Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 37 for “"Osteoblast Differentiation"”.
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The Regulation of Glucose Metabolism during Osteoblast Differentiation
<p>Differentiation and cell-specific functions are coupled with metabolic alterations to meet the needs of the cell. In this thesis, I have investigated the alterations of cellular metabolism in osteoblast-lineage cells in response to two different bone anabolic signals, WNT and PTH. I have further …
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The Hematopoietic Compartment Regulates Osteoblast Differentiation And Apoptosis During Cytokine Treatment
… signaling, we unexpectedly uncovered a role for osteoblasts in regulating cytokine-induced mobilization. By isolating and sorting different fractions of bone marrow stromal cells we demonstrate that osteoblasts represent a major source of CXCL12 in the bone marrow. In addition, both the number …
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Apoptosis and Osteoblast Differentiation in the Periodontal Lignament in and in vivo Orthodontic Relapse Mouse Model
… Proper characterizations and quantification of osteoblast differentiation in a mouse model of orthodontic relapse have yet to be demonstrated. It is the goal of this study to characterize and quantify osteoblast differentiation in orthodontic relapse in an <em>in vivo</em> transgenic mouse …
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Modulation of Osteoblast Differentiation in Rat Calvarial Cells: Investigations into Effects of Mechanical Load and Interleukin-1β
… if this represents upregulation of existing osteoblasts or increased differentiation of osteoblasts. In vitro effects of mechanical loading of osteoblast-like cells include increased proliferation, changes in collagen production and increased prostaglandin formation. No in vitro study has …
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Roles for C-ABL and P53 in bone homeostasis and DNA damage response
… bone resorption. p53 negatively regulates osteoblast differentiation and osteoblast-dependent osteoclastogenesis, via repressing the transcription of osterix, an essential osteoblast differentiation factor. Inactivating p53 rescues osteoblast differentiation defects of mice lacking c-Abl, a …
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조골세포 사멸 및 분화에서 GSK3b의 역할
" Dfferentiation of osteoblasts is critically involved in bone formation and requires several signal pathways. One of the important pathways is the Wnt signaling where GSK3 plays a key role in regulation of β-catenin activity, a major effector, through phosphorylation. Glucocorticoids (GCs) are …
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Interactions between BMP and Canonical Wnt Signaling Regulate Critical Stages of the Osteoblast Lifecycle
… activity of bone-forming cells called osteoblasts and bone-resorbing cells called osteoclasts. Over 10 million people in the US currently suffer from osteoporosis, which increases the risk of low-trauma fractures and accounts for health care expenditures exceeding $14 billion per year. …
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Aberrant Ras/MAPK signaling in skeletal development
… bone mass is regulated via K-ras/MAPK control of osteoblast differentiation we have generated two novel mouse models. Specifically, to investigate how hyperactive K-ras affects skeletal development when expressed in a spatial and temporal specific manner we activated K-rasG12D at various stages …
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Factors affecting bone cell growth and differentiation under differing culture conditions
Marrow stromal cell (MSC) differentiation into osteoblasts is an important part of the bone growth and remodeling process. This process can be exploited to help solve the problem of bone wound healing. Because of problems with bone grafts, implantation of biodegradable 3D scaffolds seeded with MSCs …
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The Molecular Mechanisms of Estrogen Receptor α on Two Single Nucleotide Polymorphisms to Regulate WNT Signaling in Osteoblasts
… of these SNPs has remained unknown. Analysis of osteoblasts from 112 different joint replacement patients reveals that the genotype of rs2887571 correlates with WNT5B expression, and the genotype of rs9921222 correlates with AXIN1 expression. Mechanistically, SNP rs2887571 has less binding of ERα …
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A Mechanistic Evaluation of how Melatonin Enhances Alkaline Phosphatase Activity in Human Adult Mesenchymal Stem Cells
… osteoclasts. Decreased bone producing osteoblast efficiency however, is largely responsible for osteoporosis development. Therefore, novel osteoporosis treatments should stimulate osteoblast formation and activity. Accordingly, the purpose of this study was to determine melatonin's role …
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Non-autonomous regulation of bone mass accrual and the role of T-cell protein tyrosine phosphatase in the bone regulation of insulin sensitivity
… both the cell-nonautonomous regulation of osteoblast differentiation and the bone regulation of energy metabolism using cell-specific gene inactivation in the mouse. Early B-cell factor 1 (Ebf1) is a transcription factor whose inactivation in all cells results in high bone mass because of …
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Nmp4 restricts bone marrow osteoprogenitors and parathyroid hormone induced bone formation in healthy and estrogen depleted female mice
… osteoprogenitors and CD8+ T-cells which support osteoblast differentiation. The animals were not protected from bone loss following OVX, but retained the hypersensitivity seen in the intact mice. Mesenchymal stem/progenitor cells (osteoprogenitors) demonstrated increased growth rate in culture …
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The Fruit of Poncirius Trifoliata Promotes Mesenchymal Stem Cell Differentiation into Osteoblasts in Glucocorticoid-induced Osteoporosis Model
… of poncirus trifoliata on the activities of osteoblast and animal model. Effects of osteoblastic differentiation were measured by alkaline phosphatase (ALP) activity, osteopontin (OPN) protein expression and mineral nodule formation in dexamethasone (DEX)-treated MC3T3-E1 cells and primary …
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Novel Insights into CB1 Receptor Signaling and the Anabolic Role of Cannabinoid Receptors in Bone
… different diseases. The cannabinoid receptors of osteoblasts may represent a target for the treatment of bone disorders such as osteoporosis. Our research demonstrates that cannabinoids can affect important signaling molecules in osteoblasts. In MC3T3-E1 osteoblastic cells, the CB1 antagonist, …
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Molecular Mechanism of the Stimulation of Alkaline Phosphatase Activity in Human Bone Cells by 1,25(OH)2 D3
… that exhibited many aspects of normal osteoblasts. The human osteosarcoma cell line (TE85 cells) expressed a skeletal alkaline phosphatase activity (an accepted bone cell differentiation marker) which was stimulated by 1,25(OH)<sub>2</sub>D<sub>3</sub> (a potent differentiating agent), …
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A Study of End-functionalized Polylactide based Thin Films for Biomedical Applications
… of PLA by ionic groups in ionomeric PLA enhanced osteoblast differentiation compared to the traditional PLA based materials; however, formation of mineral nodules was suppressed, possibly due to the ions. Since topography did not influence cell behavior in the first set of studies, influence of a …
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Regulation of IGFBP-5 and Osteoblast Functions by Nuclear Factor I
… (IGFBP5) which binds IGFs and stimulates osteoblast activity by IGF dependent and independent mechanisms. GC-induced inhibition of IGFPB5 promoter activity was mediated by a composite response element that has binding sites for transcription factor activator protein 2 (AP-2) and nuclear …
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Novel Molecules that Regulate Bone Formation and Bone Resorption
… resorption. We demonstrated that Grx5 protects osteoblasts from oxidative stress induced apoptosis via a molecular pathway that involves regulation of reactive oxygen species production, cardiolipin oxidation, caspase activity, iron-sulfur cluster formation, and manganese superoxide dismutase …
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Bmp-Signaling Regulates A Common Transcriptional Program to Control Facial Form and Skeletal Morphogenesis
… regulators controlling self-renewal, osteoblast differentiation, and negative Bmp autoregulation. The complimentary experiment, CNC inactivation of <em>Bmp2</em>, <em>Bmp4</em>, and <em>Bmp7</em>, resulted in complete or partial loss of multiple CNC derived skeletal elements revealing …
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