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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 37 for “"Oxidoreductases"”.
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Oxidoreductases and halogenated subtrates: Biocatalytic and crystallographic studies
En 2007 se implementó una regulación dentro de la Unión Europea conocida como ¿REACH¿ (Registro, Evaluación, Autorización y Restricción de Sustancias Químicas), cuyo objetivo es proveer una protección máxima a la salud humana y al medioambiente frente a los riesgos asociados con el manejo de los …
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Expanding the Role of Oxidoreductases in Benzylisoquinoline Alkaloid Metabolism in Opium Poppy
… steps in morphine biosynthesis are catalyzed by oxidoreductases belonging to the 2-oxoglutarate/Fe(II)-dependent dioxygenase gene family, namely thebaine 6-O-demethylase (T6ODM) and codeine O-demethylase (CODM). When assayed with a wider range of BIAs, CODM, T6ODM, and the functionally unassigned …
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Biophysical and structural studies of novel F420-dependent oxidoreductases in Mycobacterium tuberculosis
The Mycobacterium tuberculosis (Mtb) genome encodes hundreds of proteins with unknown or putative function based on sequence similarity to characterised proteins. Incomplete knowledge of Mtb functional genomics undermines efforts to understand the complex biological repertoire of this deadly human …
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Stereoselectivity, kinetics, and engineering of flavin/deazaflavin oxidoreductases for use in industrial biocatalysis
Biocatalytic approaches are widely recognized as valuable tools for producing pharmaceuticals and fine chemicals with high chemical and optical purity. A range of industrial syntheses that require complex structures and/or a high degree of stereoselectivity, which are often technically challenging …
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Stereoselectivity, kinetics, and engineering of flavin/deazaflavin oxidoreductases for use in industrial biocatalysis
Biocatalytic approaches are widely recognized as valuable tools for producing pharmaceuticals and fine chemicals with high chemical and optical purity. A range of industrial syntheses that require complex structures and/or a high degree of stereoselectivity, which are often technically challenging …
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Disulfide Bond Formation: Identifying Roles of PDI Family Thiol Oxidoreductases and ER Oxidant Pathways
Protein disulfide isomerases (PDIs) catalyze the oxidation and isomerization of disulfide bonds in proteins passing through the endoplasmic reticulum (ER). Although as many as 20 enzymes are classified as PDI family members, their relative contributions to protein folding have remained an open …
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Molecular Studies of the NADH:ubiquinone Oxidoreductases and Thebo-Type Terminal Oxidase of the Aerobic Respiratory Chain of Escherichia Coli
… cells. Three such proteins, two NADH:ubiquinone oxidoreductases and a terminal ubiquinol oxidase, from the respiratory chain of the bacterium Escherichia coli are addressed in this thesis. These three enzymes were examined by a variety of genetic and spectroscopic techniques.
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The Physical Chemistry Underlying the Assembly and Midpoint Potential Control in a Series of Designed Protein-Maquettes
… superoxide production (NADH oxidase). In natural oxidoreductases, determination of the course and rates of heme-protein association, what barriers are encountered, what affinity is achieved, and what are the oxidation-reduction potentials, is critical for understanding the rules of assembly and …
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Production and characterization of two AA3 enzymes from Arabidopsis thaliana
… class, the AA3 family, contains FAD-dependent oxidoreductases with diverse catalytic roles in fungi, where they participate in lignocellulose degradation. However, the biological functions of their plant counterparts remain largely unknown, and only a few plant AA3 enzymes have been …
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Preparation of a self-contained NADH co-factor recycling particle system
Oxidoreductases are interesting enzymes with potential applications in a number of different industries such as the textile, food and feed, chemical and biomedical industries. Oxidoreductases require the use of co-factors. These small molecules are relatively expensive and are required in …
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Comparison of Redox-Active and Conjugative Enzymes With and Without Efflux Transporters and Their Comparative Effectiveness in Protection Against Cellular Toxicity of 4-Hydroxy-2-Nonenal (HNE), an Aldehyde Lipid Peroxidation Production
… to have cytotoxic and genotoxic effects. Several oxidoreductases (ORs) have been reported to detoxify HNE including aldehyde dehydrogenase (ALDH) and aldo-keto reductases (AKRs). Glutathione S-transferases (GSTs) are known to play a role in the detoxification of HNE by the formation of an …
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Meccanismo catalitico della perossidasi da Euphorbia characias
Peroxidases (EC 1.11.1.1-16) are a group of oxidoreductases utilizing hydrogen peroxide, or other peroxides, to oxidize a second reducing substrate belonging to a large array of organic and inorganic compounds. A class III peroxidase isolated from the latex of Mediterranean shrub Euphorbia …
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Understanding the Effects of Higher-Order Assembly on the Structure of Sulfite Reductase
… multistep enzymatic reactions. Multi-subunit oxidoreductases can show inter- and intra-subunit domain interactions by switching from open to closed conformations in order to deliver electrons to their oxidase partners. Assimilatory sulfite reductase (SiR) is an NADPH-dependent multidomain …
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Characterizing Factors That Influence Intracellular Thiol-Disulfide Equilibrium
… proteins). This project focuses on how specific oxidoreductases and their assembly factors influence the subcellular GSH:GSSG redox potential. In one study, deletion of the mitochondrial iron homeostasis factor Mtm1 was found to cause a large oxidative shift in the GSH:GSSG redox potential in …
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Strategies to enhance extracellular electron transfer rates in wild-type cyanobacterium Synechococcus elongatus PCC7942 for photo-bioelectricity generation
… mechanism were overexpressed (2-fold). However, oxidoreductases in the outer membrane remain to be identified. Furthermore, electroactive regions in biofilms of S. elongatus PCC7942 were established using cyclic voltammetry. Double step potential chronoamperometry was also successfully tested in …
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Controlling Bacterial Persister Cells and Biofilms by Synthetic Brominated Furanones
… gene mdaB in both strains and 7 genes encoding oxidoreductases in P. aeruginosa. In E. coli RP437 persister cells, BF8 was also found to repress the genes for synthesizing indole, a signaling molecule reported to induce persister formation in E. coli. Interestingly, although BF8 is a QS …
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Developing assays to study and screen engineered Escherichia coli NADPH-dependent sulfite reductase
… that participate in these reactions (e.g., oxidoreductases) through protein engineering. However, successful enzyme engineering studies depend on appropriate assays to evaluate and characterize the desired variants. The Escherichia coli NADPH-dependent sulfite reductase is a …
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The effect of dissolved oxygen on recombinant protein degradation in Escherichia coli
… was influenced by elimination of cytoplasmic oxidoreductases that increased the redox potential of the cytoplasm, perhaps indicating an interaction between cellular redox status and the observed degradation and yield of a 1AT. Low molecular weight fragments of [alpha]1AT were eliminated from …
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Uncovering Biophysical Determinants Of Oxidoreductase Function Through De Novo Protein Design
Oxidoreductases are a diverse class of enzymes defined by their chemical role of transferring electrons from one substrate to another. Comprising the functional cores of photosynthetic, chemosynthetic, and cellular respiratory machinery, “redox proteins” and the catalysis they enable form the …
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