Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 14 of 14 for “"Orai1"”.
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Stoichiometry of the interactions between endogenous Orai1 and STIM1 during store-operated Ca2+ entry
… (MCS), STIM1 forms puncta that trap and activate Orai1 Ca2+ channels in the PM, allowing Ca2+ to flow into the cytoplasm and slowly replenish Ca2+ in the ER. Elucidating the binding stoichiometry of STIM1 and Orai1 is essential for understanding Orai1 gating and mechanisms of SOCE. Most previous …
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Remodelado de la entrada de calcio en la angiogénesis: papel de las proteínas ORAI1 Y SARAF
… SARAF, el emergente factor regulador de SOCE, y Orai1, la subunidad formadora del poro de los canales de SOCE. En este estudio demostramos que la inhibición de SOCE usando GSK-7975A (GSK), bloquea el crecimiento de los brotes a partir de anillos de aorta de rata cultivados ex vivo, la migración y …
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S-Acylation Is A Key Regulator of Orai1/Stim1-Mediated Store-Operated Calcium Entry In T Cells
<p>Orai1 and STIM1 proteins are the essential components of the Ca<sup>2+</sup> release activated Ca<sup>2+</sup> (CRAC) channel which is required for store-operated Ca<sup>2+</sup> entry (SOCE) in T cells and subsequent signaling events leading to T cell activation, proliferation, and …
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STIM Protein Coupling Domains and The Activation of Orai Channels
… and STIM2. We narrowed these differences in Orai1 gating to a strategically exposed phenylalanine residue (Phe-394) in SOAR1, which in SOAR2 is substituted by a leucine residue. Remarkably, in full-length STIM1, replacement of Phe-394 with the dimensionally similar but polar histidine …
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Store-Operated Calcium Entry And Its Function During Fertilization In Porcine Eggs
… entry during fertilization, we studied Orai1, the proposed channel component of this signaling pathway. Through RT-PCR we demonstrated the presence of Orai1 transcripts in pig eggs; the results of Western blot analysis further confirmed that Orai1 is expressed in pig eggs. In addition, …
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Remodelado de la entrada de calcio operada por los reservorios y su regulación por micrornas en la reestenosis vascular
… revelaron un incremento de la expresión de Orai1, STIM1 y Saraf en las arterias lesionadas por angioplastia. Además, demostramos una colocalización/interacción entre Orai1 y Saraf en la zona de la neoíntima que presenta una mayor proliferación celular. La inhibición de expresión de Orai1 y …
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The Secretory Pathway Calcium ATPase 2C promotes increased cytosolic calcium levels
… for SPCA2 in increasing Ca2+ influx involving Orai1 in breast cancer. The goal of this study was to determine the effects of SPCA2C on Ca2+ homeostasis. Here, epitope-tagged SPCA2C was expressed in HEK293A cells. Co-immunofluorescence (Co-IF) determined subcellular localization. Using Fura2 …
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Structure-based development of novel chemical scaffolds as inhibitors of the store-operated calcium entry pathway
… SOCE is mediated by Orai proteins, mainly by Orai1, the pore-forming protein of the CRAC (Calcium release-activated calcium) channel, which is activated following store-depletion by aggregated stromal interaction molecule (STIM) proteins that are localised within the ER-membrane and serve as …
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Rapid Non-genomic Actions of Cortisol in Fish
… site, and this corresponded with an increased ORAI1 (protein subunit of CRAC) expression at the site. Also, an increase in cell proliferation of ORAI1 expressing cells and blastema formation was evident at 24 h in response to cortisol stimulation, but absent in the GR knockout and MR knockout …
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Identification and Characterisation of New Mouse Models for Hearing Loss
… one ENU-induced coding lesion, which is in the Orai1 gene that codes for a component of the calcium activated calcium entry (CRAC) channel. These two novel mouse models highlight the important role for ENU mutagenesis in disease gene discovery.
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Crosstalk Signaling Between cAMP and Calcium on the Leading Edge of Migrating Human Arterial Vascular Smooth Muscle Cells
… actions of the SOCE promoting machinery (i.e. Orai1/STIM1), a Ca2+-activated adenylyl cyclase (ADCY8), PKA and the A-kinase anchoring protein (AKAP79). Based on our findings, we suggest that inhibiting the actions of PDE1C may represent a valid novel therapeutic approach to decrease the burden …
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Pharmacological regulation and function of store-operated calcium channels
… clustering, but also nonselectively inhibited ORAI1/2/3 and TRPC3/4/5/6 channels.The sensitivity of TRP channels to metal ions was also investigated using patch clamp. Micromolar Cu²⁺ significantly increased the currents of TRPC3/4/5/6 channels. The glutamic acid (E542/E543) and cysteine (C554) …
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The Role of MRGPRX2/Mrgprb2 in Regulating Mast Cell Function
… we" present" an" evidence" that" STIM1/Orai1" plays" an" important" role" in" MRGPRX2" mediated" calcium" influx" and" mediators"release"when"stimulated"with"LLE37"and"SP."" Moreover,"this"dissertation"showed"naturally"occurring"missense"mutations" of" MRGPRX2" both" within" and" …
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HUMAN CLCA2 MODULATES THE CONDUCTANCE OF CALCIUM-ACTIVATED CHLORIDE CHANNELS BY REGULATION OF INTRACELLULAR CALCIUM
… complex with mediators of SOCE, STIM1 and ORAI1. These results explain the CaCC-enhancing effects of CLCA family members and suggest a broader role in other calcium-dependent processes. Understanding the modulatory relationship between these molecules may lead to better therapies for airway …