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Showing 1 to 20 of 29 for “"P-element"”.
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Mechanisms governing transposable element expression in the Drosophila germ line
Transposable element activity represents a critical threat to host organisms due to its potential to damage the genome and disrupt gene expression. Transposons are known to be particularly active in the developmental context of the germ line, to fulfil their selfish drive to increase in copy number …
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Population genetics of P transposable elements and their host species, with emphasis on Drosophila willistoni and Drosophila sturtevanti
The evolution of the P element family was studied in members of the Drosophila willistoni and Drosophila saltans species groups (subgenus Sophophora). The transmission of P elements among species, their spread within species and the strength of selective constraints, as well as the level at which …
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OMB and ORG-1
… and followed a reverse genetic strategy . All P element lines cytologically mapping to 7E-7F were characterized for their precise insertion sites. 13 of the 19 analyzed lines had P element insertions within a hot-spot 37 kb downstream of org-1. No P element insertions within org-1 could be …
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Understanding Host Tolerance to Transposable Elements
Transposable elements (TE) are mobile genetic parasites, whose unregulated activity in germline causes DNA damage, and results in sterility. Host genomes can avoid these fitness costs of TEs either by regulating TE proliferation, or by altering gametogenesis to tolerate TE-induced DNA damage. …
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The genetic and molecular analysis of the mfs(2)31 locus in Drosophila melanogaster : a novel suppressor of position-effect variegation
… one of whichwas isolated in a screen for P element insertions. The new alleles of mfs(2)31 were used for a phenotypic analysis of the locus. Strong alleles that were lethal as homozygotes died in the larval phase, while weaker alleles exhibited the previously described bristle, sterility and …
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Chromosome malorientation precedes nondisjunction during female meiosis I
… alleles generated by imprecise excision of a P element in the 5’ UTR were compared through a genetic nondisjunction assay and cytological examinations for the chromosomes' coorientation. These excision mutants have been shown to be competent to complete congression, but produce varying levels of …
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Responses to transposon activity and genome damage during germline development
… where genetic conflicts between selfish genetic elements, like transposons, and the host genome take place. Excessive transposon activity fulfils the selfish drive of transposons to increase in copy number in the host genome but can impair genome integrity and functionality, thereby threatening …
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An investigation into novel molecules involved in the development of the nervous system of Drosophila melanogaster
… and otk2. An examination of a line with a P-element in the coding sequence of CG7565 revealed aberrations throughout the embryonic motor neurons. Abnormalities in these nerves were also observed in a line with a deficiency spanning CG7565, as well as in embryos misexpressing CG7565 in motor …
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Identification of a Novel Regulator of ER Stress-Induced Apoptosis
… an ex vivo assay was developed to screen 452 p-element lethal FRT lines generated by the UCLA Undergraduate Consortium in Functional Genomics. Drosophila is amenable for a modifier screen using the FLP/FRT technique, in which mitotic recombination is used to generate loss-of-function clones in …
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Genetic analysis of Polycomb group interactions in imaginal discs of Drosophila Melanogaster and transposon tagging of the Asx Gene
… the interactions I have therefore isolated a P-element induced mutation of Additional sex combs (Asx) as the first step in cloning the gene.
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Towards a Molecular Genetic Analysis of Spermatogenesis in Drosophila
… of the germ line stem cell. We next isolated P-element induced male-sterile mutants, which underwent mapping, complementation and phenotypic analyses. Three of these mutants were characterized in more detail. First, we showed that chickadee is required, presumably in stem cells, for germ cell …
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Defining the Limits and Mapping the Control Sequences of the Gene, Enhancer of Rudimentary, in Drosophila melanogaster
… with the transgene study, deletions caused by P-element excision, exclusively located in the control region of e(r) were isolated and analyzed to determine effect on gene activity. The further refined region was then used with the EvoPrinter program, which .uses the 12 other Drosophila species as …
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Mutational analysis of JIL-1, a tandem kinase in Drosophila
… (referred to as JIL-1). Studies of JIL-1 P-element deletion and insertion loss-of-function mutations suggest that JIL-1 plays a role in histone H3 serine 10 phosphorylation, polytene chromosome morphology maintenance, dosage compensation pathways, and homeotic transformation pathways. Due to …
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In vivo imaging of long-term changes in the Drosophila neuromuscular junction
… wurden. Der Screen selbst wurde mit piggyBAC / P-Element Hybrid-Transposon durchgeführt. Hierbei wurden positive Larven mit Hilfe eines automatischen Embryonen-Sorters isoliert. Des Weiteren wurde das erste Protein, das in Drosophila an die Cytomatrix der aktiven Zone lokalisiert, identifiziert …
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Molekulare und funktionelle Charakterisierung der reggie/flotillin Gene in Drosophila melanogaster
… <br>Im Rahmen dieser Arbeit wurde eine P-Element-induzierte reggie-1/flotillin-2 loss-of-function Mutante charakterisiert, bei der keine reggie-1/flotillin-2 mRNA gebildet wird. Obwohl aufgrund der evolutionären Konservierung der reggie/flotillin-Gene erwartet wird, dass diese wichtige …
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Coregulation of a Neighboring EST with RHOGAP
… from the RhoGAP88C transcriptional start). A P-element insertion P[PZ]l(3)06951 is located immediately adjacent to an EST Clot 13975, but the homozygous lethal phenotype of the insertion appears to be unrelated to this EST. The data presented by Denholm et al. (2005) showing that 1(3)06951 fails …
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Mapping And Characterization Of 18-5 And 12-5, Genes Which Potentially Link The Rhoa Signaling Pathway To The Ecdysone Response
… within the Drosophila genome utilizing a P-element recombination mapping technique. This work narrowed the location of the 18-5 locus to within an interval of 112 kb within the Drosophila genome sequence. This interval contains 17 known and predicted genes. I also mapped the location of the …
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Characterisation of the dead ringer gene of Drosophila melanogaster
… the central nervous system. The phenotype of P - element insertion and deletion mutations of dri were identified as causing embryonic lethality preceded by a disruption of the hindgut and loss of Dri expression in the ring gland. The identification of the novel, conserved DNA - binding domain in …
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Function of the forkhead gene fd3F in Drosophila chordotonal neuron differentiation
… deletion mutant by imprecise excision of a P element. Mutant adults and larvae exhibit impaired coordination characteristic of Ch neuron defects and a similar phenotype was observed in fd3F RNAi lines. fd3F mutant Ch neurons do not show gross morphological defects, however the tips of the Ch …
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Genetic characterisation of the Drosophila Mitochondrial Calcium Uniporter in physiological and neurodegenerative contexts
… melanogaster, through molecular cloning, P-element transposition, and CRISPR-mediated targeted mutagenesis. Knockouts of MCU and EMRE, both essential for in vivo uniporter activity, are largely tolerated in the fly, though lifespan is reduced, especially for MCU. Metabolic features of these …
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