Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 80 for “"Orthologues"”.
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The Evolution of Gap Gene Orthologues
Die Segmentierung in der Fliege Drosophila melanogaster ist weitgehend verstanden. Als sogenannter "Langkeimer" entwickelt sich die Fliege jedoch anders als die meisten anderen Insekten: Bei der Fliege entstehen alle Segmente auf einmal im Blastoderm. Im Mehlkäfer Tribolium castaneum und den …
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Analysis of the sltA (stzA) gene and its orthologues in Aspergillus nidulansand other filamentous fungi
… amino acid similarity. All twenty-nine StzA orthologues identified are restricted to filamentous ascomycetes of the Pezizomycotina subphylum and may therefore represent specific and novel antifungal drug targets. The C-termini of StzA proteins are highly variable in both length and sequence, …
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Probing Orthologue and Isoform Specific Inhibition of Kinases using In Silico Strategies: Perspectives for Improved Drug Design
… and kinase targeting domains, we found that orthologues and isoforms contain distinct features, likely responsible for their biological roles, which can be utilized and exploited for selective drug development. In this work, we identified new exploitable features between kinase orthologues …
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Cloning and characterisation of LEA1-EM genes in the resurrection plant, Xerophyta humilis
The presence and expressIon patterns of orthologues of LEA group 1 genes has been characterised in the resurrection plant, Xerophyta humilis. The group I LEAs (Em I and Em6) were first identified as proteins that were abundantly and specifically expressed during the desiccation and germination …
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INVESTIGATION OF THE DISTAL APPENDAGE PROTEIN CEP164 IN Trypanosoma brucei
… and surrounding structures. Three diverse Cep164 orthologues in the T. brucei parasite have been identified, but knowledge on their function is limited as functional analysis of these proteins has not been carried out. This project, in collaboration with the TrypTag project used a bioinformatic …
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Biochemical and molecular characterisation of cell wall glycosyltransferases in Mycobacterium tuberculosis
… M. tuberculosis and deleted their respective orthologues in C. glutamicum. Mutant phenotypes were characterised biochemically using, two-dimensional-thin layer chromatography, SDS-Polyacrylamide-gel electrophoresis, gas-chromatograpgy-mass spectrometry, nuclear magnetic resonance spectrometry …
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Alternative splicing in the regulation of the barley circadian clock
… identified, 60% of which are true Arabidopsis orthologues. Most of the barley clock genes have a clear daily rhythm which is maintained in constant light conditions. Mutations of the barley clock genes HvPPDH1 (orthologue of AtPRR7) and HvELF3 strongly affect flowering time and have extended …
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Comparative phylogenetic exploration of the human mitochondrial proteome: Insights into disease and metabolism
… a manually curated dataset of 190,097 predicted orthologues of the 1,550 IMPI 2017 human genes across 359 species, using reciprocal best hit analysis as the basis for orthologue prediction. I used this to explore gene history and the potential for phylogenetic profiling to predict the function of …
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Plastid to Nucleus Signalling and the Evolution of C4 Photosynthesis
… shown that in C3 Arabidopsis thaliana C4 orthologues can be controlled by plastid-to-nucleus signalling (Burgess et al. 2016). This strongly implies that evolution has re-enforced existing regulatory networks that operate in the C3 state to control expression of C4 genes. Currently, the …
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Functional, Biochemical and Molecular Analyses of the *Cold Stable Eye Lens Crystallins From the Antarctic Toothfish Dissostichus Mawsoni
… that toothfish alpha and beta crystallins are orthologues of mammalian counterparts, while gammas do not. Further separation, partial purification (ion-exchange chromatography) and analyses (isoelectric focussing and 2D SDS-PAGE) of the gamma crystallins has generated eleven sub-fractions, …
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An investigation of the role of CreB deubiquitinating enzyme in the regulation of carbon metabolism in Aspergillus nidulans
… in repressing and derepressing conditions. Orthologues of the co-purified proteins were identified in S. cerevisiae and humans. Functional annotation analysis revealed that proteins that preferentially interact with CreA in repressing conditions include histones and the histone transcription …
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Characterization of Drosophila Scap: Analysis of Mutants and Evidence for a Retention Factor
… In Drosophila dSREBP pathway there is no Insig orthologues. However, our data suggest that there should be an analogous component that retains dScap in the ER. In order to discover the putative retention factor and other modifiers of the dSREBP, I set up a high through-put genome-wide screen. …
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Spatial Expression Patterns of theSnmp Gene Family In Drosophila Melanogaster
<p>The CD36 orthologues, SNMP1 and SNMP2, are highly conserved throughout the Holometabola; including the insect lineages Coleoptera, Hymenoptera, Diptera, and Lepidoptera (Nicholls and Vogt, 2008). This evolutionary conservation provides intriguing, if indirect, evidence for their important …
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Identification and characterization of GRP50 : a novel Golgi-associated ankyrin repeat protein
… Homology searching tools reveal that no known orthologues of this protein have been identified in other species, indicating that it is not a member of a previously characterized family. GRP50 is abundantly expressed in fetal tissues and in adult brain, and is induced as part of the serum …
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Defining Molecular Mechanism of Egress in Cryptosporidium
… in our lab. Our aim was to analyze the C. parvum orthologues of molecules that mediate egress for other apicomplexans. Here we tested the hypothesis that calcium-dependent protein kinase 5 (CDPK5), subtilisin-like serine protease (SUB1), and cGMP-dependent protein kinase G (PKG) are essential for …
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MicroRNA cloning and bioinformatic analysis
… of 75 miRNA genes in the C. elegans genome, and orthologues for a majority of these were computationally identified in the C. briggsae, D. melanogaster or H. sapiens genomes. Northern analysis was used to confirm and analyze the expression of these miRNAs. The data set has implications for …
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Evolutionary and functional analysis of transcription factors controlling leaf development
… groups of homologues and 34 Possible Groups of Orthologues (PoGOs). bHLH64 is a gene highly expressed in early sink leaves, its expression is downregulated during the mature-to-senescent transition. Null mutants of bHLH64 are characterized by delayed bolting when compared to the wild-type; this …
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Molecular characterisation of XvRG6 and XvRF17 genes isolated from the resurrection plant Xeropyta viscosa
… responsive to stress. Since there are no known orthologues of these genes, their respective roles in X. viscosa remains speculative.
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Phylogenetic and developmental studies into the evolution of an insect novelty
… in the segment of the fly. Having identified orthologues of the genes in Tribolium using the genome sequence on BeetleBase, their expression patterns are examined in the beetle. Genes with conserved expression are deemed good candidates for a more widespread role in patterning the segment.
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Biochemical and Structural Characterization of the Atg8/LC3 Lipidation Pathway
Atg8 and its eukaryotic orthologues LC3 and GARBARAP family proteins (referred here to Atg8 family proteins) play crucial roles in autophagy through their covalent ligation to lipids, typically phosphatidylethanolamine (PE), in a process known as lipidation. Lipidation of Atg8 family proteins …
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