Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 41 for “"P-selectin"”.
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The effect of P-selectin pattern width on HL-60 cell rolling behavior
The effect of varying the width of P-selectin band patterns on the rolling behavior of HL- 60 myeloid cells along edges of the band patterns was studied. The P-selectin and polyethylene glycol (PEG) pattern was produced via microcontact printing using a polydimethylsiloxane (PDMS) stamp. HL-60 …
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Isolation and engineering of a high affinity antibody against P-selectin glycoprotein ligand-1 (PSGL-1)
We aim to develop novel protein antagonists of P-selectin adhesion as anti- inflammatory therapeutics. Blocking P-selectin adhesion is particularly attractive because this adhesion mediates leukocyte rolling which occurs early in the inflammatory cascade before extensive tissue damage caused by the …
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APPROACHES TO IDENTIFY SURFACE PROTEINS OF ANAPLASMA PHAGOCYTOPHILUM DENSE-CORED ORGANISMS AS ADHESINS TO HUMAN P-SELECTIN GLYCOPROTEIN LIGAND-1
… anaplasmosis. Sialyl Lewis x (sLex)-modified P-selectin glycoprotein ligand-1 (PSGL-1) is the confirmed receptor utilized by A. phagocytophilum to bind and invade human neutrophils and myeloid cell lines. As an obligate intracellular pathogen, the binding of A. phagocytophilum to a host cell …
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On Microvascular Inflammation and Intestinal Leakage in Radiation Enteropathy
… It was found that immunoneutralization of P-selectin (CD62P) and P-selectin glycoprotein ligand-1 (CD162) abolished radiation-provoked leukocyte and platelet rolling in the colon. Moreover, inhibition of P-selectin and P-selectin glycoprotein ligand-1 also markedly decreased firm adhesion of …
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Charakterisierung des proinflammatorischen Phänotyps neointimaler glatter Gefäßmuskelzellen nach Gefäßverletzung
… by a constitutively enhanced expression of P-selectin, GRO-alpha, SDF-1alpha;, fractalkine and MCP-1. Unlike fractalkine, GRO-alpha; and SDF-1alpha; MCP-1 – albeit upregulated in neointimal SMCs – was not immobilized on the surface of neointimal SMCs. Increased expression of P-selectin in …
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Rapid 3-D laser microprinting of bioscaffolds and patterning of proteins
… containing microprinted patterns of P-selectin, a protein involved in leukocyte recruitment from blood vessels. We showed that leukocyte rolling occurred on P-selectin patterned collagen channels. Second, we presented a 3-D printed microvasculature by seeding endothelial cells into a …
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The role of platelets and their associated recruitment mechanisms, in intestinal ischaemia reperfusion injury
… a dual therapy that targeted both GPIb and P-selectin. The strongest anti-platelet effect was observed with blocking the IIb3 integrin, but this was also associated with a sustained bleeding from the mucosal surface. Overall, the research within this thesis suggests that therapeutic strategies …
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A Microfluidic Approach For Investigating The Role Of Blood Flow In Thrombosis
… exhibited core-shell architecture with a P-selectin-positive, fibrin-rich core and a P-selectin-negative outer shell. VWF was present throughout the clot structure at both low and high shear rates, but platelet and VWF deposition was markedly decreased when N-acetylcysteine was added. In …
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Dynamics of Endogenous Cardiac Repair and Methods for Enhanced Post-Injury Cell Therapy
… BMC phenotypes. Results show that endothelial P-selectin surfaces at 30 minutes post ischemia-reperfusion injury, leading to preferential engraftment of c-kit+ BMCs (which exhibit superior P-selectin adherence in vitro). Chapter 5 adapts the flow cytometry technique to measurement of absolute …
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Platelets : their exploitation and elimination
… to the metastatic process of platelet P-selectin and endothelial P-selectin. (ii) The determinants and mechanisms of antibody-mediated platelet elimination, as seen in the medical condition known as immune thrombocytopenic purpura (ITP), and the role of inhibitory self-recognition systems …
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Platelets : their exploitation and elimination
… to the metastatic process of platelet P-selectin and endothelial P-selectin. (ii) The determinants and mechanisms of antibody-mediated platelet elimination, as seen in the medical condition known as immune thrombocytopenic purpura (ITP), and the role of inhibitory self-recognition systems …
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Disabled-2 regulates platelet heterotypic and homotypic aggregation through sulfatide binding
… stimulates increased αIIbβ3 integrin and P-selectin expression through stimulation of continued platelet degranulation, and these surface receptors mediate platelet heterotypic and homotypic aggregation. Here, we show that Dab2 N-PTB binding of sulfatides serves to increase the inhibitory …
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Role of Monocytes and Neutrophils in Thrombosis
… involved and the interaction between platelet P-selectin and its receptor PSGL-1 found on monocytes was identified as both necessary and sufficient to induce this event. The process was successfully inhibited with P-selectin and PSGL-1 blocking antibodies and could be induced in isolated …
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Development of biomimetic microfluidic adhesive substrates for cell separation
… HL60 cells as a model leukocyte cell line and P-selectin as a model receptor. Settling distance of HL60 cells under different shear stresses inside microfluidic channels was identified from the study of convection velocity of cells at different position along the channel. The results show that …
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On Leukocyte Recruitment in Cholestatic Liver Injury
… and secretion. In doing so, it was found that P-selectin-mediated recruitment of leukocytes, but not the local production of pro-inflammatory mediators, is the primary cause of sepsis-associated cholestasis. Moreover, obstructive cholestasis is associated with P-selectin-mediated intrahepatic …
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On the role of mast cells in chemokine-induced leukocyte recruitment
… can activate endothelial cells to express P-selectin and CXC chemokine (MIP-2) and cause leukocyte adhesion in vitro in a glucocorticoid-sensitive manner. Interestingly, chemokines, both MIP-Ialpha, MCP-I and MIP-2, KC, were found to provoke a dose-dependent increase in P-selectin-dependent …
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The effect of freeze dried whole watermelon powder on endothelial function and vascular health in older adults
… high-density lipoprotein, ICAM-1, VCAM-1, and P-selectin from baseline to final visits. These findings suggest that consumption of watermelon may reduce the risk or progression of cardiovascular disease from improved lipid and vascular biomarkers.
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Chemomechanics at the cell-material interface : measurements and implications of forced molecular unbinding
… cells rolling on endothelial cell surfaces and P-selectin-conjugated glass substrata.
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Recombinant Mucins with Tailored Glycosylation as Bacterial Toxin Inhibitors
… with tailored glycosylation by expressing P-selectin glycoprotein ligand-1/mouse immunoglobulin G2b (PSGL-1/mIgG2b) together with glycosyltransferases that are known to mediate the biosynthesis of specific carbohydrate determinants. PSGL-1/mIgG2b, which we have proposed as a versatile …
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