Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 22 for “"P bodies"”.
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Quantitative Studies of Composition and Formation of Yeast P Bodies
… protein partitioning into condensates. Using P bodies, an archetypal biomolecular condensate that concentrate proteins and RNA, I first generated a quantitative inventory of the major proteins in yeast P bodies. I found that only 7 proteins are highly concentrated in P bodies while the 24 others …
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Investigating the function and mechanistic control of processing bodies in early Drosophila melanogaster development
… function and mechanistic control of processing bodies (P-bodies), an evolutionarily conserved RNP granule. As perceived sites of RNA metabolism, the function of P-bodies, if any, has been widely debated. I investigate P-bodies, observing and manipulating their relationship with axis-patterning …
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Relationship between Processing Body Formation, Epithelial-to-Mesenchymal Transition, and Invasion in Lung Adenocarcinoma
… TGFβ1 enhances the formation of processing-bodies (P-bodies) where P-body proteins are required for invasion in multiple cancerous cell lines. P-bodies are a type of ribonucleoprotein (RNP) granule that assist in post-transcriptional gene regulation by storing or degrading mRNAs. However, …
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Investigating processing body condensation, material properties and function during early Drosophila development
… Conserved across eukaryotes, Processing bodies (P bodies) are cytoplasmic biomolecular condensates which act as hubs for RNA regulation including storage, translation, and degradation. During Drosophila oogenesis, several maternal mRNAs are stored and translationally repressed inside P …
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SYK promotes TGF-beta-induced P-body clearance in breast cancer cells through the enhancement of autophagy
… of these mRNPs are associated with processing bodies (P-bodies), mRNP granules that are associated with mRNA degradation. These interactions led me to hypothesize that Syk co-localizes with P-bodies. I found that Syk co-localizes with P-bodies in HEK293T cells. Moreover, I show tyrosine …
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Investigating the regulation of processing bodies in Drosophila melanogaster oocytes
… degradation. Within this category, processing bodies (P bodies) are evolutionarily conserved RNP granules found in yeast to humans. In Drosophila melanogaster, P bodies play a crucial role in regulating the maternally deposited axis-determining mRNA, bicoid (bcd). In the mature oocytes, bcd is …
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Functions of Deadenylation Factors In Mrna Decay and Mrna Processing Body Formation
… dynamic cytoplasmic foci termed mRNA processing bodies, or P bodies, which are conserved in eukaryotes. To shed new light on the mechanisms of P body formation and P body functions, I focused on the link between deadenylation factors and P bodies. I found that the two major deadenylation …
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Regulation of APOBEC3 activity and HIV-1 replication by P-body associated proteins
… cytoplasmic foci termed mRNA Processing (P) Bodies. These foci have very recently been implicated in influencing viral life cycles. However, the functional relevance of the interaction with the Argonaute proteins and localisation to P-bodies, to APOBEC3 anti-viral and cellular activity is …
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Compositional Control of Phase-Separated Cellular Bodies
Cellular bodies such as P bodies and PML nuclear bodies (PML NBs) appear to be phase separated liquids organized by multivalent interactions among proteins and RNA molecules. Although many components of various cellular bodies are known, general principles that define body composition are lacking. …
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The Regulation of ARE-Mediated mRNA Decay During Colorectal Tumorigenesis
… RNA decay centers known as processing bodies (P-bodies). However, the mechanism that drives P-body assembly is largely understudied. The mRNA decay factor tristetraprolin (TTP), by virtue of its ability to bind AREs, promotes ARE-mediated mRNA decay by sequestering its target …
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Characterisation of the multi-isomeric protein nesprin-1 in p-body and mRNA dynamics
… cytoplasmic RNA granules called mRNA processing bodies (P-bodies). Using GST pull-downs and co-immunoprecipitations (co-IPs), p50Nesp1 was found to complex with mRNA decapping factors and translational repressers.<br/>More importantly, p50Nesp1 associated strongly with microtubules, both in vitro …
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Novel Functions for Neuronal RNA Processing Bodies in the Control of Axon Terminal Growth in Drosophila Melanogaster
… HPat/Pat1, a core component of RNA processing bodies or "P bodies". We show that <em>hpat</em> mutants exhibit a strong synaptic hyperplasia at the developing and acutely stimulated <em>Drosophila</em> larval neuromuscular junctions (NMJs). The synaptic defects observed in hpat mutants are …
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Stress-Induced Targeting of Molecular Chaperones In The Yeast Saccharomyces Cerevisiae
… the sHSP Hsp26 accumulate into RNA processing bodies (P- bodies) and stress granules, sites of mRNA metabolism. I found that Hsp104 recruits both Ssa1 and Hsp26 to P-bodies and that these three chaperones are required for stress granule formation. These findings suggest a possible role for …
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IAP antagonist Grim mediates cell death through divergent pathways
… Grim to distinct cytoplasmic granules, i.e. P-bodies, where translation is repressed due to the accumulation of protein components that regulate mRNA translation or stability. Knockdown of a P-body-localized translational repressor, Me31B, largely reduced IBM-independent cell death. Taken …
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Affect before Spinoza: Reformed Faith, Affectus, and Experience in Jean Calvin, John Donne, John Milton and Baruch Spinoza
… exceed, reconfigure and reorganize </p><p>bodies and subjects. Affects are constitutive of and integral to dynamic economies of </p><p>activity and passivity. This dissertation traces the origins and histories of this definition </p><p>of affect, from the Latin affectus, discovering …
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mRNA localization in Caenorhabditis elegans embryogenesis
… well-studied mRNA foci, such as germ granules, P-bodies, and stress granules, do not follow the lock-and-key principle of stoichiometric complex formation, but are actually phase-separated, biomolecular condensates. Due to their liquid-like nature, biomolecular condensates can aggregate or …
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The effect of Coxsackie virus A9 infection on nuclear and nucleolar proteins
… RNA-binding proteins e.g. stress granules and P-bodies. They also do not correspond to CAV-9 replication complexes. Two other paraspeckle proteins (PSF and NONO) colocalise with PSPC-1 in these structures. The effect does not seem to be due to cleavage of these proteins by virus proteases, …
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A Cell Biological Determination of Integrator Subunit Localization
… discrete subnuclear localization (i.e. to Cajal bodies) of any of the subunits could provide important clues to the function of that subunit. In this study, we used a cell biological approach to determine the localization of the 14 Integrator subunits. <strong>We hypothesized that the majority of …
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Destabilization of IL-8 mRNA by Anthrax Lethal Toxin: Demonstration of the Requirement for TTP and Examination of its Cellular Interactions
… also increased the colocalization of TTP to P-bodies, cytoplasmic sites concentrated with RNA decay enzymes. This suggests that the post-translational modification of TTP induced by LeTx led to its enhanced destabilization function. Identified TTP-associated proteins, non-muscle myosin heavy …
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Computational and experimental detection of uncoupling between transcriptome and translatome changes of gene expression
… is proposed and the involvement of P-bodies and the miRNA pathway in post-transcriptional reprogramming of gene expression has been experimentally tested in the biological model of EGF induction, in order to explain how a change in translational fitness can counteract or magnify a …
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