Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 100 for “"Optical tweezers"”.
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Assembly of Structures using Optical Tweezers
… dissertation, we explore the potential of an optical positioning and linking (OPAL) platform based on optical tweezers and a biochemical linking mechanism. Optical tweezers (OT) are a powerful technique that permit the non-contact manipulation of a range of objects, spanning from the nanoscale …
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High Resolution Optical Tweezers for Biological Studies
… biophysical tool with such capabilities is an optical tweezers. Optical tweezers is a non-invasive all-optical technique in which typically a dielectric microsphere is held by a tightly focused laser beam. This microsphere acts like a microscopic, three-dimensional spring and is used as a …
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Optical tweezers for signal detection and micromanipulation
… explores new multi-disciplinary applications of optical tweezers in the physical and biological sciences. Firstly, the three dimensional trapping of partially silvered sphere in a standard TEM00 optical trap was characterised. These spheres were then coated with an azo dye such that …
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High-speed video microscopy in optical tweezers
Optical tweezers have become an invaluable tool for measuring and exerting forces in the pico-Newton regime. Force measurements have in the past concentrated on using only one trapped particle as a probe, partly due to the difficulties in tracking more than one par- ticle at high enough frame rate. …
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Tools to study the kinesin mechanome using optical tweezers
Molecular motors play an important role in driving some of the most complex and important tasks in biological systems, ranging from transcribing RNA from a DNA template (Polymerases) to muscle contraction (Myosin) and propelling bacteria (Flagellum). Key to the understanding of the fundamental …
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An automated and user-friendly optical tweezers for biomolecular investigations
Optical tweezers are not new to biological applications but building a tweezers which is versatile, automated, precise, stable and user-friendly is quite challenging. Such a tweezers usually require not only a hefty budget but vast understanding of engineer- ing of optomechanical systems. I …
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Membrane tether formation from outer hair cells with optical tweezers
… elucidate the mechanism of OHC electromotility. Optical tweezers were used to characterize the mechanical properties of OHC plasma membrane (PM) by pulling tethers. A greater force was required for tether formations from OHC lateral wall (499 pN +/- 152) than from OHC basal end (142 pN +/- 49), …
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Modeling and Experimental Techniques to Demonstrate Nanomanipulation With Optical Tweezers
… of effective prototyping tools at the nanoscale. Optical trapping is well established for flexible three-dimensional manipulation of components at the microscale. However, it has so far not been demonstrated to confine nanoparticles, for long enough time to be useful in nanoassembly applications. …
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Antibody-Antigen assay design for combined optical tweezers and fluorescence
The recent development in combined optical trapping and fluorescence technology promises to enable unbindinig force studies of receptor-ligand interactions, whose specificity play a crucial role in the function of many biological systems. This thesis focuses on the development of assay designs for …
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Exploring the mechanome with optical tweezers and single molecule fluorescence
The combination of optical tweezers and single molecule fluorescence into an instrument capable of making combined, coincident measurements adds an observable dimension that allows for the examination of the localized effects of applied forces on biological systems. This technological advance had …
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Optical tweezers using the Texas Instruments' Digital Micromirror Device(tm)
Thesis (M.Eng. and S.B.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, 2001.
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Ion flow and membrane tension studies with optical tweezers and nanopipettes
… setup and the studies this has enabled. Optical tweezers, nanopipettes, and fluorescence were all combined to study the effect of salt species on nanoscale voltage driven flows, the mechanical behaviour of cell membranes, and the electrical activity from various cells. Voltage driven, or …
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Probing DNA-Induced Colloidal Interactions and Dynamics with Scanning-Line Optical Tweezers
… The pair-interaction energies measured with our optical tweezers instrument can be modeled quantitatively with a conceptually straightforward and numerically tractable model, boding well for their application to direct self-assembly. In addition to understanding the equilibrium interactions …
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Single-molecule optical tweezers studies of the mechanism of DNA unwinding by a model helicase
Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2023-09-01 without embargo terms
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Colliding and moving Bose-Einstein condensates : studies of superfluidity and optical tweezers for condensate transport
… condensate. The impurities were created using an optical Raman transition, which transferred a small fraction of the atoms into an untrapped hyperfine state. A dramatic reduction in the collisions between the moving impurities and the condensate was observed when the velocity of the impurities was …
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Using optical tweezers, single molecule fluorescence and the ZIF268 protein-DNA system to probe mechanotransduction mechanisms
Optical tweezers instruments use laser radiation pressure to trap microscopic dielectric beads. With the appropriate chemistry, such a bead can be attached to a single molecule as a handle, permitting the application of force on the single molecule. Measuring the force applied in real-time is …
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Development of a Novel Gradient-Force Tapered Fibre Optical Tweezers System for 3D Optical Trapping at Near Horizontal Fibre Insertion Angles
The use of optical fibre as a mechanism for the delivery of the trapping laser beam to the sample chamber significantly reduces both the size and the build costs of “Optical Tweezers”. Furthermore, the use of fibre facilitates the decoupling of the optical trapping beam from the microscope optics, …
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Photonic Tweezers for Optical Manipulation of Cells and Tissues
Optical tweezers represent a powerful tool for studying forces in biological samples. In this thesis, I explored applications of optical tweezers that pushed the technique beyond its limitations. In fact I worked on extending the range of forces applicable by the tweezers and on applying optical …
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Spatial Emission and Polarization Control in Integrated Photonics for Optical-Trapping and Trapped-Ion Systems
… results in fields including biophotonic optical tweezers and trapped-ion quantum systems. However, the majority of these demonstrations, while offering advantages in size, cost, and dense integration, lag behind their bulk-optic counterparts, limited by a lack of critical advanced …
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