Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 216 for “"Organoids"”.
-
Engineering Organoids for Stem Cell Maturation
… sinusoidal endothelial cells, are present in the organoids, they secrete signaling molecules that synergistically mature iHLCs. Relying solely on intercellular secretion from the cells provides a systematic and reproducible approach to generate mature iHLCs for drug, disease, and patient-specific …
-
Elucidating Astrocytic Diversity in Human Cerebral Organoids
… newfound astrocytic diversity in human cortical organoids grown at the air-liquid interface (ALI-COs) for up to 495 days in vitro (DIV). It shows that ALI-CO development displays considerable similarities with the human fetal cortex and undergoes significant gliogenesis at 200DIV. In particular, …
-
Consequences of Plk4 overexpression in mouse pancreatic organoids
… cell biology using mouse pancreatic ductal organoids. In accord with previous observations, immunofluorescence experiments confirmed that Plk4 overexpression leads to centrosome amplification and reduction in primary cilia number. I also investigated the consequences of long-term Plk4 …
-
ORGANOIDS AS TOOLS TO STUDY BRAIN DEVELOPMENT AND DISEASE
Human brain organoids (ORG) are a widely used in vitro model that contribute to our knowledge about the biology and pathophysiology of the nervous central system (CNS). They are 3D multicellular clusters that mimic the cytoarchitecture and the developmental pathways that occur in vivo. The most …
-
Traumatic Injury-Induced Cell Responses in Human Cortical Organoids
… progenitors and astrocytes in 150 DIV cortical organoids. Our results highlight potential differences in fetal astroglia-microglia interactions to that found for the adult brain and provide a unique platform for cell interaction-based studies with broad relevance to TBI pathogenesis and …
-
OrganixInsights: High throughput imaging and high content screening of organoids
… disease progression, and therapeutic response. Organoids replicate key structural and functional characteristics of in-vivo tissues, including cellular heterogeneity, spatial organization, and extracellular matrix interactions. However, high-resolution imaging of organoids generates large …
-
Investigating Vascular Patterning and Regression in Kidney Development and Organoids
Pages 74-127 are misnumbered as pages 75-128.
-
Cerebral Organoids as 3-Dimensional Grafts in Traumatic Brain Injury
… recent emergence has led researchers to examine organoids’ potential as tissue grafts to regenerate the lost tissues in the brain following traumatic brain injury. In this dissertation, we sought to understand the interaction between the organoid and host following transplantation as a tissue …
-
Modeling colorectal cancer initiation using human iPSC-derived intestinal organoids
… stem cell (hiPSC)-derived human intestinal organoids (HIOs) are increasingly utilized as in-vitro models for studying colorectal cancer (CRC). Prevalent oncogenic drivers in CRC include mutations in EGFR (L858R), KRAS (G12D) and CTNNB1 (S33C) which are known to contribute to neoplastic …
-
Investigating the control of epithelial polarisation in human kidney organoids
… to human kidney development, using human kidney organoids as a model. Grown from induced pluripotent stem cells, intermediate mesoderm-like cells are aggregated into spheres of 1000 cells, and over the course of four days spontaneously polarise and organise into tube structures in the absence of …
-
Human Liver Organoids as models for investigating drug-induced liver injury
… the advent of 3D epithelial structures called organoids, yet its use in the liver safety field is currently unexplored. Liver organoids have the great advantage to be highly proliferative, to maintain the identity of the tissue of origin, and to retain the bipotential ability to differentiate …
-
SPINAL MUSCULAR ATROPHY ORGANOIDS REVEAL DEVELOPMENTAL DEFECTS RESCUED BY ANTISENSE OLIGONUCLEOTIDES TREATMENT
… we generated SMA type 1 cerebral and spinal cord organoids (SCOs) to recapitulate human-specific early neurodevelopmental features. Single-cell transcriptomics revealed a pervasive transcriptional alteration in multiple cell populations, along with drastic changes in motor neuron gene expression. …
-
IDENTIFICATION OF CANCER- AND METASTASIS-RELATED EPIGENETIC DYNAMICS USING PATIENT-DERIVED ORGANOIDS
… in colorectal cancer (CRC) patient-derived organoids (PDOs) with respect to the normal counterpart that are also conserved among different CRC subtypes, suggesting the presence of shared epigenetic mechanisms that are activated despite heterogeneous mutational landscapes. Aiming at …
-
MODELLING GBA1-RELATED PARKINSON¿S DISEASE PATHOLOGY IN PATIENT-DERIVED MIDBRAIN ORGANOIDS
… of Lewy bodies and Lewy neurites in GBA1-mutated organoids. We show that α-synuclein extracted from Parkinson’s samples displays seeding activity in vitro and, following inoculation in unaffected organoids, propagates the pathology. Finally, we demonstrate pharmacological rescue of pathological …
-
Modelling of the Human Corticospinal Motor Axis and Its Injury Using Organoids
… utilising stem cell derived cerebral and spinal organoids to recreate the corticospinal neuraxis and traumatic injury responses. To develop this corticospinal neuraxis, an optimised spinal organoid differentiation protocol was established. Adapting from previous protocols, its shown that by …
-
Characterising molecular and cellular mechanisms of human brain evolution using brain organoids
… largely unknown. In this work we show that brain organoids can be used to identify and functionally test mechanisms underlying human-specific features of brain development. Through a meta-analysis of comparative genomics and transcriptomics, we identified candidate regulators of human brain …
-
ADVANCING IN SITU CRYO-ET TO THE NEXT DIMENSION: TOMOGRAPHY ON 3D ORGANOIDS
… alternative is the use of three-dimensional organoids, which present a balance between physiological relevance and technical capabilities. Organoids recapitulate key aspects of tissue architecture and physiology, thus bridging the gap between cellular and tissue contexts. Additionally, they …
-
Engineering human hepatic organoids for in vitro liver modeling and implantable cell therapies
… organoid culture conditions by generating organoids from eight different adult donors. Mature organoids accurately model the phenotype and morphology of hepatocytes in the adult liver, express a mature liver transcriptome distinct from fetal hepatocytes, and exhibit adult functions such as …
-
INVESTIGATING THE ROLE OF FEZ1 IN EARLY HUMAN BRAIN DEVELOPMENT USING CEREBRAL ORGANOIDS
… during early brain development by human cerebral organoids model and loss of function study. Transcriptome analysis revealed genes associated with neuronal development and synaptic function were differentially expressed in FEZ1 knockout organoids, suggesting FEZ1 participated in early forebrain …
Page 1 of 11