Pichia Pastoris PMR1 gene
<p>Pichia pastoris, a popular protein expression system, is limited in its ability to secrete heterologous proteins. The PMR1 gene, the disruption of which is known to improve the secretion of prochymosin, human prourokinase, and human tissue plasminogen activator in Saccharomyces cerevisiae, was …
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Development of a high throughput reporter system using β-Galactosidase in the yeast : Pichia Pastoris
<p>Pichia pastoris is a methylotrophic yeast gaining acclamation for its capabili ties in heterologous protein expression. In contrast to other host organisms such as bacteria or mammalian cells, P. pastoris offers many advantages over its counterparts. For example, P. pastoris is cost-effective in …
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Bioengineering the Expression of Active Recombinant Human Cathepsin G, Enteropeptidase, Neutrophil Elastase, and C-Reactive Protein in Yeast
<p>The yeasts Pichia pastoris and Kluyveromyces lactis were used to express several recombinant human proteins for further biochemical characterization. Two substitution variants of recombinant human enteropeptidase light chain (rhEPL) were engineered to modify the extended substrate specificity of …
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Design of a cultivation medium for protein production in Pichia pastoris based on genome-wide biological understanding
… One promising alternative host is Pichia pastoris, a methylotrophic yeast that is currently used to manufacture ten approved drugs worldwide. Its fast growth rate and ability to grow to high cell densities can enable fast production cycles, agile process development, and potentially low …
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Cloning and characterization of MET2 in Pichia pastoris
<p>The methylotrophic yeast, Pichia pastoris, has been used as a protein expression system to express over 500 heterologous proteins. P. pastoris provides many advantages over other organisms that have been utilized for this purpose. In this project, we developed a new host/selectable marker and …
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Structural characterization of the MATα prepro-peptide secretion leader in Pichia pastoris
<p>The methylotrophic yeast, Pichia pastoris, is the most successful and favored microbial eukaryotic expression system for the production of recombinant proteins for biopharmaceutical or industrial purposes. P. pastoris has the ability to produce foreign proteins at high levels extracellularly, …
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Characterization of the MATα pre-/pro- peptide by mutagenesis as a means to optimize secretion in pichia pistoris
<p>The unicellular yeast, <em>Pichia pastoris</em> has currently emerged as one of the most popular host systems for heterologous proteins due to its relatively cheap cost, easy genetic manipulability, ability to perform post-translational modifications on proteins, and respiratory growth …
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Characterization of components that increase secretion of recombinant proteins in pichia pastoris
<p>Pichia pastoris is a methylotrophic yeast that is commonly used for its ability to express and secrete heterologous proteins. However, some proteins are not readily secreted in P pastoris and so adjustments in the secretion pathway must be made in order to achieve secretion. The Lin-Cereghino …
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Συνέκφραση και μελέτη υπομονάδων του νικοτινικού υποδοχέα ακετυλοχολίνης ανθρώπινων νευρικών κυττάρων
… τη βοήθεια βακιλοϊού, όσο και σε ζυμομύκητες P. pastoris, σε δυο μορφές: Τον άγριου τύπου υποδοχέα (ΑΤ) και μια μορφή χωρίς κυτταροπλασματική θηλιά (Κ). Στόχος της παρούσης μελέτης, ήταν η έκφραση ενός τύπου α4β2 nAChR με την καλύτερη δυνατή ποιότητα δομής και τα υψηλότερα δυνατά επίπεδα …
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Study of the aryl hydrocarbon receptor as a target for rational drug design
… D2 inhibitory molecule. In chapter 2, a Pichia Pastoris expression system was constructed expressing codon optimized human full length AhR. This codon optimization is necessary for overexpression of huAhR. RT-PCR data showed that the codon optimized mRNA was more stably expressed than wild …
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Clonal selection and characterization of epigenetic variation in Pichia pastoris
… the heterologous protein production host Pichia pastoris, which resulted in overall improvement of the secretion rate of the mutated population. More than 4000 single-cells were simultaneously screened for high secretion of a human Fc fragment using microengraving and the top-producing clones …
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Analysis of a trichomonas vaginalis cysteine protease
… cysteine protease (CP1). E. coli and P. pastoris expression systems were developed to produce CP1 to generate antiserum, and to have enough active protein for biochemical characterization. Secondly, endogenous and epitope tagged CP1 were localized in T. vaginalis vesicles. These vesicles …
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Herstellung und Charakterisierung von antimykotisch wirksamen Peptiden aus der AFP-Familie der Ascomycota
… gebildet wird. Die heterologe Expression in P. pastoris ist daher der geeignetste Ansatz für die AFP Produktion, die homologe Herstellung in A. giganteus ermöglicht die Funktion anderer AFP-Varianten aufzuklären. Im zweiten Teil der Arbeit wurde der Einfluss der C3-Sättigung von …
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Manufacturing GMP-grade mRNA vaccine-associated Enzymes: Bioprocess Development and Economics
… between Escherichia coli (E. coli) and Pichia pastoris (P. pastoris) was performed to evaluate the economic significance of microbial platform choice. The estimated costs of production and CAPEX requirements suggest that a viable and sustainable local enzyme manufacturing industry is possible, …
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Characterizing potential secretion components that increase secretion of recombinant proteins in Pichia Pastoris
<p>The methylotropic yeast Pichia pastoris has been used for many applications, particularly for its ability to produce and readily secrete heterologous proteins. Nonetheless, there are obstacles in making this useful yeast into a more efficient secretion system that readily secretes problem …
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Characterization of the 5̕ untranslated region ( 5̕ UTR) of the alcohol oxidase I (AOX I) gene in Pichia pastoris.
… of the Alcohol Oxidase I (AOXI) gene in Pichia pastoris. The 5' UTR influences the expression of many heterologous proteins in P. pastoris. However, no systematic analysis has ever been performed on this region to date. Several truncated versions of the 5' UTR were constructed using the …
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Biosynthesis of Cucurbita maxima trypsin inhibitor I in the methylophic yeast Pichia pastoris
… shortcomings, the methylotrophic yeast Pichia pastoris was investigated as an alternative means of expression, although at the inception of this work, no disulphide-rich proteins of this size had been expressed in P. pastoris. It was a challenge to investigate the feasibility of producing …
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Biosynthesis of Cucurbita maxima trypsin inhibitor I in the methylotropic yeast Pichia pastoris
… shortcomings, the methylotrophic yeast Pichia pastoris was investigated as an alternative means of expression, although at the inception of this work, no disulphide-rich proteins of this size had been expressed in P. pastoris. It was a challenge to investigate the feasibility of producing …
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