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Showing 1 to 20 of 112 for “"Operons"”.
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Identification of Operons in clostridium difficile
… data to accurately predict and analyze operons in C. difficile. Recently, COSMO, an operon predictor developed using ML techniques, was employed to investigate the operon structures and their roles in drug resistance and pathogenicity in Mycobacterium tuberculosis. In this study, the …
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Mutagenese des nuo-Operons von Escherichia coli
… NuoN, die von den Genen des 15 kbp großen nuo-Operons kodiert werden. Die Gene nuoC und nuoD sind in E. coli zu einem Gen fusioniert. <br> <br>Das nuo-Operon wurde in den Expressionvektor pBAD33 kloniert und die nuo-Gene extrachromosomal exprimiert. Dies führte zu der Produktion eines …
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Identification and characterization of the ribosomal RNA operons from Rhodobacter sphaeroides
The three ribosomal RNA operons present in the facultative photoheterotroph Rhodobacter sphaeroides were identified, cloned and sequenced. DNA sequence analysis has identified the 16S, 23S and 5S rRNAs, two tRNAs (ile and ala) in the 16S-23S spacer region, and an f-met tRNA immediately following …
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The Roles and Regulation of the Redundant Phenazine Biosynthetic Operons in Pseudomonas aeruginosa PA14
… enzymes are found in two redundant 7-gene operons. These operons, phzA1-G1 (phz1) and phzA2-G2 (phz2), encode two sets of proteins that catalyze the synthesis of phenazine-1-carboxylic acid (PCA), the precursor for all other phenazine derivatives. Although the phz1 and phz2 operons are …
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Umwelt-Genomik als Quelle für die Isolierung von neuen Operons und Genclustern aus mikrobiellen Konsortien
… die Isolierung von neuartigen Biotinbiosyntheseoperons aus mikrobiellen Konsortien mit Hilfe kombinierter Anreicherung und direktem Klonieren. Hierzu wurden Cosmid-Genbanken aus Anreicherungskulturen mit einer Fragment-Größe von ca. 30 kb angelegt. Anschließend wurden die Genbanken auf Klone …
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Adding to the recombineering toolbox: interrogating internal transcriptional complexity in Caenorhabditis elegans operons via recombineered fosmid-based reporters
Operons are common in prokaryotic genomes but, except for Nematoda, are uncommon in eukaryotes. Nematoda operons comprise gene clusters, containing between two to eight genes, with short, approximately. 100 bp, intergenic regions. Operons are common in C. elegans with an estimated 17% of genes …
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Characterization of the <i>glpD</i> and <i>glpEGR</i> operons of <i>Escherichia coli</i> k-12
The proteins required for catabolism of glycerol 3- phosphate are encoded by the genes of the <i>glp</i> regulon of Escherichia coli and are under negative transcriptional regulation by the <i>glpR</i>-encoded repressor. The <i>glpR</i> gene is adjacent to, and is transcribed divergently from, …
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Action at a distance for specific repression of the glpD and glpTQ genes, and organization of the glpEGR genes of Escherichia coli K-12
… The divergently transcribed glpACB and glpTQ operons encode the subunits of the anaerobic glycerol-P dehydrogenase, glycerol-P transporter, and glycerophosphodiesterase, respectively. The glpD operon is controlled by cooperative binding of glp repressor (GlpR) to tandem operators that cover …
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INVESTIGATION OF THE NOVEL ANTITERMINATION MECHANISM OF LOAP, A NUSG SPECIALIZED PARALOG
… enhance the expression of ribosomal RNA operons, bacteriophage operons, and CRISPR arrays. Specialized NusG paralogs, such as LoaP, RfaH, or UpxY, are widespread in bacteria and likely form their own unique antitermination complexes. RfaH, the only well-studied paralog, binds to a paused …
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Genetic and Biochemical Analysis of Methyltransferase Gene Regulation in Methanosarcina Acetivorans C2A
… spp. have three mtaCB (mtaCB1, 2 and 3) operons. In order to understand why these organisms maintain genes encoding isozymes we used the three mtaCB operons in M. acetivorans C2A as a model. Mutant analysis established that these operons encode bona fide methanol MT1s, which have distinct …
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Analysis of Chromosomal Rearrangements in Host Specific Salmonella Serovars
… tolerate inversions, or rate of inversion at rrn operons in either generalist or host-specific serovar of Salmonella enterica. Analysis of inversions at rrn operons suggests that both generalist and host-specific serovars undergo inversions at a rare frequency in standard laboratory conditions. It …
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The regulation of denitrification in P. denitrificans
… NnrR positively regulated the nir and nor operons and inhibited the expression of the nar and nos operons, in the latter case due to substrate unavailability. Finally, NarR positively enhanced the expression of the nar operon during the initial stage of anaerobicity. Additionally the …
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OperomeDB: database of condition specific transcription in prokaryotic genomes and genomic insights of convergent transcription in bacterial genomes
… we developed a database for the prediction of operons for bacterial genomes using RNA-seq datasets, we predicted operons for bacterial genomes. RNA-seq datasets with different condition for each bacterial genome were taken into account and predicted operons using Rockhopper. We took RNA-seq …
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Structural Analysis of the TOL pDK1 xylGFJQK Region and Partial Characterization of the xylF and xylG Gene Products
… between corresponding regions of different operons, combined with variable base and codon usage patterns within and between operons, provides additional support for the idea that the upper and lower operons encoding enzymes of aromatic pathways have evolved independently of one another and …
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Novel Ribosome Biogenesis in the Lyme Disease Spirochete Borrelia burgdorferi
… rRNA transcripts from the unusual rRNA gene operons of Borrelia burgdorferi. In most bacteria, the three rRNA transcripts (16S, 23S, and 5S rRNAs) that form the ribosome are produced as a single transcript from an operon with minimal spacing between genes. In the B. burgdorferi genome, …
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Novel Ribosome Biogenesis in the Lyme Disease Spirochete Borrelia burgdorferi
… rRNA transcripts from the unusual rRNA gene operons of Borrelia burgdorferi. In most bacteria, the three rRNA transcripts (16S, 23S, and 5S rRNAs) that form the ribosome are produced as a single transcript from an operon with minimal spacing between genes. In the B. burgdorferi genome, …
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Intramembrane signal transduction and cell envelope stress response in <i>Bacillus subtilis</i>
… werden die letzte! n vier Gene des Operons von einem schwachen konstitutiven Promotors vor liaG exprimiert, wodurch das Vorhandensein des Zweikomponentensystems in der Zelle sichergestellt wird. Der aktivierte Response Regulator LiaR induziert die Expression des lia Operons, indem er …
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