Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 14 of 14 for “"Oxidative decarboxylation"”.
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Studies on the Mechanism of the Pyruvate Oxidase Flavoprotein of Escherichia Coli (Rapid Kinetics, Biochemical Software, Lipid-Activated Enzyme)
The pyruvate oxidase flavoprotein catalyzes the oxidative decarboxylation of pyruvate to acetate and carbon dioxide. Flavin adenine dinucleotide and thiamin pyrophosphate are cofactors. The enzymatic activity of the purfied flavoprotein is very low in the absence of lipids. Following reduction by …
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Hydrogen Peroxide Inhibition and Inactivation of Bicupin Oxalate Oxidase
… proposals that the enzyme catalyzed the non-oxidative decarboxylation of this three carbon substrate. Finally, we observe a <sup>13</sup>Ckinetic isotope effect on the CsOxOx catalyzed reaction using MIMS which supports that the carbon-carbon bond cleavage of oxalate is at least partially …
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Molecular Analyses of the Soybean Glabrous Mutation (P1) and of Transgenic Lines Designed to Modify Seed Composition
… subunit of the PDC. The PDC catalyzes the oxidative decarboxylation of pyruvate into acetyl-CoA and thereby controls the entrance of carbon subunits into the TCA cycle. The activity of the PDHK protein is enhanced during periods of active photosynthesis, which leads to downregulation of the …
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Quinone-Catalyzed Amine α-C–H and α-C–C Functionalization
… The excellent reactivity of quinone in oxidative amine C–H functionalization motived to us to investigate quinone-catalyzed oxidative C–C functionalization of amine-containing molecules. In this regard, we developed a novel method for homologation of aromatic α-amino acids through …
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Microbial Degradation of Acetaldehyde in Freshwater, Estuarine and Marine Environments
… chemistry by altering the atmosphere’s oxidative status or “self-cleaning capacity”. Acetaldehyde flux from freshwater, estuarine, and marine environments is thought to be under significant microbial control, yet the identity and diversity of microbial acetaldehyde degraders, and the …
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Mechanistic studies on chorismate mutase-prephenate dehydrogenase from E. coli
… to (4-hydroxyphenyl)pyruvate through an oxidative decarboxylation reaction catalyzed by prephenate dehydrogenase. Through chemical modification and site-directed mutagenesis we have identified residues that are critical in both the mutase and dehydrogenase mechanisms and have provided …
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Mechanistic Studies of Flavin-Dependent Monooxygenases Involved in Bacterial Defense and Plant Metabolism
… S-oxygenation, Baeyer-Villiger oxidation, and decarboxylation, using NAD(P)H as a coenzyme. In this dissertation, the characterization of several FMOs from bacteria and plants is described. The putrescine N-monooxygenase (NMO) FbsI from Acinetobacter baumannii is involved in the fimsbactin …
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New Insights into Mycofactocin Biosynthesis, Structure and Function
… enzyme MftC, catalyzes the SAM-dependent oxidative decarboxylation and carbon-carbon bond formation on MftA to form MftA*. The roles of the auxiliary [Fe-S] clusters in MftC catalysis as well as the subsequent steps in the biosynthesis of mycofactocin are not known. Here, we have provided …
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Mass Spectrometric Analysis of Tyrosine Metabolic Enzymes
… components revealed that AMD catalyzes the oxidative decarboxylation of L-DOPA to DOPAL, and also the oxidative decarboxlation of α-methyldopa to 3,4-dihydroxyphenylacetone. In summary, multiple N-glycosylation sites were characterized in DCT and DCE, furthermore a new protein function has …
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Preclinical Studies of A Novel Idh1 Inhibitor In Acute Myeloid Leukemia (Aml)
… IDH1 is a cytoplasmic enzyme that catalyze the oxidative decarboxylation of isocitrate to α-ketoglutarate (α-KG) in the citric acid cycle. Somatic gain-of-function mutations in IDH1 occur in ~10% of the newly diagnosed AML patients. The neo-enzymatic activity of mutant IDH1 results in …
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Three enzymes - One substrate Regulation of carbon flux through a "non-canonical" metabolic branchpoint
… However, many bacteria are able to bypass these oxidative decarboxylation steps, allowing them to conserve carbon for gluconeogenesis. They perform this by using the “glyoxylate shunt”. Here, isocitrate is cleaved by isocitrate lyase (ICL) to yield succinate and glyoxylate (which, in a subsequent …
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Study of PVCA and PVCB, two enzymes involved in making isonitrile-containing natural products in bacterial pathogens
… demonstrated that XnPvcB catalyzed an oxidative decarboxylation reaction on tyrosine isonitrile, while the PaPvcB and EaPvcB reactions retained the carboxyl group. Steady-state and transient-state kinetics were performed to determine the catalytic efficiency and the mechanisms of the …
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Immunological and biosynthetic studies of the human pyruvate dehydrogenase complex
… multi-enzyme complex (PDC) catalyses the oxidative decarboxylation of pyruvate, transferring the resultant acetyl group to coenzyme A. It belongs to the family of 2-oxoacid dehydrogenase complexes that includes the 2-oxoglutarate dehydrogenase (OGDC) and branched-chain 2-oxoacid …