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Showing 1 to 20 of 43 for “"Ouabain"”.
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In vitro metabolism of atrial and ventricular myocardium: effect of ouabain
This document only includes an excerpt of the corresponding thesis or dissertation. To request a digital scan of the full text, please contact the Ruth Lilly Medical Library's Interlibrary Loan Department (rlmlill@iu.edu).
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Studies on preconditioning with adenosine, glutamate and ouabain in rat hippocampal slices
… of the sodium-potassium ATPase inhibitor, ouabain, upon the evoked responses were studied as an alternative insult. It was shown that ouabain induced depressions in field epsps, orthodromic and antidromic population spikes. The antidromic population spikes showed significantly smaller …
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Na,K-ATPase signaling in cyst progression in autosomal dominant polycystic kidney disease
… to increase ADPKD progression is the hormone ouabain. Ouabain is a cardiotonic steroid (CTS) that circulates normally within mammals. It and other CTS compounds are obtained through the diet and produced as endogenous hormones, with normal functions including cell proliferation and …
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ROLE OF THE Na,K-ATPase IN POLYCYSTIC KIDNEY DISEASE
… of the Na,K-ATPase is regulated by the hormone ouabain, which inhibits its movement of the cations. In addition, ouabain binding to the Na,K-ATPase has been been found to activate a cascade of phosphorylating events, leading to cell growth. Interestingly, we have found a population of the …
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Potassium and Sodium Movements Related to Growth State and Malignant Transformation in Cultured Chicken Embryo Cells
… potassium transport and the associated specific ouabain binding were shown to be dependent on the cellular growth state but unaffected by malignant transformation; potassium influx and ouabain binding values in growing normal and transformed chicken embryo fibroblasts were 1.5 to 1.8-fold greater …
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The Effects of total and partial inhibition of the sodium and pottassium-activated Adenosine Triphosphatase on the isolated perfused rabbit heart
… mmol. 1“’' or zero, or by including luraol.l ’ ouabain in the control medium (K<, = 4.9 mmol. 1 ’). A reduced Kg or ouabain produced a progressive net cellular K loss,net cellular gains in Na and Ca,and qualitatively similar declines in ATP and CP.These studies indicated primary roles for Ca …
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Organismi acquatici e ambiente: meccanismi biochimici di interazione, risposta e adattamento
… and seawater pHs, with the Na,K-ATPase and the ouabaininsensitive Na-ATPase was investigated in vitro on gill and mantle microsomal membranes. The proven replacement by NH4 +of K+ in the activation of the Na,KATPase and of Na+ in the activation of the ouabain-insensitive ATPase displayed similar …
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Sustained Depolarization of the Resting Membrane Potential Regulates Muscle Progenitor Cell Growth and Maintains Stem Cell Identity in vitro
… gluconate or the sodium-potassium pump blocker ouabain and evaluated for proliferation, DNA content using propidum iodide staining, and expression of myogenic markers. Cell proliferation measurements showed a stimulatory effect with lower concentrations of both agents, but higher concentrations …
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Characterization, purification, and DNA sequence analysis of a novel ATPase of the archaebacterium Methanococcus voltae
… bafilomycin A$\sb1$, nitrate, ouabain and oligomycin. The enzyme displayed a high preference for ATP as substrate, was dependent on Mg$\sp{2+}$, and had a pH optimum of 7.5. The enzyme was completely solubilized with 2% Triton X-100. It was insensitive to oxygen and was …
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Regulation of Intracellular pH and Plasma Membrane Potential in Trypanosoma Cruzi and Trypanosoma Brucei
… significant contribution to its DeltaPsi from a ouabain sensitive Na+/K+-ATPase. The epimastigote or insect stage was found to have a low conductance for cations and a minor contribution from the Na+/K+-ATPase. There was no evidence for cation conductance or a Na+/K+-ATPase in the intracellular …
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Activity of Sodium Ion, Potassium Ion in the Epididymis of the Rat
… was potassium-dependent and was inhibited by ouabain.
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The effects of prolonged exercise on Na+, K+ - ATPase and SR Ca2+ regulation in humans
… 3-0-MFPase activity), Na+K+ATPase content (3[H] ouabain binding sites), SR Ca2+ release rate induced by 4 chloro-m-cresol and Ca2+ uptake rate.
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Higher Brain Neurons Succumb to Acute Stroke-Like Insult While Lower Brain Neurons Strongly Resist
… injury. Exposure to the Na+/K+ ATPase inhibitor ouabain (100 μM), induces depolarization similar to OGD in all cell types tested. Moreover, elevated [K+]o evokes spreading depression (SD), a milder version of AD, in higher brain but not hypothalamus or brainstem so weak AD correlates with the …
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Intracellular calcium mobilization in cardiomyocytes of rats with experimental diabetes
… maximum response but an increased sensitivity to ouabain stimulation. This suggests that diabetic cells are less tolerant to ouabain, which may be related to the depressed Na-pump activity and increased [Na]i in diabetic hearts. ATP and KCI both caused an enhanced increase in [Ca2]i in diabetic …
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Physiological Role of the α<sub>2</sub>-Isoform of the Na, K-ATPase in the Regulation of Cardiovascular Function
… in an increase in the level of endogenous brain-ouabain, resulting in hypertension that is dependent on the ouabain-binding site of the α<sub>2</sub>-isoform in the brain. In addition, several reports have shown that an increased level of endogenous brain-ouabain activates the renin-angiotensin …
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Sodium and glucose transport across the in vitro perfused midgut of the blue crab, Callinectes sapidus Rathbun
… The flux displayed saturation kinetics and was ouabain-sensitive. The flux did not vary with environmental salinity, thus providing no evidence for an active regulatory role of the midgut in sodium balance. A reduction of passive permeability with salinity, however, was suggested. There was no …
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Physiological, Biochemical, and Molecular Biological Effects of +4⁰C Acclimation on the Antarctic Teleost Trematomus bernacchii
… dependent activation of the Na+,K+- ATPase, and ouabain inhibition of phosphoenzyme intermediate formation. Acclimation to +4°C caused the T. bemacchii to significantly decrease its serum osmolality from 581 + 11 mOsm kg"1 at -1.5°C to 477 + 4 mOsm kg'1 at +4°C. The apparent affinity of the …
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The Na/K-ATPase in the gills of Antarctic and New Zealand nototheniids: The Physiologic and Molecular Effects of Warm-acclimation.
… Na/K-ATPase activity, sodium pump density and ouabain affinity. Both fishes were acclimated at their upper and lower viable thermal temperatures. Warm acclimation (+4°C) of the 7. bernacchii significantly decreased their serum osmolality from 550 mOsm/kg to 450 mOsm/kg compared to …
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Comparison of Ecto-Nucleotidase Activity in PAR-C5 Cells and Native Rat Parotid Gland
… Ca** and Mg** and resistant to the inhibitors, ouabain and vanadate. The enzyme was non-selective as to nucleotide substrate. Assays in the presence of 100 pM of each of the substrates gave the following relative activities (%) compared to that of 100 uM ATP; GTP (104.5±7.9), ITP (87.4±9.4), UTP …
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