Global ETD Search
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Showing 1 to 20 of 20 for “"Oxysterols"”.
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Anti-Inflammatory Mechanism By Oxysterols In Cultured Rat Brain Astrocytes
… are enriched in the brain and can be oxidized to oxysterols by spontaneous or enzyme-mediated processes. Oxysterols are transport forms of cholesterols across cell membranes and the blood-brain barrier. Here, to elucidate the roles of oxysterols in brain inflammation, I treated …
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Oxysterols in Neurodegeneration and the Influence of Presynaptic α-Synuclein Phosphorylation
… of oxidised cholesterol derivatives known as oxysterols. Notably, 24S-hydroxycholesterol (24S-HC) and 27-hydroxycholesterol (27-HC) have been implicated in exacerbating neurodegenerative processes by modulating protein aggregation and increasing cellular vulnerability. However, the precise …
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Harmful effect of oxysterols in the gut and at systemic level: modulatory activity of olive oil phenolics
… introduce harmful substances. Among others, the oxysterols are particularly relevant; they can be absorbed from the diet and are the oxidized products of cholesterol present in cholesterol-containing foodstuffs after cooking, processing and storage, such as meat, fish, dairy products and eggs. …
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The Signaling Pathway of Oxysterol-Induced Apoptosis in Macrophages.
… events associated with atherosclerosis. Oxysterols, the major cytotoxic component of OxLDL, induce apoptosis in macrophages by a calcium flux mediated activation of cytosolic phospholipase A<sub>2</sub> resulting in the release of arachidonic acid (AA). Inhibition of AA metabolism has …
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An Examination of Oxysterol Effects on Membrane Bilayers Using Molecular Dynamics Simulations
… I focus on a group of small molecules called oxysterols, oxygenated derivatives of cholesterol. They are present in small concentrations in mammalian cells and have been shown to be involved in multiple regulatory pathways involving control of cellular cholesterol levels. The mechanisms …
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Effetto pro-ossidante e pro-infiammatorio degli ossisteroli in cellule intestinali: attività modulatoria della frazione fenolica del vino
… oxidizing agents cholesterol oxidation products, oxysterols, are particularly relevant; besides being generated endogenously, they may be introduced with the diet. Oxysterols are involved in physiological processes, but they also exert several detrimental effects including cytotoxicity, induction …
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Mammalian cell cultures as a model system for the determination of cytotoxicity induced by cholesterol oxidation products
Oxysterols are products of cholesterol oxidation, which may be produced endogenously or may be absorbed from the diet where they are commonly found in foods of animal origin. Oxysterols are known to be cyctotoxic to cells in culture and mode of toxicity has been identified as apoptosis in certain …
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Purification of Native and Recombinant NPC1
… binds 25-hydroxycholesterol (25-HC) and other oxysterols. Described here is the initial purification of rabbit NPC1 using a classical biochemical approach and an analysis of the sterol binding properties of native and recombinant NPC1. Our purification yielded a membrane-bound 25-HC-binding …
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Studies on Bioactive Lipid Mediators Involved in Brain Function and Neurodegenerative Disorders. The effect of ¿-3PUFA supplementation and lithium treatment on rat brain sphingomyelin species and endocannabinoids formation; changes in oxysterol profiles in blood of ALS patients and animal models of ALS.
… endocannabinoids (EC). These lipid mediators and oxysterols elicit potent bioactive functions in many physiological and pathological processes of the brain and neuronal tissues. They have been investigated for biomarker discovery of ageing, neuroinflammation and neurodegenerative disorders. The …
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Sterol Binding to Niemann-Pick Type C1 Disease Protein (NPC1) - Implications for its Function in Cholesterol Transport
… binds oxygenated metabolites of cholesterol, oxysterols. We then showed that NPC1‘s sterol binding domain was localized to its N-terminal luminal soluble domain (NTD) and that this domain bound cholesterol as well. We first attempted to understand the functional significance of NPC1 binding to …
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CHOLESTEROL INDUCED GENE EXPRESSION CHANGES IN THE BRAIN AND CEREBRAL VESSELS
… regions. Acute intracortical administration of oxysterols at low concentration was more potent in inducing gene expression alterations than cholesterol. In contrast, consumption of a high-cholesterol diet for an extended period chronically induced massive gene expression changes in the brain and …
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ROLE OF HIGH DENSITY LIPOPROTEINS AGAINST PROSTATE CANCER
… Cholesterol can also be converted into oxysterols, endogenous LXR ligands that generally exert antiproliferative effects. However, 27-hydroxycholesterol (27-HC) has been shown to promote tumor growth in breast cancer, although its role in PCa remains controversial. Several studies report …
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Molecular and cellular aspects of the SREBP pathway
… by targeted inhibition of SREBP processing by oxysterols. Statins have significant potential as anti-cancer agents as they inhibit the activity of HMGCR leading to a deficiency in mevalonate which is essential for cell survival. The levels of HMGCR fluctuate widely due to cholesterol feedback …
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Lipid Regulation as a Critical Factor in the Development of Alzheimer's Disease
… cells develop this dysregulation when exposed to oxysterols. Finally, I developed and characterized a method of quantifying these detrimental changes using a fluorescence compound, filipin, which could form the basis of a commercial test to evaluate the potential risk of conversion from mild …
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Synthesis and cytotoxity of oxysterols:studies on the A,B ring polyoxygenated sterols
Oxysterols (OS), the polyoxygenated sterols, represent a class of potent regulatory molecules for important biological actions. Cytotoxicity of OS is one of the most important aspects in studies of OS bioactivities. However, studies, the structure-activity relationship (SAR) study in particular, …
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Defining the genetic and molecular pathways underlying motor neuron degenerative diseases
… development of methodologies to quantify oxysterols in small mitochondria-ER subcellular fractions of blood and cell samples lays the foundation for the potential development of a future blood biomarker assay for MNDs, providing new avenues for future research and diagnostic/therapeutic …
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Delineating the Steps of BAX Pore Activation
… suppressing oligomerization and pore formation. Oxysterols and bile acids are physiological derivatives of cholesterol. Further employment of our vesicle leakage regime revealed that 25-hydroxycholesterol at low micromolar concentrations accelerates BAX pore formation, suggesting a compensatory …
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Role of Macrophage Apoptosis in Atherosclerosis.
<p>The presence of apoptotic cells in atherosclerotic lesions has been broadly reported in the past ten years. The majority of these apoptotic cells are macrophages. However, the pathogenic role of macrophage apoptosis in the development of atherosclerosis remains to be elucidated. Elevated …
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Recettori purinergici ionotropi e i loro ruoli nella neuroinfiammazione mediata dalla microglia
La neuroinfiammazione è il più comune fenomeno coinvolto nella progressione delle malattie neurodegenerative, principalmente mediate dalla microglia. È noto che i recettori purinergici sono coinvolti in tali condizioni, osservazioni supportate anche dall'aumento dei livelli extracellulari di …