Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 27 for “"Osmotic Shock"”.
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The effect of osmotic shock on the release of acid phoshatase activity from streptococcus mutants
The "osmotic shock" treatment of bacterial cells has proven to be an effective procedure for releasing hydrolytic enzymes located in the periplasmic compartment of the cell. It was the object of the present study to examine the mechanism of the "osmotic shock" procedure on the Streptococcus mutans …
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Copper/zinc Sods of Salmonella Typhimurium: Enzymatic Properties and Correlation to Pathogenesis
… dimer and is not released from the periplasm by osmotic shock. Thus SodCI is ""tethered"" in the periplasm by some non-covalent interaction. In contrast, SodCII is monomeric and is quantitatively released by osmotic shock. Using site-directed mutagenesis we constructed a monomeric, fully active …
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Antimicrobial Effectors Act Cooperatively to Stress Salmonella in the Macrophage Phagosome
… is protease sensitive, and is released by osmotic shock, like other known periplasmic proteins. In contrast, SodCI forms dimers, is protease resistant, and is retained within the periplasm after osmotic shock, a phenomenon that we term tethering. We are further characterizing SodCI …
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Development of a Controller for Fermentation in the Dry Grind Corn Process
… Reduced glucose concentrations minimize the osmotic shock to yeast and inhibit the growth of other competing microorganisms. Use of the optimal controller resulted in 50% reduction in glucoamylase amount required for the SSF process under varying operating conditions as compared to the …
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Improving the recovery of "difficult to release" periplasmically-expressed products from recombinant E. Coli
… expressed proteins at large-scale. The classical osmotic shock procedure, the only reliable method for releasing proteins from the periplasmic space of E. coli, is expensive and time-consuming, and thus it is not feasible at large scale. The main objective of this study has been to develop a …
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A Major Cell Envelope Protein Of Escherichia Coli K12: Purification And Properties
… reported. SEP was released by EDTA-sucrose and osmotic shock treatments, or during the formation of spheroplasts. The protein was purified on columns of aspartate-Sepharose (A-S) an affinity matrix previously used for the isolation of dicarboxylate transport components. SEP was the major protein …
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Regulation of hnRNP A1 Cellular Localization by Protein Kinases and its Biological Impact
… cytoplasmic accumulation of hnRNPA1 during osmotic shock and HRV infection, to a decrease in HRV-infected cells and to decreased caspase activation in osmotically stressed and HRV-infected cells. Thus, HK2 may regulate hnRNP A1 cytoplasmic localization following HRV infection.
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Pollen digestion in flower-feeding Scarabaeidae : protea beetles (Cetoniini) and monkey beetles (Hopliini)
… rodents, marsupials, bats and birds) may use osmotic shock, pseudo-germination, exudation, microbial digestion or enzyme penetration to gain access to the protoplasmic contents. Further study is needed to define the details of these methods and whether they are all in use.
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The role of the Dgk1 and Pah1 in lipid interconversion on saccharomyces cerevisiae vacuole physiology, homeostasis, protein sorting and fusion
… though these vacuoles were able to reform after osmotic shock, similar to the wild type background, but unlike either single deletion strain. This strain displayed reduced fusion and, interestingly, what appeared to be either the aberrant production, or degradation, of proteins. This observation …
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Chitin Synthesis -Related Proteins in the Chitin -Less Yeast Schizosaccharomyces Pombe
… of SpChs1p resulted in an increased tolerance to osmotic shock, and loss of SpChs2p resulted in an increased tolerance to heat-shock. Both the deletion or the overproduction of SpChs4p and SpChs5p led to phenotypes. Deletion of Spchs4 + resulted in cells with a cell separation defect, and these …
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Dynamics of gene expression and signal transduction in single cells
… protein kinase (MAPK) cascade to osmotic shock, combining experimental measurements and theoretical models.
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Respuestas fúngicas a señales ambientales: Funciones de las proteínas CarO, OpsA, CutA y AcyA en Fusarium fujikuroi
… on their ability to adapt to variations in osmotic conditions. In Chapter 2, we provide novel data on the molecular mechanism used by F. fujikuroi to overcome hyperosmotic stress. The research group formerly described the gene cut-1 of Neurospora crassa, encoding a protein of the haloacid …
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Investigations into mechanisms of magnesium homeostasis in the isolated rat heart
… chloride, specific ion channel inhibitors, osmotic shock, metabolic inhibition and global ischaemia, in the isolated, perfused (5 ml mm", 37°C) and paced (20V, I msec, 4-6 Hz) rat-heart were investigated in an attempt to characterize further, the mechanism of Mg 24 transport. Following a …
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Characterization of the Broad-Spectrum Racemase, BsrF, in Vibrio fischeri
… as D-Met, did not convey protection against an osmotic shock or provide resistance to vancomycin or D-cycloserine, two antibiotics that target peptidoglycan metabolism. However, I found that the catabolism of D-Arg, and specifically its use as a nitrogen source, is dependent on BsrF. I also …
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Characterization of The Agrobacterium Tumefaciens Virb2 Pilin of The Virb/D4 Type Iv Secretion System
… further supported by coimmunoprecipitation and osmotic shock studies. Mutational studies identified two regions of VirB10, an N-terminal transmembrane domain and an outer membrane-associated domain termed the antennae projection, that contribute selectively to T-pilus biogenesis. Lastly, …
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Life Under Tension: Computational Studies of Proteins Involved in Mechanotransduction
… been proposed to act as a safety valve during osmotic shock, facilitating the release of ions and small solutes upon increase in membrane tension, thereby preventing bacterial cells from bursting. The second set of proteins studied are ankyrin and cadherin repeats, likely forming part of the …
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A study of the decay of acid cationic ion exchange resin
… during the loading phase. The phenomenon of osmotic shock in addition to resin cracking and swelling is clearly observed. A further qualitative study bore out the change in resin rigidity as the resin is artificially degraded through repeated loadings and regenerations performed by using a …
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Robustness and tunability in biological networks/ by Shankar Mukherji.
… modules: a phosphorelay module that senses the osmotic shock signal that feeds into a mitogen-activated protein kinase (MAPK) module. Using a combination of systematic complementation experiments and computational sensitivity analysis, we show that the phosphorelay module is robust to changes in …
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A biochemical and physiological characterization of coenzyme F420-reducing hydrogenase from Methanobacterium formicicum
… Neither enzyme was released from whole cells by osmotic shock treatment.
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Identification of substrate proteins of FtsH during sporulation of Bacillus subtilis
… as filamentous growth, sensitivity towards heat, osmotic shock and cells are unable to sporulate. Recently, it has been shown that ftsH knockout cells fail to entry sporulation stage II due to lack of a sufficient amount of Spo0A~P and the first substrate of FtsH identified in B. subtilis is the …
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