Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 30 for “"Oryzias latipes"”.
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A genetic analysis of somitogenesis in the Medaka (Oryzias latipes)
… a large scale mutagenesis screen in the medaka (Oryzias latipes) has been performed successfully in Kyoto, Japan. I analyzed nine of the isolated medaka mutants that show variable aspects of somitic phenotypes. This includes a complete or partial loss of somite boundaries (e.g. bms and sne), …
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Novel Approaches to Transgenesis in the Teleost Medaka (Oryzias latipes)
The aim of thesis was to improve the generation of transgenic medaka fish. General transgenesis including transient expression of reporter genes and germ line integration of reporter genes was improved by application of two novel techniques. In addition, one of these methods allows for the first …
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Valproic Acid-Induced Teratogenesis in Japanese Rice Fish (Oryzias Latipes) Embryogenesis
… We are developing the Japanese rice fish (Oryzias latipes), also known as Japanese medaka, as an animal model to study valproate-induced teratogenesis during the period of embryogenesis. Fertilized embryos of Japanese rice fish at three developmental stages (group A: 0–2 dpf; group B: 1–3 …
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Hypoxic Stress Induced Gene Modulation in Medaka (Oryzias latipes) and Xiphophorus maculatus
… in four tissues (brain, liver, gill, and fin) of Oryzias latipes and Xiphophorus maculatus. We analyzed six potential gene targets that had previously been shown in microarray studies to exhibit robust (> 5.0 fold) changes in mRNA abundance when medaka were exposed hypoxic conditions. Herein we …
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The Influence of Estrogen Signaling on Male Reproduction in Medaka (Oryzias latipes)
<p>Endocrine disrupting chemicals (EDCs) are ubiquitous and often act as xenoestrogens with the ability to disrupt estrogen signaling through differential binding to the various estrogen receptors. Exposure to these xenoestrogens has led to detrimental effects on male reproduction. In fish, …
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Examining swim bladder inflation failure as a toxicological endpoint in Japanese medaka (Oryzias latipes)
… swim bladder inflation of Japanese medaka (Oryzias latipes). After exposure to individual compounds, mixture exposures to no-observed-effect concentrations of these compounds were conducted. Significant effects on swim bladder inflation were observed for a binary mixture of …
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The Effects of Toluene on the Ontogenetic Development of the Fish, Oryzias latipes, the Japanese Medaka
… development of the Cyprinodontid fish, Oryzias latipes, the Japanese Medaka was established. By the use of static bioassays, the median tolerance limits (TL 50 values) were calculated with their respective 95% confidence limits for different stages of embryonic development exposed to the …
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Organ and primary culture of medaka (Oryzias latipes) testis: Test systems for the analysis of cell proliferation and differentiation
In cultured medaka testis fragments, cells remained viable for the entire culture period (17h), and spermatids that developed from spermatocytes were viable and motile. Primary cultures were characterized over a period of two days with respect to cell viability and the distribution of adherent and …
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Standardizing and Optimizing Rearing Methods of Animal Models: Influence of Diet on Growth and Reproduction in Xiphophorus Maculatus and Oryzias latipes
… models, Xiphophorus maculatus (platy fish) and Oryzias latipes (medaka), were used to evaluate dietary effects on growth and reproductive performance. Platy fish (n =120) and medaka (n =300) were reared from birth to one month of life on Ziegler Aquatox flakes and live Artemia nauplii, then …
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Aggression, monopolization and growth depensation within groups of Japanese medaka (Oryzias latipes) : interactions between the temporal and spatial clumping of food
… food by allowing groups of ten Japanese medaka (Oryzias latipes) to compete, over a 4 week period, for food in a 2 x 2 factorial design. As expected, the intensity of aggression increased as the spatial clumping of food increased and as the temporal clumping of food decreased. However, there was …
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Silence of the fish: injection of photoswitchable short interfering RNA oligonucleotides into Japanese medaka embryos (oryzias latipes) to photochemically control gene silencing
The siRNA duplex functions by binding to and cleaving mRNA, a process known as the RNAi pathway. Existing siRNAs face challenges such as off-target effects and unpredictable prolonged gene-silencing. To address these issues, enhancing their therapeutic potential, siRNAs can be modified controlling …
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Chemical, Toxicological, and Microbial Characterization of New Orleans Sediments Following Hurricane Katrina
… and toxicity was tested using Japanese medaka (Oryzias latipes) embryos. Sediments with the highest contaminant levels showed the highest embryonic mortality and most delayed development. However, no sediment caused an increased mutant frequency. When the most contaminated site was resampled in …
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Cloning of medaka glial cell-derived neurotrophic factor (GDNF) and its receptor GFR alpha 1
… both Gdnf and GfrI?1 from the medaka fish (Oryzias latipes). A full length cDNA for the medaka gfrI?1 was obtained by RT-PCR followed by RACE. It is 2551 bp long and has an open reading frame of 1401 bp for 466 amino acids. The predicted protein shares the best homology to the known …
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Population Genetics of Death Valley Pupfishes (Cyprinodontidae:Cyprinodon Spp.) and the Identification of a New Retrotransposable Element Family
… family was undertaken in the Japanese medaka (Oryzias latipes) as well as in the pupfish genome. The Japanese medaka is a model organism widely used for genetic and developmental biology studies.
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Various aspects of reproductive control in freshwater fishes
… administered orally to juvenile Japanese medaka (Oryzias latipes) in two experiments to determine the possible role of the antiandrogen on sex determination. Sex ratios were determined in the mature fish by dimorphic coloration, secondary sex characters, and histological examination. Sex reversal …
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Perspectives On Ethanol Toxicity In Medaka Embryogenesis And Possible Protection By Asian Ginseng (Panax Ginseng): Histological And Biochemical Analysis
… zebrafish (Danio rerio) and Japanese medaka ( Oryzias latipes), are long-established models for research in developmental biology and have been used to explore alcohol's effect on neurogenesis, cardiogenesis, intracellular signaling, neurobehavioral aspects, and apoptosis. These two fish models …
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Embryotoxicity of dioxin-like chemicals extracted from American eels (Anguilla rostrata) from the St. Lawrence River System
… offspring, as assessed by using Japanese medaka (Oryzias latipes), a surrogate species. Medaka embryos were first injected with 2,3,7,8-tetracholordibenzo-p-dioxin (TCDD) to establish their sensitivity to this test chemical expressed as an 11-day EC50 of 3.79 pg/mg, for the induction of BSD. …
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Study of the role of miR-204 in photoreceptor development
… and maturation by means of <i>in vivo</i> (<i>Oryzias latipes</i> i.e. medaka fish) and <i>in vitro</i> (661W cell line) systems. I found that, the gain-of-function of miR-204 led to an earlier exit of photoreceptor precursors from cell cycle and to a concomitant earlier ‘fate determination’, …
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Development and Standardization of a Short-Term Assay for Evaluating Polluted Estuarine and Coastal Environments: The Medaka Embryo-Larval Assay
<p>The eggs of the Japanese medaka (Oryzias latipes) were employed in a number of studies to develop a routine, standardized assay which can assess the acute and sublethal impacts of individual toxicants and complex mixtures. The eggs of this Cyprinodont minnow were topically treated with each …
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