Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 17 of 17 for “"Origin recognition complex (ORC)"”.
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Keep the ORCs at bay : how eukaryotic cells ensure one round of DNA replication per cell cycle
… helicase-loading proteins: Mcm2-7, Cdc6, and the Origin Recognition Complex (ORC). Phosphorylation of free Mcm2-7 and Cdc6 leads to their removal from the nucleus (Mcm2-7 by nuclear export and Cdc6 by protein degradation). However, phosphorylated ORC remains in the nucleus bound to origins.
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Modeling Multi-factor Binding of the Genome
… factors (TFs), and other proteins and protein complexes, such as the origin recognition complex (ORC). DBFs compete with one another for binding along the genome, yet many current models of genome binding do not consider different types of DBFs together simultaneously. Additionally, binding is …
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Phosphorylation of the replicative helicase by the S-phase kinase, Dbf4-Cdc7, at origins of replication in Saccharomyces cerevisiae
… steps, selection of sites of initiation (called origins) and activation of these selected sites. Selection of origins occurs in late M- and early Gl-phase and is mediated by the origin DNA-binding protein called the Origin Recognition Complex (ORC). ORC recruits several proteins to the DNA, …
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Changing protein-DNA interactions promote ORC binding site exchange during replication origin licensing
… DNA replication proceeds bidirectionally from origins of replication. At the heart of this process is the eukaryotic replicative helicase, Mcm2-7, which leads the replication process by unwinding the DNA strands at the front of the replication machinery. To prepare for bidirectional …
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Delineation of the molecular mechanisms underlying DNA replication initiation and changes in gene copy number during Drosophila development
… the mechanisms by which binding of the origin recognition complex (ORC) and replication fork inhibition give rise to locally underreplicated regions in Drosophila polyploid tissues. We identified copy number changes genome-wide in two additional polyploid tissues and compared our results …
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An ORC Flip Enables Bidirectional Helicase Loading
… replication is the licensing of all potential origins by loading two inactive Mcm2-7 replicative DNA helicases around the origin DNA in a head-to-head double hexamer. This conformation prepares the helicases to initiate bidirectional replication upon entry into S-phase. Interactions between …
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Impact of DNA accessibility and sequence on eukaryotic origin licensing
Eukaryotic DNA replication is a complex process that requires the coordination of many different proteins to ensure duplication of the genome occurs rapidly and only once during each cell cycle. Establishing a pair of replication forks moving apart from a single starting location is essential for …
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The role of Orc6 in ORC binding-site switching during helicase loading
… The first step in eukaryotic DNA replication is origin licensing, during which the origin recognition complex (ORC) coordinates loading of two Mcm2-7 hexameric helicases in opposite orientations to form the Mcm2-7 double hexamer. These Mcm2-7 double hexamers mark sites of eventual bidirectional …
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Analysis of metazoan DNA replication initiation using Drosophila gene amplification as a model system
… follicle cells is an excellent model to study origin specification and developmental regulation of DNA replication in vivo. We mapped all follicle cell amplicons using a comparative genomic hybridization strategy and identified two new amplicons. We determined the precise localization of the …
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Protein-Protein Interactions in Cell Cycle Proteins: An <i>In silico</i> Investigation of Two Important Players
… Cdc6, an essential replication factor that binds origin recognition complex (ORC) and together these proteins work as a helicase loader. Regulation of Cdc6 is required for proper cell division and is carried out by interacting with proteins such as Cdc4 and Clb2. The lack of structural information …
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Genome-wide Footprinting Uncovers Epigenetic Regulatory Paradigms by Revealing the Chromatin Occupancy Landscape
… can capture nucleosomes partially unwrapped or complexed with chromatin remodelers. Stereotypical DNA-binding footprints are evident across all promoters, even at low-transcribed and silent genes. By aggregating the chromatin profiles across transcription-factor--binding sites, we precisely …
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Role of WD domain-containing proteins in cell cycle progression
… how two WD domain-containing proteins, LRWD1/ORCA and RFWD3, regulate different aspects of cell cycle progression. In eukaryotic cells, the duplication of the genome starts from the loading of a six-subunit complex, Origin Recognition Complex (ORC), onto replication origins on the chromosome. …
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Protein-DNA Binding: Discovering Motifs and Distinguishing Direct from Indirect Interactions
… through interactions between proteins or protein complexes and DNA. Although a lot is known about the interacting proteins and their role in regulating transcription and replication, the specific DNA binding motifs of many regulatory proteins and complexes are still to be determined. For this …
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Role of ORCA and ORC in chromatin organization and DNA replication
In eukaryotes, Origin recognition complex (ORC) proteins establish the pre-replicative complex (pre-RC) at the origins and this is essential for the initiation of DNA replication. In human cells, ORC is a highly dynamic complex with many separate functions attributed to sub-complexes or individual …
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Interaktionen und Lokalisationen der Replikationsproteine der Maus
… werden kann. Im ersten Schritt bindet der „origin recognition complex“ (ORC) an Replikationsorigins innerhalb chromosomaler DNA, wodurch eine Assemblierung des präreplikativen Komplexes an diesen Startpunkten ausgelöst wird. An den ORC lagern sich anschließend die Proteine CDC6 und …
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Analyse des Replikationsprozesses der Maus
… hochkonservierter Prozess. Zuerst bindet der „origin recognition complex“ (ORC) an Replikationsstartpunkte chromosomaler DNA und stellt das Startsignal für die Assemblierung des präreplikativen Komplexes (pre-RC) dar. Anschließend assoziieren die Initiationsfaktoren CDC6 und CDT1 mit dem ORC. …
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DNA Replication of the Male X Chromosome Is Influenced by the Dosage Compensation Complex in Drosophila melanogaster
… the genome. These start sites are called origins of replication, and they are initiated in a temporal manner throughout S phase. How these origins are selected and regulated is poorly understood. Saccharomyces cerevisiae and Schizosaccharomyces pombe have autonomously replicating sequences …